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Chromatography

Chromatography Consumables

Reagents and Buffers

Chromatography Media

$0~$10(0)
$10~$25(0)
$25~$50(182)
$50~$75(52)
$75~$100(17)
$100~$200(73)
$200~$300(53)
$300~$400(21)
$400~$500(27)
$500~$750(40)
$750~$1000(29)
$1000~$5000(99)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Application_4

Chromatography system|Manual

Chromatography system

Cell isolation

Bed Volume

5 mL

1 mL

320

Binding Capacity/ml Chromatography Medium

> 25 mg horse liver GST/mL resin

At least 10 mg histidine-tagged protein

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

Capacity

1.35 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

4.7 meq/g by dry weight

0.8 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

Average sample loading capacity

400 mg

50 mg

Bed Dimensions

16 x 100 mm

16 x 30 mm

7 x 25 mm

16 x 25 mm

26 x 600 mm

10 x 100 mm

6.4 x 100 mm

Bead type

Mag Sepharose Magnetic Beads

Bed Height

600 mm

100 mm

25 mm

30 mm

Autoignition Temp

800 °F

~800 °F

Applications

ion chromatography: suitable

Assay

=99%

99.8%

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

At least 10 mg histidine-tagged protein/ml sedimented medium.

BioProcess resin

yes

Boiling Point

189°C

2230 C

Application_5

Chromatography system|Manual

CAS_NO

11128-95-3

126850-14-4

1344-28-1

69011-19-4

112945-52-5

69011-20-7

39339-85-0

7631-86-9

112926-00-8

1343-88-0

104219-63-8

60177-39-1

62610-50-8

65099-79-8

69011-15-0

9004-54-0

39389-20-3

9003-70-7

9041-35-4

9050-97-9

63181-98-6

65546-95-4

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

CAS Registry Number 1

67-68-5

Assay Percent Range

99.8% min by GC

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Chemical Stability

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in all commonly used aqueous buffers

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Chemical Substance ID

24854187

329756032

24852837

24854823

329759183

329759193

329768201

24852120

24854190

24857354

24867159

24863799

24865203

24854645

24857358

329759188

329759187

329759181

329768868

329763462

24854191

329752189

24854194

24857352

329752001

329763461

COLOR

Blue

Density Max.

1.078 g/ml

1.2 g/ml

1.135 g/ml|

Chem AlertStorageSymbol

Gray

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Stable in commonly used aqueous buffers, 1 M NaOH

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

ChEBI

CHEBI:28262

Chromatography technique

Ion Exchange Chromatography

Histidine-tagged protein purification

Antibody affinity chromatography.

Group specific affinity

Antibody affinity chromatography

GST-tagged protein purification

Affinity coupling

Hydrophobic Interaction Chromatography

Chemical Name or Material

Dimethylsulfoxide

Column i.d.

6.4

16 mm

26 mm

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Concentration

1 N in NaOH (Regenerant concentration)

Color

White

Column volume

1 mL

8 mL

Color

Colorless to slightly yellow

Gold/Brown

Colorless

Decomposition Information

>190°C

Density

1.100g/cmÂ³

Composition

nitrogen content, =1.0%

EC Number

215-681-1

238-878-4

231-545-4

215-691-6

Endotoxin Activity Max.

2 EU/ml

< 0.12 EU/ml

0.12 EU/mg

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 5 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 20 mL/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 ml/min

> 4 ml/min

< 20 mL/min |< 20 mL/min

1-4ml/min

2 ml/min - 10 ml/min

Flow Velocity

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 150 cm/h

<15 cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 500 cm/h

30-150cm/h

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

‰¤ 600 cm/h|‰¤ 600 cm/h

Exclusion Limit Peptides

4 x 10Â³

Empirical Formula

MgO3Si

O2Si

Estimated Shelf Life from Manufacture Date

3 years

5 years

8 years

Feature

ferrule type packed column

Flash Point

87°C (188.6°F)

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Format_6

Prepacked column

Prepacked columns

Bulk resin

Format

matrix material

Formula

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Al2O3

SiO₂

Service & Support

MgO₃Si

O2Si

Form

Powder

powder or crystals

beads

fine crystals

fine powder

Flashpoint (°C)

