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Search results for : Liquid chromatography LC columns : "Stratus "

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Liquid chromatography LC columns

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Application

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Highly suitable for preparative purification of histidinetagged recombinant proteins

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Application_4

Chromatography system|Manual

Applications

LPLC: suitable

Bed Height

600 mm

100 mm

25 mm

Bed Volume

1 ml TALON® Superflow /column

5 mL

320

120

1 mL

Bed Height With Two Adaptors

85 mm-350 mm

680 mm-950 mm

0 mm-26 mm

158 mm-206 mm

80 mm-350 mm

0 to 200 mm

0-280 mm

58 mm - 106 mm

96 mm-156 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

Bed Support

Filter

Net

Bed Dimensions

0.7 x 2.5 cm

1.6 x 2.5 cm

16 x 25 mm

26 x 600 mm

16 x 100 mm

16 x 600 mm

7 x 25 mm

Bed Height With One Adaptor

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

85 mm-109 mm

579 mm-609 mm

225 mm-350 mm

525 mm-650 mm

0 mm-29 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

125 mg human IgG/column

up to 15mg histidine-tagged protein

Binding Capacity/ml Chromatography Medium

~ 13 mg ovalbumin

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

Avoid Using

Oxidizing agents, citrate buffers

Chelating agents such as EDTA, EGTA , citrate

BioProcess resin

yes

Average sample loading capacity

50 mg

Bore Size (mm)

Capacity

1.1 meq/mL by wetted bed volume

CAS_NO

69011-20-7

9052-95-3

Certificate of Analysis

Yes

Chemical Stability

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Stable in aqueous buffers commonly used in Protein A Chromatography

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Substance ID

24870506

Chromatography technique

Antibody affinity chromatography

GST-tagged protein purification

Ion Exchange Chromatography

Histidine-tagged protein purification

Hydrophobic Interaction Chromatography

Group specific affinity

Column i.d.

50 mm

10 mm

16 mm

26 mm

5 mm

Column Capacity

0.1 to 4.1 mL

< 1 mg/mL

66 mL

Application_5

Chromatography system|Manual

Column volume

1 mL

Description

Omnifit EZ Replacement Glass, 6.6 x 50mm; 1/ea

Omnifit EZ Chromatography Column w/ 2 Adjustable Endpieces, 6.6 x 150mm; 1/ea

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

< 20 mL/min

4 mL/min (1 mL), 5 mL/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 15 ml/min

5 ml/min - 20 ml/min

0.8-3.9 ml/min

< 2.6 mL/min

1.2 - 2.3

2 ml/min - 10 ml/min

< 1 ml/min

> 20 ml/min|> 20 ml/min

1.6 mL/min

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 4 ml/min

< 4 ml/min|< 4 ml/min

COLOR

Blue

Flow Velocity

< 300 cm/h|< 300 cm/h

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

15 cm/h

< 150 cm/h

Up to 40 cm/h

< 600 cm/h

15 cm/h|15 cm/h

< 450 cm/h

< 300 cm/h

< 500 cm/h|< 500 cm/h

â‰¤ 600 cm/h

For Use With Equipment

420570 Series reservoir and 420575 series flow control adapters

Omnifit EZ and EZ SolventPlus Columns

For Use With

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

Connect a syringe to the top of a HiTrap columns.

PD-10 Desalting Columns

Format_6

Prepacked column

Kits

Bulk resin

Prepacked columns

Diameter Metric Outer

32 mm

Diameter Metric Inner

13 mm

6.6 mm

26 mm

Filtrate Vessel Material

Polycarbonate (PC)

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

For Use With Application

High resolution size exclusion chromatography (SEC) in micro scale (4 to 50μL sample volume)

