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Temperature

60°C

45°C

Absorbance

0.02 AU max. at 330nm

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Cell isolation

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Application_4

Chromatography system|Manual

Cell isolation

Centrifugation

Chromatography system

Applications

LPLC: suitable

gas chromatography (GC): suitable

HPLC: suitable

solid phase extraction (SPE): suitable

Application_5

Chromatography system|Manual

Base Style

Flat Bottom

Bed Support

Filter

Net

Bed Height

25 mm

100 mm

600 mm

300 mm

Bed Volume

1 mL

320

120

5 mL

Average sample loading capacity

50 mg

400 mg

Assay Percent Range

99.8% min by GC

Bed Dimensions

16 x 25 mm

26 x 600 mm

7 x 25 mm

16 x 100 mm

16 x 600 mm

1.6 x 2.5 cm

Capacity

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

=25 % absorption capacity (water)

1.7 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.35 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

200 µL

1.8 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

Autoclavable

Not Autoclavable

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

Up to 20 mg histidine-tagged protein

~ 13 mg ovalbumin

Boiling Point

2230 C

189°C

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

Bore Size (mm)

Brand

USA Scientific Brand

Axygen®

CarbonLoad

15%

11%

18%

Autoignition Temp

800 °F

Avoid Using

Chelating agents such as EDTA, EGTA , citrate

Bed Height With One Adaptor

579 mm-609 mm

Bed Height With Two Adaptors

0 to 200 mm

0-280 mm

Buffer

Running Buffers

CAS_NO

112945-52-5

9003-70-7

112926-00-8

1343-88-0

9052-95-3

104219-63-8

69011-20-7

7631-86-9

39389-20-3

CAS Registry Number 1

67-68-5

7631-86-9

Chem AlertStorageSymbol

Gray

Chemical Name or Material

Silica Gel

Dimethylsulfoxide

Chemical Stability

Commonly used aqueous buffers

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Chromatography technique

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Affinity coupling

Group specific affinity

Histidine-tagged protein purification

Assay

=99%

Closure Color

Black

Silver

Closure Type

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Crimp Cap

Screw cap

Screw

ClosureMaterial

Polypropylene

Column Type

capillary chiral

capillary polar

capillary non-polar

packed GC

Mixed Mode

capillary extremely polar

Reversed Phase

ChEBI

CHEBI:28262

Composition

Discovery ® DSC-18, 400 mg

bed wt., 500 mg

magnesium sulfate, 150 mg

Tris Base, Sodium Chloride

Diameter Metric

2.1 mm

4.6 mm

BioProcess resin

yes

EC Number

215-681-1

238-878-4

215-609-9

231-545-4

262-373-8

200-838-9

231-153-3

Endcapped

Proprietary

Yes

Feature

closure type screw top vial

septum type Thermogreen ® LB-2

Not Endcapped

preconditioned

closure type black phenolic solid top cap

without needle Luer tip

Endcapped

syringe type Sample Lock

needle type N (cemented)

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

1.6 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 20 ml/min|< 20 ml/min

1 mL/min - 4 mL/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

2 ml/min - 10 ml/min

Format_6

Prepacked column

Prepacked columns

Bulk resin

Kits

Chemical Substance ID

24852837

329759181

329763461

24870506

24857358

329763462

329750331

329759187

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Chemical/Physical Resistance

Temp. Limits:. -10 C to 180 C isothermal and programmed

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Closure Material

Plastic

Aluminum

Polyethylene

Polypropylene

Color

Colorless to slightly yellow

Grey

Yellow

Gold/Brown

Colorless

Natural

Red

Gold

Silver

Brass

Amber

Black

Clear

White

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

ClosureSize

11 mm

9 mm

Color

N/A

Column volume

8 mL

1 mL

Closure Size

11 mm

13 mm

20 mm

9 mm

ClosureType

Screw Cap

Screw

Snap Cap

Screw Top

Column Format

Analytical Column

Analytical Column, EASY-Spray

Column i.d.

10 mm

16 mm

50 mm

26 mm

5 mm

Compatibility

Compatible with most QuEChERS shakers.

Concentration

20 X

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Density Max.

1.2 g/ml

Decomposition Information

>190°C

Diameter Metric Opening

11 mm

Diameter Metric Outer

12 mm

8 mm

15 mm

Diameter MetricOpening

9 mm

0.12 µm

0.20 µm

Density

1.100g/cmÂ³

Diameter Metric Inner

6.6 mm

12 mm

15 mm

Diameter Metric

75 µm

Column Capacity

< 1 mg/mL

COLOR

Blue

Endotoxin Activity Max.

