HisTrap FF 5 X 5 mL
High binding capacity, approx. 4NA mg/ml medium.
Negligible leakage of Ni2+.
Prepacked columns offer reliable and convenient timesaving purification of histidinetagged recombinant proteins.
Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.
Ni Sepharose 6 Fast Flow consists of 9NA µm beads of highly crosslinked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni2+ ions, the firstchoice metal ion for purifying most histidinetagged proteins. The negligible leakage of Ni2+ ions from the matrix ensures reliable capture of histidinetagged proteins in repeated IMAC purifications
The high flow rates made possible by the Ni Sepharose 6 Fast Flow matrix make HisTrap FF columns well suited for small scaleup. Capacity can be increased by connecting columns in series. HiTrap IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.