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Absorbance

0.02 AU max. at 330nm

Temperature

45°C

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Applications

HPLC: suitable

LPLC: suitable

solid phase extraction (SPE): suitable

gas chromatography (GC): suitable

Application

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Cell isolation

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Application_4

Chromatography system|Manual

Cell isolation

Bed Height

600 mm

100 mm

25 mm

Bed Volume

5 mL

320

120

1 mL

Assay

=99%

Base Style

Flat Bottom

Insert Vial

Bed Support

Filter

Net

Assay Percent Range

99.8% min by GC

Bed Dimensions

1.6 x 2.5 cm

16 x 25 mm

26 x 600 mm

16 x 100 mm

16 x 600 mm

7 x 25 mm

10 x 100 mm

26 mm x 400 mm

6.4 x 100 mm

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

At least 10 mg histidine-tagged protein/ml sedimented medium.

Average sample loading capacity

400 mg

50 mg

Autoignition Temp

800 °F

~800 °F

Bed Height With Two Adaptors

0 to 200 mm

58 mm - 106 mm

96 mm-156 mm

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

Up to 20 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

BioProcess resin

yes

Boiling Point

189°C

2230 C

Capacity

1.3 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

4.7 meq/g by dry weight

=1.1 meq/mL by wetted bed volume

200 µL

0.8 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

=25 % absorption capacity (water)

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

3.0 meq/g by dry weight

1.7 meq/mL by wetted bed volume

Bore Size (mm)

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Autoclavable

Not Autoclavable

CarbonLoad

11%

18%

20%

15%

Bed Height With One Adaptor

0 mm-29 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

Brand

Axygen®

Buffer

Running Buffers

CAS_NO

104219-63-8

112926-00-8

39389-20-3

1343-88-0

7631-86-9

69011-20-7

75-09-2

9052-95-3

9003-70-7

CAS Registry Number 1

7631-86-9

67-68-5

Certificate of Analysis

Yes

Chem AlertStorageSymbol

Gray

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chem Alert Storage Symbol

Gray

ChEBI

CHEBI:28262

Chemical Name or Material

Silica Gel

Dimethylsulfoxide

Chemical Stability

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Commonly used aqueous buffers

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Chemical Substance ID

24857358

24852837

329763462

24870506

329750331

329759187

329759181

329763461

Chromatography technique

Antibody affinity chromatography

GST-tagged protein purification

Ion Exchange Chromatography

Histidine-tagged protein purification

Hydrophobic Interaction Chromatography

Group specific affinity

Affinity coupling

Closure Size

13 mm

20 mm

8-425

11 mm

9 mm

Closure Color

Black

Silver

Blue

Color

Gold

Silver

White

Colorless to slightly yellow

Grey

Yellow

Colorless

N/A

Brass

Natural

Red

Amber

Black

Clear

Column i.d.

50 mm

10 mm

16 mm

26 mm

5 mm

Column Type

capillary non-polar

packed GC

capillary chiral

Reversed Phase

Mixed Mode

capillary polar

capillary extremely polar

Closure Type

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Crimp Cap

Screw Cap

Screw

ClosureMaterial

Polypropylene

Chemical/Physical Resistance

Temp. Limits:. -10 C to 180 C isothermal and programmed

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Composition

Discovery ® DSC-18, 400 mg

Tris Base, Sodium Chloride

bed wt., 500 mg

magnesium sulfate, 150 mg

Closure Material

Polypropylene

Plastic

Polyethylene

Aluminum

Application_5

Chromatography system|Manual

Column Capacity

< 1 mg/mL

Column Format

Analytical Column, EASY-Spray

Analytical Column

Column volume

1 mL

8 mL

Color

Gold/Brown

Decomposition Information

>190°C

ClosureSize

11 mm

9 mm

ClosureType

Screw

Snap Cap

Screw Cap

Screw Top

Density Max.

1.2 g/ml

Compatibility

Compatible with most QuEChERS shakers.

