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Chromatography

Chromatography Consumables

Chromatograph Columns

Liquid chromatography LC columns

High pressure liquid chromatography HPLC columns

Ion Chromatography IC Columns

Gas Chromatography GC columns

$0~$10(36)
$10~$25(27)
$25~$50(51)
$50~$75(59)
$75~$100(93)
$100~$200(191)
$200~$300(172)
$300~$400(112)
$400~$500(143)
$500~$750(439)
$750~$1000(503)
$1000~$5000(800)
$5000~$10000(24)
$10000~$50000(2)
$50000~$100000(0)
$100000~$3000000(0)

Temperature

60°C

70°C

50°C

45°C

80°C

Application

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Highly suitable for preparative purification of histidinetagged recombinant proteins

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Application_4

Chromatography system|Manual

Centrifugation

Applications

LPLC: suitable

UHPLC: suitable

gas chromatography (GC): suitable

HPLC: suitable

Avoid Using

Oxidizing agents, citrate buffers

Chelating agents such as EDTA, EGTA , citrate

Application_5

Chromatography system|Manual

Bed Dimensions

3.2 x 300 mm

16 x 25 mm

26 x 600 mm

7 x 25 mm

26 mm x 400 mm

16 x 100 mm

16 x 600 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

Bed Height With Two Adaptors

0 mm-26 mm

158 mm-206 mm

85 mm-350 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

80 mm-350 mm

96 mm-156 mm

58 mm - 106 mm

0 to 200 mm

0-280 mm

680 mm-950 mm

Bed Support

Filter

Frit

Net

Bed Height

25 mm

100 mm

600 mm

300 mm

Bed Volume

~ 2.4 mL

1 mL

320

120

1 ml TALON® Superflow /column

5 mL

212 mL

Average sample loading capacity

50 mg

Bed Height With One Adaptor

85 mm-109 mm

225 mm-350 mm

525 mm-650 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

0 mm-29 mm

579 mm-609 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

Binding Capacity/ml Chromatography Medium

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

Up to 20 mg histidine-tagged protein

~ 13 mg ovalbumin

Capacity

1.1 meq/mL by wetted bed volume

=25 % absorption capacity (water)

3.0 meq/g by dry weight

1.7 meq/mL by wetted bed volume

4.7 meq/g by dry weight

CarbonLoad

15%

11%

18%

20%

Boiling Point

2230 C

Bore Size (mm)

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

125 mg human IgG/column

CAS_NO

112926-00-8

9052-95-3

69011-20-7

39389-20-3

Chemical Name or Material

Silica Gel

Chemical Stability

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Conditions where the resin may be operated without significant change in function:
in¢ Trifluoroacetic acid, up to 0.2%
in¢ Isopropanol, up to 5%
in¢ Dithiothreitol (DTT), up to 5 mM
in¢ Acetonitrile, up to 70%
in¢ Formic acid, up to 1

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid.

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Stable in aqueous buffers commonly used in Protein A Chromatography

Chromatography technique

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Multimodal ion exchange chromatography

Antibody affinity chromatography

GST-tagged protein purification

Group specific affinity

Histidine-tagged protein purification

Column Type

capillary chiral

Specialty/Application Specific

capillary polar

capillary non-polar

packed GC

capillary intermediate polar

Mixed Mode

capillary extremely polar

Reversed Phase

CAS Registry Number 1

7631-86-9

Diameter Metric

2.1 mm

4.6 mm

0.075 mm

BioProcess resin

yes

Endcapped

Proprietary

Yes

Polar

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

< 15 ml/min

< 15 ml/min|< 15 ml/min

5 mL/min - 20 mL/min

> 20 ml/min|> 20 ml/min

1.6 mL/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 20 ml/min|< 20 ml/min

1 mL/min

1 mL/min - 4 mL/min

300 nL/min

0.25 to 0.75 mL/min

0.8-3.9 ml/min

< 20 mL/min

4 mL/min (1 mL), 5 mL/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 1 mL/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 2.6 mL/min