0.12 EU/mg

Flow Velocity

< 450 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

â‰¤ 600 cm/h

< 300 cm/h

< 150 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

<1800 cm/h

15 cm/h|15 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

Empirical Formula

MgO3Si

Flash Point

87°C (188.6°F)

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Form

powder or crystals

liquid

powder

fine crystals

fine powder

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

MgO₃Si

Service & Support

O2Si

Exclusion Limit Peptides

4 x 10Â³

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Formula Weight

78.13

100.39

60.08

Format

matrix material

For Use With

ELSD, UV Detector

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Estimated Shelf Life from Manufacture Date

5 years

FilmThickness

1 µm

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

Format

Liquid

H-Codes

H371

H351

H315-H319-H335

H372

H317

H319

H373

Health Hazard 2

WARNING!

Grade

high-purity grade

Standard

high-purity grade (7754)

high-purity grade (Davisil Grade 923)

HPLC

certified reference material

Grade

Technical Grade

High-purity Grade (Davisil Grade 643)

Height Metric

32 mm

45 mm

Hold-up Volume of Membrane

<20 Î¼l

<10 Î¼l

<5 Î¼l

Height English

1.77 in.

1.25 in.

Identification

Pass Test

Graduations

0.5 mL

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Height Metric

40 mm

Housing

Polypropylene (PP)

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Inlet Connector

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

2 mm (0.08 in)

Insert Type

350µL Fused Insert

Fused Insert

250µL Fused Insert

Pre-Slit

Includes

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Instructions supplied with the concentractors

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Connectors for high- and low-pressure chromatography equipment and instructions.

IOD-Codes

Includes

100 vials and 100 seals with septa

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

Vials and Closures

Ionic Capacity

0.18-0.25 mmol H⁺/ml medium

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.29-0.35 mmol Cl^-/ml medium

0.13-0.17 mmol/ml

0.11â€“0.16 mmol (Cl^-)/mL resin

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Intrinsic Viscosity

~ 17

Ion Exchanger Type

Strong anion exchanger

Weak anion

Weak and strong anion

Weak anion exchanger

Multimodal weak cation exchanger

Strong cation exchanger

Weak cation exchanger

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Alkali-stabilized Protein A-derived (E. coli)

Carboxymethyl

Cibacron Blue

Human IgG

Quaternary amine

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Diethylaminoethyl

Native protein A

TALONÂ® Superflow Cobalt-IMAC

Porcine heparin

Recombinant protein A (E.coli)

Sulphopropyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Ligand Density

~ 5 mg/mL resin

Approx. 13 Î¼mol/ml

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

2 mg Protein G/ml medium

6 mg rProtein A/ml medium

Kit Contents

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Aldolase (50 mg): 158 000 M_r
Ferritin (15 mg): 440 000 M_r
Thyroglobulin (50 mg): 669 000 M_r
Blue Dextran 2000 (50 mg

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Length Metric

60 m

500 mm

400 mm

457 mm

100 mm

250 mm

50 mm

Length Metric

750 mm

150 mm

30 m

51 mm

20 mm

Linear Formula

(CH₃)₂SO

MgO3Si

(C10H12 · C10H10 · C8H8 · C3H9N)X

SiO2

(C10H12 · C10H10 · C8H8)x

MarkingSpot

Yes

Material

polypropylene body

bevel tip stainless steel needle (point style 2)

Cellulose

Fused silica

polypropylene material (hardware)

PTFE-faced rubber liner

PTFE/silicone septa

Silica Gel

Stainless Steel

stainless steel hardware

VG2, 60% Vespel/40% Graphite

red PTFE/silicone septum

stainless steel hardware (column)

Tedlar ® PVDF film

white PTFE/silicone

white rubber liner

high-density polyethylene vial

Metal

PTFE/silicone

Rubber

stainless steel column

stainless steel holder

Borosilicate Glass

Polypropylene (PP)

PTFE/red rubber septum

stainless steel TD tube

clear glass

Amber Glass (Type 51A)

Plastic/Stainless Steel

PTFE

silicone body

Glass

Glass Body

stainless steel TD tube (includes screens)

PE frit (20 µm porosity)

PE frit (20/40 µm)

PTFE Luer lock

Brass

PEEK

Phenolic

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

polypropylene tube

(Multi-Layer Foil: (1) 0.002" Polyethylene (2) 0.0003" Aluminum Foil (3) Polyethylene (4) Aluminum (metalized) (5) 60 Gauge Nylon)

black polypropylene cap

clear glass insert

glass TD tube

PE frit

Polycarbonate (PC)

SilTite

stainless steel frit

clear glass vial

Glutathione Sepharose 4 B

Mixed

PTFE body

PTFE/silicone septum

polypropylene hardware

N/A

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Cross-linked agarose, 4%

agarose, 6%, spherical

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

cross-linked, 6% agarose particles

Highly cross-linked agarose

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Cross-linked dextran

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Ligand Concentration

~25 Âµmol phenyl/mL resin

~45 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

Ligand_7

Protein L

Butyl, octyl, phenyl

Protein G

Protein A, alkali stablilized

2-mercaptopyridine

Protein A

Protein A, alkali stabilized

Length English

2 in.