> 240 C

Formula Weight

100.39

60.08

78.13

Grade

Certified Reference Material

technical grade

high-purity grade (Davisil Grade 646)

high-purity grade (Davisil Grade 643)

HPLC

high-purity grade (Davisil Grade 633)

high-purity grade (Davisil Grade 923)

Standard

Grade

High-purity Grade (Davisil Grade 636)

Nuclear Grade

For column chromatography

High-purity Grade

High-purity Grade (Davisil Grade 62)

High-purity Grade (7754)

Group To be Coupled

Primary amines

-NHâ‚‚

H-Codes

H319

H373

H372

H371

Identification

Pass Test

Health Hazard 2

WARNING!

Ion Exchanger Type

Weak cation exchanger

Strong anion exchanger

Weak anion

Weak anion exchanger

Weak and strong anion

Multimodal weak cation exchanger

Strong cation exchanger

Ionic Capacity

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.29-0.35 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.15-0.20 mmol H⁺/mlÂ

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.13-0.17 mmol/ml

IUPAC Name

methylsulfinylmethane

Ligand

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Cibacron Blue

Human IgG

Quaternary amine

Carboxymethyl

Sulphopropyl

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Diethylaminoethyl

Porcine heparin

Recombinant protein A (E.coli)

Native Protein A

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Alkali-stabilized Protein A-derived (E. coli)

Alkaline-stabilized protein A-derived (E.coli).

Ligand Concentration

~4mg heparin/mL resin

~25 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~45 Âµmol phenyl/mL resin

Ligand Coupling Method

Reductive Amination

Epoxy activation

Epoxy

Maleimide activation

Ether linkages and hydrophilic spacer arm

Ether linkages

Cyanogen bromide activation

Triazine

Dextran surface extenders

Linear Formula

(C10H12 · C10H10 · C8H8)x

SiO2

MgO3Si

(C10H12 · C10H10 · C8H8 · C3H9N)X

(CH₃)₂SO

Material

N/A

Matrix

2% agarose

Cross-linked dextran

agarose, 6%, spherical

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Highly cross-linked 6% agarose

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

cross-linked, 6% agarose particles

Highly cross-linked agarose

Cross-linked agarose, 4%

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Rigid, cross-linked agarose, spherical

MDL Number

MFCD00145841

MFCD00011232

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00211881

MFCD00132722

MFCD00132707

MFCD00132771

MFCD00132712

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00145586

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00084428

MFCD03791384

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

Intrinsic Viscosity

~ 17

IOD-Codes

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Includes

Connectors for high- and low-pressure chromatography equipment and instructions.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand_7

Butyl, octyl, phenyl

Protein L

Protein G

Protein A

Protein A, alkali stabilized

Molecular Weight

100.39

60.08

0.0

101.96

78.129

60.08

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

>1600 C

Operating Flow Rate, Recommended

0.30 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.5 mL/min

0.50 mL/min

1.3 mL/min

0.75 mL/min

Melting Point

18.4°C

Pack size

20 mL

300 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

500 mL

1.7 mL

100 mL

125 mL

25 g

1.5 g

5 Ã— 1 mL

6 x 1 L

1 L

10 mL

200 mL

25 mL

500 g

6 x 500 mL

15 g

2 x 1 ml 10% slurry

50 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

100 g

5 mL

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

pH stability, operational

3â€“12

3â€“9

3â€“10

2â€“12

3 to 12

3â€“11

3â€“13

2â€“9

4â€“13

4â€“9

2â€“10

pH 6 to 10

2â€“13

4â€“12

Pore Size

150 Å

90 Å mean pore size

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

0.7-0.85 cm 3 /g pore volume (approx.)