1.2 - 2.3

2 ml/min - 10 ml/min

For Use With Equipment

420570 Series reservoir and 420575 series flow control adapters

Omnifit EZ and EZ SolventPlus Columns

For use with_31

AKTA go protein purification system

Format_6

Prepacked column

Prepacked columns

Bulk resin

Kits

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Certificate of Analysis

Yes

Chemical Substance ID

24870506

329750331

Chemical/Physical Resistance

Temp. Limits:. -10 C to 180 C isothermal and programmed

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Color

Gold/Brown

Gold

Silver

Brass

Black

Column Capacity

66 mL

7.2 to 12.7 mL

< 1 mg/mL

Column i.d.

3.2

10 mm

16 mm

50 mm

26 mm

5 mm

Column Format

Analytical Column

NanoViper Column

Analytical Column, EASY-Spray

Density

1.01 g/mL at 25 °C (true wet)(lit.)

Coating

Fused Silica

Siltek

Diameter Metric

0.25 mm

75 µm

0.32 mm

5.00 µm

0.12 µm

0.50 µm

0.20 µm

0.25 µm

Diameter Metric Inner

0.25 mm

0.32 mm

13 mm

6.6 mm

26 mm

0.53 mm

Description

Omnifit EZ Replacement Glass, 6.6 x 50mm; 1/ea

Omnifit EZ Chromatography Column w/ 2 Adjustable Endpieces, 6.6 x 400mm; 1/ea

1 um Thickness; 0.32 mm ID; 60 m Length

EC Number

215-185-5

231-545-4

262-373-8

200-838-9

231-153-3

Column volume

1 mL

Diameter

1/2 in.

COLOR

Blue

For Use With

C 10 columns

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

AKTA lab protein purification system such as AKTA go, AKTA pure and AKTA avant

Connect a syringe to the top of a HiTrap columns.

PD-10 Desalting Columns

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

Flow Velocity

< 450 cm/h

â‰¤ 600 cm/h

< 300 cm/h

< 500 cm/h|< 500 cm/h

< 150 cm/h

Up to 40 cm/h

< 300 cm/h|< 300 cm/h

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

15 cm/h|15 cm/h

15 cm/h

< 600 cm/h

For Use With

LC/MS, ESI-MS

ELSD, UV Detector

Anilines, Barbiturates, Carbonyls, Fat Soluble Vitamins, Fatty Acids, Glycerides, Phthalates, PTH Amino Acids, Steroids, Other Acids

HPLC

Feature

Not Endcapped

swivel design

preconditioned

unconditioned

biocompatible

Endcapped

Film Thickness

5 µm

1 µm

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

Service & Support

For Use With Application

Aromatics Analysis

High resolution size exclusion chromatography (SEC) in micro scale (4 to 50μL sample volume)

GC/MS for Hydrocarbons, Solvent Impurities, PCB Congeners, and Aroclor Mixes

Crossbond

FilmThickness

3 µm

1 µm

Diameter Metric Outer

32 mm

Formula Weight

60.08

Dimensions

H x W x D: 660 x 860 x 710

Functionality InteractionType

Van der Waals interaction

Filtrate Vessel Material

Polycarbonate (PC)

H-Codes

H351

H315-H319-H335

H317

H319

Guidance

For AKTAprime

Hold-up Volume of Membrane

<20 Î¼l

<10 Î¼l

<5 Î¼l

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

2 mm (0.08 in)

Includes

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

3.5 in. Coil

IOD-Codes

Ion exchanger

S (cation exchanger)

Ligand

Octylamine

Alkali-stabilized Protein A-derived (E. coli)

TALONÂ® Superflow Cobalt-IMAC

Recombinant protein A (E.coli)

Glutathione

Length Metric

60 m

500 mm

400 mm

457 mm

100 mm

250 mm

50 mm

Length Metric

750 mm

150 mm

30 m

20 mm

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

cross-linked agarose, 4%, spherical

Highly cross-linked agarose, spherical

Highly cross-linked agarose

Composite of cross-linked agarose and dextran

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Material

Fused silica

Silica Gel

Stainless Steel

stainless steel hardware

stainless steel hardware (column)