Hold-up Volume of Membrane

<5 Î¼l

<10 Î¼l

<20 Î¼l

H-Codes

H319

Inlet id

2 mm (0.08 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Length Metric

50 mm

Ligand_7

Protein A

Protein A, alkali stabilized

Protein A, alkali stablilized

2-mercaptopyridine

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

Length Metric

150 mm

500 mm

457 mm

MDL Number

MFCD00132726

MFCD00146442

Matrix

6% Cross-Linked Agarose

Highly crossed-linked agarose, 6%

Matrix

Highly cross-linked agarose, spherical

Highly cross-linked agarose, 6%

Highly cross-linked agarose

Cross-linked agarose, 4%, spherical

Ligand

Glutathione

Octylamine

Recombinant protein A (E.coli)

Alkali-stabilized Protein A-derived (E. coli)

Ligand Density

6 mg rProtein A/ml medium

Max Pressure

900 psi

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Ionic Capacity

40-85 Âµmol/ml (Cl^-)

Length English

18 in.

Material

Polycarbonate (PC)

Glass

Stainless Steel

Polypropylene (PP)

Particle size, d_50V

~90 Âµm

~34 Âµm

~34 Î¼m

~85 Âµm

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Storage

+4Â°C to +8Â°C

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4Â°C to 30Â°C, 20% Ethanol

4 to 30ËšC

4Â°C to 8Â°C

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

4 to 30Â°C, in 20% ethanol

Order Information

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

HiLoad columns are prepacked XK columns

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

Outlet id

1.55 mm (0.06 in)

1.0 mm (0.04 in)

0.75 mm (0.029 in)

Pack size

1 Ã— 320 mL

1 Ã— 5 mL

50 Ã— 100 ÂµL

50 columns

7 Ã— 1 mL

1 Ã— 120 mL

1 piece

20 mL

200 mL

25 mL

4 x 53 mL

53 mL

100 mL

4.7 mL

5 Ã— 5 mL

20 columns

30 columns

5 mL

1 kit

5 Ã— 1 mL

pH stability, CIP

2â€“10.5

2â€“14

2â€“13.7

3â€“12

Porosity

10 or 80 Âµm

10 Î¼m

20 Î¼m

7 Î¼m

Quantity

Resin Type

Sepharose High Performance

MabSelect

Sepharose Fast Flow

Sample Volume

<= 1.5 ml

2.5 ml

<= 13 ml

<= 15 ml

<= 5 ml

< 0.5 mL

< 5 mL

600 Âµl

100-180 Î¼l

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

Size MetricStopcock

2 mm bore

IOD-Codes

Pictogram

GHS07

Pressure/Flow Specification

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Storage Requirements

4° to 30°C

Volume_28

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

5-20 ml

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Pack Size

Molecular Weight

0.0

pH stability, operational

3â€“10

3â€“12

4â€“9

3â€“13

Max Temperature

40°C

Pore Size

10 µm and 30 µm

40 to 60 µm

Temperature Metric Range

4 to 40°C

Operating Temperature

4Â°C-60Â°C

Operating Flow Rate, Recommended

1.3 mL/min

0.5 mL/min

Particle Size

60 Âµm-165 Âµm

45 Âµm-165 Âµm

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

PoreSize

40 to 60 µm

Ion exchanger

S (cation exchanger)

pH Stability Working Range

3â€“12

Pressure Limit

20 bar (2 MPa, 290 psi)

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Material

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

Signal Word

Warning

Storage Conditions

Store at 2Â°C to 8Â°C

4 - 30Â°C

Volume

2-6 ml

100-500 Âµl

5-20 ml

400 Âµl - 2 ml

Volume MetricBed

≈2.4mL

RIDADR

NONH for all modes of transport

Recommended Flow Rate

1 ml/min

Operating Flow Rate, Max

1.0 mL/min

2.6 mL/min

Theoretical Plates

> 13000 m-1

5000 m-1

Type

Gravity Chromatography Column

Chromatography Column

Liquid Chromatography (LC)

R-CODES

36/37/38

Volume Metric Bed

≈2.4mL

Wetted Part

yes

Total binding capacity

~50 mg hIgG/mL resin

P-CODES

P305 + P351 + P338

S-Codes

WGK

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

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