Max Temperature

340/360°C

40°C

330/350°C for Standard Columns; 430°C for Metal Columns

MDL Number

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00132722

MFCD00011232

MFCD00145586

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00149897

MFCD00211881

MFCD03791384

MFCD00132707

MFCD00132771

MFCD00146442

MFCD00084428

MFCD00132726

MFCD00145831

MFCD00145841

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

Cyanogen bromide activation

Triazine

Ether linkages and hydrophilic spacer arm

Ether linkages

Epoxy

Maleimide activation

Liner Material

PTFE/Silicone

None

Polypropylene

Marking Spot

Max Temperature English

122°F

Material

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

Polypropylene

PTFE Frit

100% Dimethyl Polysiloxane

StableFlex Fiber

Material Coating

Polypropylene

Material Plunger

Stainless Steel

Matrix

4% Agarose

6% Cross-Linked Agarose

Highly cross-linked 4% agarose

Rigid, cross-linked agarose, spherical

Max Pressure

4000 psi (275 bar)

2000 psi

14500 psi (1000 bar)

900 psi

5800 psi (400 bar)

11600 psi (800 bar)

17400 psi (1200 bar)

4500 psi (310 bar)

Max Temperature Metric

50°C

Melting Point

18.4°C

Length English

18 in.

Ligand Type

Monomeric C18

Max Temperature

445°C

Molecular Formula

C2H6OS

O2Si

Molecular Weight

60.08

178.13 (anhydrous basis)

0.0

78.129

100.39

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Length English Needle

2 in.

Operating Flow Rate, Max

< 4 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Operating pH

0 - 9

3.8 - 6.8

0 - 14

2 - 7.5

2 - 8

1 - 9

2 - 9

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

6.5 (5% in slurry)

Order code

SH30031.01

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

For research use only.

HiLoad columns are prepacked XK columns.

Percoll PLUS is for research use only.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Needle Gauge

16 g

Material Septa

PTFE/Silicone/PTFE

PTFE/Silicone

Pack size

2 x 1 ml 10% slurry

50 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

100 g

5 mL

500 mL

1 L

100 mL

1000 mL

1 Ã— 4.7 mL

2 x 1 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

5 Ã— 1 mL

1 piece

20 mL

300 mL

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Nanopolymer Hybrid Silica

Partical Size Dry

18 Âµm-111 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

Particle size, d_50V

~90 Âµm

34 Âµm

~ 9 Âµm

~90 Î¼m

~34 Âµm

~34 Î¼m

~75 Âµm

~85 Âµm

~ 8.6 Âµm

Osmolality

< 30 mOsm/kg

288-310 mOsm/kg

pH stability, operational

2â€“10

pH 6 to 10

3â€“10

2â€“13

4â€“12

2â€“12

2â€“9

4â€“13

4â€“9

3 to 12

3â€“13

3â€“12

3â€“9

Physical State

liquid

liquidSuspension

solid

Pore Size

110 Å pore diameter

60 Å pore size

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

0.7-0.85 cm 3 /g pore volume (approx.)

160 Å

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

100 Å

~0.55 mL/g pore volume

120 Å

90 Å

0.75 cm 3 /g pore volume

180 Å

60 +?

60 Å mean pore size

70 Å pore size

1000 Å

100 Å

150 Å

90 Å mean pore size

Particle Size

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

24-44 Âµm

24 Âµm-44 Âµm

60 Âµm-165 Âµm

60 Î¼m-160 Î¼m

2 µm

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

pH Range

8.9â€“9.9

5.5â€“7.5

8.5â€“9.5

Phase

Reversed Phase and Ion Exchange

Reversed Phase

Needle Point Style

Blunt

Operating Flow Rate, Recommended

0.075 mL/min

0.80 mL/min

0.5 mL/min

1.3 mL/min

p H

4 to 13

7.5 pH

2 to 8

2.5 to 7

Pack Size

Pressure/Flow Specification

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Outlet id

0.75 mm (0.029 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Packaging

Amber Glass

Packaging Layout

1, 6x1, 2.5 kg in poly bottle

1 kg in poly bottle

1 ft3 in fiber drum

25, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

100, 500 g in poly bottle

Percent Purity

≥99.9%

7.0 to 7.4

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

P-CODES

P280

P314

P305 + P351 + P338

Particle Size in Methanol Max

163

pH stability, CIP

2â€“10

3â€“14

2â€“13

2â€“13.7

3â€“12

3â€“10

4â€“13

2â€“9

1â€“14

2â€“14

2â€“14Co²⁺-stripped medium

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

Porosity

7 Î¼m

10 or 80 Âµm

20 Î¼m

10 Î¼m

Pressure Limit

20 bar (2 MPa, 290 psi)

Quantity

1000

1000/Cs.

