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Search results for : Liquid chromatography LC columns

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Chromatography

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Chromatograph Columns

Liquid chromatography LC columns

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Application

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

Highly suitable for preparative purification of histidinetagged recombinant proteins

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Application_4

Chromatography system|Manual

Applications

LPLC: suitable

Bed Height With Two Adaptors

85 mm-350 mm

0 to 200 mm

0-280 mm

0 mm-26 mm

158 mm-206 mm

680 mm-950 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

96 mm-156 mm

80 mm-350 mm

58 mm - 106 mm

Bed Support

Filter

Net

Bed Dimensions

16 x 100 mm

16 x 600 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

7 x 25 mm

16 x 25 mm

26 x 600 mm

Bed Height

600 mm

100 mm

25 mm

Bed Volume

1 ml TALON® Superflow /column

5 mL

1 mL

120

320

Avoid Using

Oxidizing agents, citrate buffers

Chelating agents such as EDTA, EGTA , citrate

Bed Height With One Adaptor

579 mm-609 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

225 mm-350 mm

525 mm-650 mm

85 mm-109 mm

0 mm-29 mm

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

125 mg human IgG/column

Binding Capacity/ml Chromatography Medium

~ 13 mg ovalbumin

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

Average sample loading capacity

50 mg

BioProcess resin

yes

Application_5

Chromatography system|Manual

Bore Size (mm)

Capacity

1.1 meq/mL by wetted bed volume

Chemical Stability

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in aqueous buffers commonly used in Protein A Chromatography

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

COLOR

Blue

Column i.d.

5 mm

10 mm

16 mm

50 mm

26 mm

CAS_NO

69011-20-7

9052-95-3

Diameter Metric Outer

32 mm

Certificate of Analysis

Yes

Chemical Substance ID

24870506

Chromatography technique

Ion Exchange Chromatography

Histidine-tagged protein purification

Group specific affinity

Antibody affinity chromatography

GST-tagged protein purification

Hydrophobic Interaction Chromatography

Column Capacity

< 1 mg/mL

66 mL

Column volume

1 mL

Description

Omnifit EZ Replacement Glass, 6.6 x 50mm; 1/ea

Diameter Metric Inner

13 mm

6.6 mm

26 mm

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

0.8-3.9 ml/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

< 1 mL/min

< 15 ml/min

5 ml/min - 20 ml/min

< 20 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

> 20 ml/min|> 20 ml/min

1.6 mL/min

< 2.6 ml/min

1.2 - 2.3

2 ml/min - 10 ml/min

Flow Velocity

< 150 cm/h

Up to 40 cm/h

â‰¤ 600 cm/h

< 300 cm/h

< 500 cm/h|< 500 cm/h

15 cm/h|15 cm/h

15 cm/h

< 300 cm/h|< 300 cm/h

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

< 450 cm/h

< 600 cm/h

For Use With

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

PD-10 Desalting Columns

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

Connect a syringe to the top of a HiTrap columns.

For Use With Equipment

420570 Series reservoir and 420575 series flow control adapters

Omnifit EZ and EZ SolventPlus Columns

Format_6

Prepacked column

Prepacked columns

Kits

Bulk resin

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

For Use With Application

High resolution size exclusion chromatography (SEC) in micro scale (4 to 50μL sample volume)

H-Codes

H319

Hold-up Volume of Membrane

<20 Î¼l

<10 Î¼l

<5 Î¼l

Filtrate Vessel Material

Polycarbonate (PC)

Ligand

Glutathione

Recombinant protein A (E.coli)

Alkali-stabilized Protein A-derived (E. coli)

Octylamine

Ligand Density

6 mg rProtein A/ml medium

Inlet id

1.0 mm (0.04 in)

1.55 mm (0.06 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

2 mm (0.08 in)

IOD-Codes

Length Metric

500 mm

457 mm

50 mm

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

Includes

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Instructions supplied with the concentractors

Operating Temperature

4Â°C-60Â°C

Order Information

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

HiLoad columns are prepacked XK columns

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Pack size

1 Ã— 120 mL

1 piece

20 mL

1 Ã— 5 mL

50 Ã— 100 ÂµL

50 columns

7 Ã— 1 mL

53 mL

100 mL

1 kit

5 Ã— 1 mL

200 mL

25 mL

4 x 53 mL

1 Ã— 320 mL

4.7 mL

5 Ã— 5 mL

20 columns

30 columns

5 mL

Ion exchanger

S (cation exchanger)

pH stability, operational

3â€“12

3â€“10

3â€“13

4â€“9

Porosity

10 Î¼m

7 Î¼m

20 Î¼m

10 or 80 Âµm

Ligand_7

2-mercaptopyridine

Protein A, alkali stablilized

Protein A

Protein A, alkali stabilized

Ionic Capacity

40-85 Âµmol/ml (Cl^-)

Sample Volume

<= 15 ml

<= 5 ml

<= 13 mL

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

2.5 ml

600 Âµl

< 0.5 mL

< 5 mL

<= 1.5 ml

100-180 Î¼l

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Matrix

6% cross-linked agarose

Highly cross-linked agarose, spherical

Highly cross-linked agarose

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

cross-linked agarose, 4%, spherical

Includes

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

Length English

18 in.

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Storage

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

+4Â°C to +8Â°C

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4Â°C to 30Â°C, 20% Ethanol

4 to 30Â°C, in 20% Ethanol

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

Storage Conditions

Store at 2Â°C to 8Â°C

4 - 30Â°C

Material

Polycarbonate (PC)

Stainless Steel

Polypropylene (PP)

Glass

Pack Size

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

MDL Number

MFCD00146442

MFCD00132726

Operating Flow Rate, Recommended

0.5 mL/min

1.3 mL/min

Outlet id

1.0 mm (0.04 in)

1.55 mm (0.06 in)

0.75 mm (0.029 in)

pH stability, CIP

2â€“13.7

3â€“12

2â€“14

2â€“10.5

Pressure Limit

20 bar (2 MPa, 290 psi)

Resin Type

MabSelect

Sepharose Fast Flow

Sepharose High Performance

Molecular Weight

0.0

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Pore Size

40 to 60 µm

P-CODES

P305 + P351 + P338

Particle size, d_50V

~34 Âµm

~34 Î¼m

~85 Âµm

~90 Âµm

Pictogram

GHS07

S-Codes

pH Stability Working Range

3â€“12

Pressure/Flow Specification

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Type

Gravity Chromatography Column

Chromatography Column

Liquid Chromatography (LC)

Particle Size

60 Âµm-165 Âµm

45 Âµm-165 Âµm

Volume Metric Bed

≈2.4mL

RIDADR

NONH for all modes of transport

Theoretical Plates

> 13000 m-1

5000 m-1

PoreSize

40 to 60 µm

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Operating Flow Rate, Max

1.0 mL/min

2.6 mL/min

Volume_28

5-20 ml

2-6 ml

400 Âµl - 2 ml

100-500 Âµl

WGK

Signal Word

Warning

Quantity

Recommended Flow Rate

1 ml/min

R-CODES

36/37/38

Size MetricStopcock

2 mm bore

Volume

5-20 ml

2-6 ml

400 Âµl - 2 ml

100-500 Âµl

Storage Requirements

4° to 30°C

Wetted Part

yes

Volume MetricBed

≈2.4mL

Total binding capacity

~50 mg hIgG/mL resin

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

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