Diameter Metric

2.1 mm

75 µm

4.6 mm

Concentration

20 X

0.20 µm

0.12 µm

Diameter Metric Opening

9 mm

11 mm

EC Number

215-681-1

238-878-4

215-609-9

200-838-9

231-153-3

231-545-4

262-373-8

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Endcapped

Proprietary

Endcapped

Yes

Feature

closure type screw top vial

Endcapped

syringe type Sample Lock

septum type Thermogreen ® LB-2

closure type black phenolic solid top cap

without needle Luer tip

needle type N (cemented)

Not Endcapped

preconditioned

Diameter Metric Inner

12 mm

15 mm

6.6 mm

Density

1.100g/cmÂ³

Estimated Shelf Life from Manufacture Date

5 years

FilmThickness

1 µm

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

2 ml/min - 10 ml/min

1.6 mL/min

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

Diameter Metric Outer

12 mm

8 mm

15 mm

COLOR

Blue

Flow Velocity

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 150 cm/h

<1800 cm/h

15 cm/h|15 cm/h

< 450 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h

â‰¤ 600 cm/h

For Use With

ELSD, UV Detector

Format_6

Prepacked column

Kits

Bulk resin

Prepacked columns

Format

Liquid

matrix material

Exclusion Limit Peptides

4 x 10Â³

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Form

liquid

fine powder

powder

powder or crystals

fine crystals

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

Formula

O2Si

Service & Support

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

CH2Cl2

MgO₃Si

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

Diameter MetricOpening

9 mm

Endotoxin Activity Max.

0.12 EU/mg

Formula Weight

78.13

100.39

60.08

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Empirical Formula

MgO3Si

Flash Point

87°C (188.6°F)

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Height English

1.25 in.

1.69 in.

1.77 in.

1.57 in.

Hold-up Volume of Membrane

<5 Î¼l

<10 Î¼l

<20 Î¼l

Housing

Polypropylene (PP)

Grade

certified reference material

HPLC

high-purity grade (Davisil Grade 646)

Standard

high-purity grade (Davisil Grade 633)

technical grade

for column chromatography

high-purity grade

high-purity grade (Davisil Grade 62)

high-purity grade (Davisil Grade 923)

H-Codes

H317

H319

H371

H315-H319-H335

H372

H351

Height Metric

40 mm

Height Metric

45 mm

43 mm

32 mm

Health Hazard 2

WARNING!

Identification

Pass Test

Graduations

0.5, 1, 1.5 mL

Graduated

Group To be Coupled

-NHâ‚‚

Guidance

For AKTAprime

For one column with direct mounting to the flow cell.

Inlet id

2 mm (0.08 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Weak anion exchanger

Weak cation exchanger

Strong anion exchanger

Weak anion

Ionic Capacity

0.13-0.17 mmol/ml

0.18-0.24 mmol Cl^-/ml medium

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.18-0.25 mmol H⁺/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.09-0.13 mmol H⁺/ml medium

Length Metric

50 mm

100 mm

51 mm

250 mm

30 m

Ligand Coupling Method

Dextran surface extenders

Ether linkages and hydrophilic spacer arm

Ether linkages

Reductive Amination

Maleimide activation

Epoxy activation

Cyanogen bromide activation

Ligand Density

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

3 mg GammaBind G, type 3/ml medium

6 mg rProtein A/ml medium

~ 5 mg/mL resin

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Ligand_7

Protein A

Protein A, alkali stabilized

Protein L

Protein G

Protein A, alkali stablilized

Graduations

0.5 mL

Includes

3.5 in. Coil

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Instructions supplied with the concentractors

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Vials and Closures

Vial: C4015-1Closure: C4015-75A

Insert Type

250µL Fused Insert

Pre-Slit

350µL Fused Insert

Kit Contents

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

Liner Material

Aluminum/Silicone

PTFE/Silicone

None

PTFE/Rubber

PTFE/Silicone/PTFE

Polypropylene

Length Metric

150 mm

20 mm

Marking Spot

Yes

Material

aluminum liner

blunt tip Kel-F needle (point style 3)

clear glass bottle

polypropylene hardware

red PTFE/silicone septum

stainless steel hardware (column)

Tedlar ® PVDF film

white PTFE/silicone

white rubber liner

Clear Glass

Plastic/Stainless Steel

PTFE

silicone body

stainless steel TD tube (includes screens)

Acetal plastic (POM)

blue polypropylene cap

cone tip, side port stainless steel needle (point style 5)

N/A

Silicone

Brass

PEEK

Phenolic

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

polypropylene tube

polypropylene body

(Multi-Layer Foil: (1) 0.002" Polyethylene (2) 0.0003" Aluminum Foil (3) Polyethylene (4) Aluminum (metalized) (5) 60 Gauge Nylon)

black polypropylene cap

clear glass insert

glass TD tube

Polycarbonate (PC)

SilTite

stainless steel frit

clear glass vial

Mixed

PTFE/silicone septum

stainless steel column

Glass

glass body

PE frit (20 µm porosity)

bevel tip stainless steel needle (point style 2)