Each

1000/Pk.

100

500 mL

Quality

monodispersed

with moisture indicator free of heavy metals

dry

No per Pack

100

Preslit

Quantity

100 g

100 Pack

100/Pk.

200

144 Pack

Osmolality

< 25 mOsm/kg

Recommended Flow Rate

1 ml/min

Product Line

Easy Spray

Pierce

Resin Type

MabSelect

Capto

Sepharose Fast Flow

Sepharose High Performance

pH ligand fully charged

Above 6

Entire pH range

Below 9

Pictogram

GHS07

GHS08

Refractive Index

1.4770 to 1.4800 (at 25°C)

Preslit

Yes

S-Codes

26-36

Sample Volume

<= 13 ml

4 to 50 ÂµL

600 Âµl

<= 1.5 ml

< 0.5 mL

< 5 mL

25 to 500 ÂµL

<= 15 ml

<= 5 ml

Scale

Lab|Process

Lab|Process development

Lab

Lab|Process development|Process

Sealing

Heat-fused

Septum

None

ParticleSize

3 µm

5 µm

Recommended maximum operational flow velocity

300 cm/h

pH Stability Cleaning-in-Place (CIP)

1 to 14

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

PurityGrade Notes

Mobile phase for HPLC applications

No per Pack

100

R-CODES

36/37/38

48/20

Particle Shape

Spherical

Particle Size in Methanol Min

PoreSize

300 Å

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stability

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Residueafter Evaporation

5ppm max.

Stationary Phase

C18

Reversed Phase/Anion Exchange/Cation Exchange

Storage

2 to 8Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2-8C

4Â°C to 8Â°C

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

Operating Pressure Max

4.1 bar (60 psi)

Surface Area

250-350 m 2 /g

530 m 2 /g

170 m 2 /g

22 m 2 /g

320 m 2 /g

450 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

~500 m 2 /g

100 m²/g

300 m²/g

500 m 2 /g (approx.)

800 m 2 /g

289 m 2 /g

450 m 2 /g

480 m 2 /g

100 m 2 /g

500 m 2 /g

300 m 2 /g

470-530 m 2 /g

Shelf Life

24 months

Signal Word

Danger

Warning

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Storage Conditions

4 - 30Â°C

4ËšC to 30ËšC, 20% ethanol

At room temperature. Precipitation can occur at low temperature.

4â€“30Â°C

Sterilization

0.1 Âµm sterile filtered

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

Purity

>=99%

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

ProductLine

Acclaim

Support System

Polypropylene (PP)

RTECS Number

VV7315000

Septum

Red PTFE/White Silicone

Stokes Radius

~ 10

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

Swelling factor

~1.7

SMILES

CS(=O)C

Synonym

DMSO

Solubility Information

Solubility aq. soln.: insoluble.

Synonyms

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

Theoretical Plates

> 13000 m-1

> 38 000 m^-1

5000 m-1

> 43 000 m^-1

> 48 000 m^-1

Thread Size

9 mm

10-425

20 mm

Type

Analytical Column

Liquid Chromatography (LC)

HPLC Column

Total binding capacity

~50 mg hIgG/mL resin

USP Type

Viscosity

1.98 mPa/s at 25Â°C

Volume Metric

4 mL

2 mL

Volume Metric Total

2 mL

System

Sepax C-Pro|Sefia|Sepax 2

IUPAC Name

methylsulfinylmethane

VialType

11 mm Crimp Top Vial

9 mm Target DP Vial

Volume

400 Âµl - 2 ml

1 mL

5-20 ml

22 mL

3 mL

50 mL

6 mL

100-500 Âµl

0.5 mL

2-6 ml

2 mL

25 mL

Total Time of Preparation

< 30 min/6 preps

U SPType

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Wetted Part

yes

WGK

nwg

Volume English

1 dr.

Volume_28

100-500 Âµl

400 Âµl - 2 ml

5-20 ml

2-6 ml

>1600 C

Noper Pack

100

Volume Metric

2.0 mL

Water

0.04% max.

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Vent

On inlet

Volume MetricTotal

2 mL

Volume Metric Working

1.8 mL

Temperature

60°C

Titratable Acid

0.001mEq/g max.

Volume MetricUsable

4 mL

1.5 mL

Vapor Density

<1 (vs air)

Viscosity Max

Volume Metric Usable

1.5 mL

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Volume MetricResidual

<170µL

Volume Metric Residual

<170µL

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