Metal

stainless steel column

stainless steel holder

Polypropylene (PP)

stainless steel TD tube

Plastic/Stainless Steel

Glass

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

glass TD tube

Polycarbonate (PC)

stainless steel frit

Glutathione Sepharose 4 B

Mixed

Acetal plastic (POM)

N/A

Ligand_7

Protein L

Protein G

Protein A, alkali stablilized

2-mercaptopyridine

Protein A

Protein A, alkali stabilized

Max Temperature

340/360°C

40°C

330/350°C for Standard Columns; 430°C for Metal Columns

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

Material

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

100% Dimethyl Polysiloxane

Max Pressure

4000 psi (275 bar)

14500 psi (1000 bar)

900 psi

5800 psi (400 bar)

11600 psi (800 bar)

17400 psi (1200 bar)

4500 psi (310 bar)

Ionic Capacity

40-85 Âµmol/ml (Cl^-)

MDL Number

MFCD00011232

MFCD00146442

MFCD00132726

MFCD00145841

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Matrix

6% Cross-Linked Agarose

Length English

18 in.

Ligand Type

Monomeric C18

Max Temperature

445°C

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Operating Flow Rate, Max

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Operating pH

0 - 9

3.8 - 6.8

0 - 14

2 - 7.5

3.5 - 7.0

1 - 8

2 - 8

1 - 9

2 - 9

Pack size

50 mL

4.7 mL

5 Ã— 5 mL

1 Ã— 5 mL

50 Ã— 100 ÂµL

50 columns

7 Ã— 1 mL

20 columns

30 columns

5 mL

250 columns

53 mL

100 mL

1 Ã— 320 mL

10 mL

200 mL

25 mL

4 x 53 mL

1 kit

10 Ã— 2 mL

5 Ã— 1 mL

1 Ã— 120 mL

1 piece

20 mL

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Nanopolymer Hybrid Silica

Spherical, Fully Porous, Base-Deactivated Silica

Silica

Spherical, Solid Core, Ultrapure Silica

Type B Silica

Particle Size

3 µm

45 Âµm-165 Âµm

90 µm

60 Âµm-165 Âµm

40 to 60 µm

60 Î¼m-160 Î¼m

2 µm

Particle size, d_50V

~90 Âµm

~ 9 Âµm

~34 Âµm

~34 Î¼m

~85 Âµm

Order Information

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

HiLoad columns are prepacked XK columns

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Outer Diameter

12 or 18 mm

10-21 mm

Pore Size

110 Å pore diameter

2 µm porosity

~0.65 mL/g pore volume

160 Å

300 Å

40 to 60 µm

90 Å pore size

100 Å

120 Å

90 Å

180 Å

~1.30 mL/g pore volume

60 +?

1000 Å

100 Å

PackingMaterial

Forte BP1

Mxt-502.2

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Phase

Reversed Phase and Ion Exchange

Reversed Phase

Operating Flow Rate, Recommended

0.075 mL/min

0.5 mL/min

1.3 mL/min

0.45 mL/min

Polarity

Non polar

Non-Polar

p H

4 to 13

2.5 to 8

2 to 9

2 to 8

2.5 to 7.5

2.5 to 7

Pack Size

Outlet id

0.75 mm (0.029 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Packaging Layout

1, 6x1, 2.5 kg in poly bottle

1 ft3 in fiber drum

ParticleSize

2.6 µm

Monolith

3 µm

90 µm

2.2 µm

5 µm

pH stability, operational

3â€“10

4â€“9

3 to 12

3â€“13

3â€“12

P-CODES

P280

P305 + P351 + P338

ProductLine

TraceGOLD

Accucore

Hypersil BDS

Acclaim

Packaging

Plastic Drum

Particle Shape

Spherical Solid Core

Spherical

pH stability, CIP

2â€“13.7

3â€“12

2â€“10.5

2â€“14

2â€“14Co²⁺-stripped medium

Porosity

7 Î¼m

10 or 80 Âµm

20 Î¼m

10 Î¼m

Pressure Limit

20 bar (2 MPa, 290 psi)