Fused silica

Silica Gel

Stainless Steel

stainless steel hardware

VG2, 60% Vespel/40% Graphite

Borosilicate glass

Polypropylene

Polypropylene (PP)

PTFE frit

Ligand

Carboxymethyl

Calmodulin

Strep-Tactin XT

Native Protein A

TALONÂ® Superflow Cobalt-IMAC

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Porcine heparin

Recombinant protein A (E.coli)

Sulphopropyl

Cibacron Blue

Quaternary amine

Diethylaminoethyl

Alkali-stabilized Protein A-derived (E. coli)

Glutathione and 10-carbon linker arm

Phenyl

Ligand Concentration

~25 Âµmol phenyl/mL resin

~4mg heparin/mL resin

~45 Âµmol phenyl/mL resin

Material Septa

PTFE/Silicone

PTFE/Silicone/PTFE

Matrix

Rigid, cross-linked agarose, spherical

6% Cross-Linked Agarose

Highly cross-linked agarose with dextran surface extender

Highly crossed-linked agarose, 6%

Max Pressure

17400 psi (1200 bar)

2000 psi

4500 psi (310 bar)

900 psi

5800 psi (400 bar)

11600 psi (800 bar)

Matrix

agarose, 6%, spherical

Composite of cross-linked agarose

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

2% agarose

Cross-linked dextran

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

cross-linked, 6% agarose particles

Highly cross-linked agarose

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked agarose, 4%, spherical

Max Temperature

330/350°C for Standard Columns; 430°C for Metal Columns

40°C

Max Temperature English

122°F

MDL Number

MFCD00132718

MFCD00132726

MFCD00132722

MFCD00145831

MFCD03096474

MFCD00132707

MFCD00132771

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00000881

MFCD00145841

MFCD00011232

MFCD00146442

MFCD00078249

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand

Recombinant GammaBind G type 3 lacking albumin-binding region

Linear Formula

MgO3Si

(C10H12 · C10H10 · C8H8 · C3H9N)X

(C10H12 · C10H10 · C8H8)x

(CH₃)₂SO

Material

100% Dimethyl Polysiloxane

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

IUPAC Name

methylsulfinylmethane

Inlet Connector

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

IOD-Codes

Max Temperature

445°C

MarkingSpot

Yes

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Molecular Weight

60.08

100.39

0.0

84.93

78.129

Length English

18 in.

Ligand Type

Monomeric C18

Operating Flow Rate, Max

< 4 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

Operating pH

0 - 9

1 - 8

2 - 8

2 - 7.5

3.5 - 7.0

3.8 - 6.8

1 - 9

2 - 9

0 - 14

Optical Activity

~7 (5% in H 2 O, suspension)

6.5 (5% in slurry)

7.0 (5% in slurry)

Order code

SH30031.01

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

For research use only.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Percoll PLUS is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Pack size

2 x 1 ml 10% slurry

50 mL

1 Ã— 320 mL

1 Ã— 4.7 mL

2 x 1 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1 piece

20 mL

300 mL

1000 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

500 mL

100 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

100 g

5 mL

5 Ã— 1 mL

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Fully Porous, Base-Deactivated Silica

Spherical, Nanopolymer Hybrid Silica

Partical Size Dry

18 Âµm-111 Âµm

>100 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

Particle Size

3 µm

60 Î¼m-200 Î¼m

2 µm

24 Âµm-44 Âµm

60 Âµm-165 Âµm

24-44 Âµm

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

60 Î¼m-160 Î¼m

Particle size, d_50V

~90 Âµm

34 Âµm

~75 Âµm

~34 Âµm

~34 Î¼m

~85 Âµm

~90 Î¼m

~ 9 Âµm

~ 8.6 Âµm

pH stability, operational

2â€“10

pH 6 to 10

3â€“10

3â€“12

3â€“9

2â€“9

4â€“13

4â€“9

2â€“12

2â€“13

4â€“12

3 to 12

3â€“13

Physical State

Liquid

solid

liquidSuspension

Pore Size

110 Å pore diameter

60 Å pore size

~0.55 mL/g pore volume

120 Å

90 Å

100 Å

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

100 Å

150 Å

90 Å mean pore size

0.75 cm 3 /g pore volume

180 Å

60 +?

60 Å mean pore size

70 Å pore size

0.7-0.85 cm 3 /g pore volume (approx.)

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

200 Å

160 Å

1000 Å

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Product Line

Environmental Express

Pierce

Easy Spray

ProductLine

Assembled Kit

TraceGOLD

Acclaim

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Quality

wet

monodispersed

with moisture indicator free of heavy metals

dry

Quantity

1 L

1000

1000/Cs.