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

110 Å pore size

60 Å mean pore size

0.75 cm 3 /g pore volume

~0.55 mL/g pore volume

~0.8 cm 3 /g pore volume

Pressure/flow characteristics

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/Flow Specification

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

R-CODES

48/20

36/37/38

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Sample Volume

<= 5 ml

100 to 300 Î¼l

<= 13 mL

4 to 50 ÂµL

25 to 500 ÂµL

400 to 1000 Î¼l

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Ligand Density

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Approx. 13 Î¼mol/ml

~ 5 mg/mL resin

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

Material

Plastic/Stainless Steel

Stability

Stable

Storage

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2-8C

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 100% Isopropanol

Surface Area

300 m 2 /g

470-530 m 2 /g

250-350 m 2 /g

530 m 2 /g

289 m 2 /g

480 m 2 /g

175-225 m 2 /g

380 m 2 /g

500 m 2 /g

~30 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

~500 m 2 /g

550 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

Pack Size

Packaging

Amber Glass

Packaging Layout

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

500 g in poly drum

2.5 kg in poly drum

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

100, 500 g in poly bottle

5, 25 kg in fiber drum

2.5, 5, 25 kg in fiber drum

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

Percent Purity

≥99.9%

pH Range

5.5â€“7.5

8.5â€“9.5

8.9â€“9.9

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

Physical State

liquidSuspension

Liquid

solid

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Matrix

Highly cross-linked agarose, spherical.

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Operating Flow Rate, Max

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

Optical Activity

7.0 (5% in slurry)

6.5 (5% in slurry)

~7 (5% in H 2 O, suspension)

Order Information

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Percoll PLUS is for research use only.

For research use only.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

HiLoad columns are prepacked XK columns.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Osmolality

288-310 mOsm/kg

< 25 mOsm/kg

276-298 mOsm/kg

322-344 mOsm/kg

< 30 mOsm/kg

P-CODES

P260

P314

P305 + P351 + P338

Particle Size

60 Âµm

24-44 Âµm

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

60 Î¼m-200 Î¼m

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

Particle size, d_50V

~90 Î¼m

~ 8.6 Âµm

~50 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~85 Âµm

~90 Âµm

34 Âµm

~75 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

pH stability, CIP

2â€“13

2â€“13.7

3â€“12

3â€“13

2â€“12

1â€“14

2â€“14

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

3â€“10

4â€“13

2â€“10

3â€“14

Quality

monodispersed

dry

wet

regenerated

Quantity

100 g

50 g

500 g

Resin Type

MabSelect

Sepharose CL-4B

Capto

Sepharose Fast Flow

Sepharose High Performance

Synonyms

Activated magnesium silicate; Magnesium silicate

Silica gel

Silica gel 110 NH2

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Amberlite(R) IR120 hydrogen form

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

RTECS Number

VV7310000

VV7315000

Stability

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Particle size distribution, wet

15â€“88 Âµm

90â€“280 Âµm

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

Residueafter Evaporation

5ppm max.

Operating pH

0 - 7

0 - 14

Signal Word

Danger

Warning

Partical Size Dry

50 Âµm-150 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>20 Âµm

18 Âµm-111 Âµm

>100 Âµm

pH ligand fully charged

Below 9

Above 6

< 9

Entire pH range

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Particle Size in Methanol Min

Pictogram

GHS07

GHS08

S-Codes

26-36

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Molecular Formula

C2H6OS

Stokes Radius

~ 10

pH Stability Cleaning-in-Place (CIP)

1 to 14

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Theoretical Plates

> 13000 m-1

> 42 000 m^-1

> 48 000 m^-1

5000 m-1

> 43 000 m^-1

Titratable Acid

0.001mEq/g max.

Vapor Density

>1 (vs air)

<1 (vs air)

Viscosity

1.98 mPa/s at 25Â°C

WGK

nwg

Synonym

DMSO

Storage Conditions

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

20% ethanol at 4Â°C to 30Â°C

Purity

99.8%

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Purity

>=99%

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Recommended Flow Rate

5 ml/min

1 ml/min

Pub Chem CID

679

Refractive Index

1.4770 to 1.4800 (at 25°C)

Purity GradeNotes

Mobile phase for HPLC applications

PurityGrade Notes

Mobile phase for HPLC applications

SMILES

CS(=O)C

Swelling factor

~1.7

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Type of ion exchanger

Strong anion exchanger (quaternary ammonium)

Strong cation exchanger (methyl sulfonate)

System

Sepax C-Pro|Sefia|Sepax 2

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Water

0.04% max.

Viscosity Max

Wetted Part

Well Volume

8.5

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