Disposable PD-10 Desalting Column, with Sephadex G-25 resin, 1.0–2.5 mL samples
Flexible run protocols – perform desalting and buffer exchange using gravity flow (1.0 to 2.5 mL samples) or centrifugation (1.75 to 2.5 mL samples)
Flexible throughput – desalt one sample or multiple samples in parallel
Good recovery – in the range of 70% to > 95%
High desalting capacity – remove > 98% salt with gravity; > 90% with centrifugation
Minimal dilution – typically none when used as spin columns; 1.4× when used with gravity flow
Time savings compared to dialysis – inexpensive and convenient alternative to lengthy and troublesome dialysis procedures