2 MPa

PoreSize

130 Å (Mesopore Size), 2 µm (Macropore Size)

80 Å

120 Å

130 Å

300 Å

40 to 60 µm

100 Å

10 µm and 30 µm

Quality

with moisture indicator free of heavy metals

dry

Power Consumption

8 VA

Operating Temperature

4Â°C-35Â°C

4Â°C-60Â°C

Quantity

100 g

10 g

10 mL

30 kg

100 mL

Recommended Flow Rate

1 ml/min

Product Line

Easy Spray

nanoLC

TraceGOLD

Resin Type

MabSelect

Capto

Sepharose Fast Flow

Sepharose High Performance

Superdex 200 Increase

Quantity

5 kg

1/Pk.

Pictogram

GHS07

GHS08

Pressure/Flow Specification

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Molecular Formula

O2Si

Sample Volume

<= 13 ml

4 to 50 ÂµL

< 150 Âµl

100-180 Î¼l

600 Âµl

<= 1.5 ml

< 0.5 mL

< 5 mL

2.5 ml

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

< 100 Âµl

<= 15 ml

<= 5 ml

Up to 8.5 mL

Molecular Weight

0.0

Scale

Lab|Process

Lab|Process development

Lab

Lab|Process development|Process

pH Stability Cleaning-in-Place (CIP)

1 to 14

RIDADR

NONH for all modes of transport

R-CODES

36/37/38

45-36/38

Relative Humidity

20%-95% (non condensing)

S-Codes

53.1-26-45

26-36

pH Stability Working Range

3â€“12

Stationary Phase

C18

Proprietary

Reversed Phase/Anion Exchange/Cation Exchange

TraceGOLD™ TG-1 MS

Storage

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

4 to 30Â°C, in 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

+4Â°C to +8Â°C

20 to 25Â°C, Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative

20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4Â°C to 30Â°C, 20% ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

4 to 30Â°C, 20% Ethanol (Q, DEAE), 20% Ethanol, 0.2 M Sodium Acetate (S)

Surface Area

~300 m 2 /g

170 m²/g

170 m 2 /g

22 m 2 /g

320 m 2 /g

130 m²/g

450 m 2 /g

200 m 2 /g

80 m 2 /g

450 m²/g

~500 m 2 /g

100 m²/g

300 m²/g

450 m 2 /g

100 m 2 /g

300 m 2 /g

Storage Conditions

4 - 30Â°C

4ËšC to 30ËšC, 20% ethanol

Store at 2Â°C to 8Â°C

Signal Word

Warning

Size MetricStopcock

2 mm bore

StationaryPhase

100% Dimethyl Polysiloxane

TraceGOLD TG-1 MS

Proprietary Diphenyl Dimethyl Polysiloxane

Storage Requirements

4° to 30°C

Temperature MetricRange

4 to 40°C

Solubility Information

Solubility aq. soln.: insoluble.

Technique

HPLC

Theoretical Plates

> 13000 m-1

> 38 000 m^-1

5000 m-1

Type

Analytical Column

Liquid Chromatography (LC)

Chromatography Column

HPLC Column

Gravity Chromatography Column

Total binding capacity

~50 mg hIgG/mL resin

USP Type

USPType

G1, G2, G38

Volume

400 Âµl - 2 ml

5-20 ml

100-500 Âµl

2-6 ml

U SPType

Wetted Part

yes

Ligand Density

6 mg rProtein A/ml medium

Linear Formula

(C10H12 · C10H10 · C8H8)x

Synonyms

SUPELCOGEL(TM) 8% Crosslinked HPLC Column

xad-2

Silica gel

Volume_28

100-500 Âµl

400 Âµl - 2 ml

5-20 ml

2-6 ml

Temperature Range

-60°C to +340/360°C

-20°C to +270/320°C

>1600 C

Type

BP1 GC Column

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Wetted materials1

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Temperature

60°C

Wetted Materials

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

WGK

nwg

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Volume MetricBed

≈2.4mL

Volume Metric Bed

≈2.4mL

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