200 pack

1000/Pk.

100/Pk.

500 mL

Quantity

200/Pk.

Each

100 g

144 Pack

100

200

100 Pack

Recommended Flow Rate

5 ml/min

1 ml/min

Refractive Index

1.4770 to 1.4800 (at 25°C)

Model

725

Sample Volume

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

<= 1.5 ml

<= 13 ml

4 to 50 ÂµL

<= 15 ml

<= 5 ml

< 0.5 mL

< 5 mL

600 Âµl

25 to 500 ÂµL

Septum

Aluminum/Silicone

PTFE/Silicone (0.75mil)

None

Operating Flow Rate, Recommended

0.75 mL/min

0.075 mL/min

0.80 mL/min

1.3 mL/min

0.5 mL/min

Sterilization

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

0.1 Âµm sterile filtered

Storage

+4Â°C to +8Â°C

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

20 to 25Â°C, Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative

20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2-8C

4Â°C to 8Â°C

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

Outlet id

1.55 mm (0.06 in)

1.0 mm (0.04 in)

0.75 mm (0.029 in)

Storage Spec

2°C to 8°C

p H

2 to 8

7.5 pH

2.5 to 7

Surface Area

~500 m 2 /g

300 m²/g

100 m 2 /g

500 m 2 /g

250-350 m 2 /g

530 m 2 /g

300 m 2 /g

470-530 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

100 m²/g

22 m 2 /g

320 m 2 /g

450 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

170 m 2 /g

289 m 2 /g

450 m 2 /g

480 m 2 /g

Packaging Layout

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

1 kg in poly bottle

1, 6x1, 2.5 kg in poly bottle

2.5, 5, 25 kg in fiber drum

100, 500 g in poly bottle

pH ligand fully charged

< 9

Entire pH range

Above 6

Below 9

pH stability, CIP

3â€“10

4â€“13

2â€“14Co²⁺-stripped medium

2â€“9

1â€“14

2â€“14

2â€“13

2â€“13.7

3â€“12

2â€“10

3â€“14

Porosity

10 or 80 Âµm

10 Î¼m

20 Î¼m

7 Î¼m

Resin Type

Sepharose High Performance

MabSelect

Capto

Sepharose Fast Flow

Intrinsic Viscosity

~ 17

No per Pack

100

Osmolality

< 25 mOsm/kg

Pictogram

GHS07

GHS08

Preslit

Yes

Pressure/Flow Specification

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Length English Needle

2 in.

Storage Conditions

At room temperature. Precipitation can occur at low temperature.

4â€“30Â°C

4ËšC to 30ËšC, 20% ethanol

4 - 30Â°C

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Vent

On inlet

Volume

1 mL

0.5 mL

2-6 ml

2 mL

25 mL

100-500 Âµl

5-20 ml

400 Âµl - 2 ml

22 mL

3 mL

50 mL

6 mL

Volume MetricTotal

2 mL

Volume_28

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

5-20 ml

Septum

Red PTFE/White Silicone

Melting Point

18.4°C

Needle Point Style

Blunt

>1600 C

Solubility Information

Solubility aq. soln.: insoluble.

Pack Size

Packaging

Amber glass

Percent Purity

≥99.9%

7.0 to 7.4

pH Range

5.5â€“7.5

8.5â€“9.5

8.9â€“9.9

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

System

Sepax C-Pro|Sefia|Sepax 2

Noper Pack

100

ParticleSize

3 µm

5 µm

pH Stability Cleaning-in-Place (CIP)

1 to 14

PoreSize

300 Å

No per Pack

100

Particle Shape

Spherical

Particle Size in Methanol Min

Theoretical Plates

> 43 000 m^-1

> 48 000 m^-1

> 13000 m-1

5000 m-1

> 38 000 m^-1

Osmolality

288-310 mOsm/kg

< 30 mOsm/kg

P-CODES

P280

P314

P305 + P351 + P338

Pressure Limit

2 MPa

20 bar (2 MPa, 290 psi)

Particle Size in Methanol Max

163

Phase

Reversed Phase

Reversed Phase and Ion Exchange

Sealing

Heat-fused

Purity

>=99%

Molecular Formula

C2H6OS

O2Si

R-CODES

36/37/38

48/20

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Stationary Phase

C18

Reversed Phase/Anion Exchange/Cation Exchange

S-Codes

26-36

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

SMILES

CS(=O)C

Residueafter Evaporation

5ppm max.

Signal Word

Warning

Danger

Shelf Life

24 Months

Thread Size

8-425

10-425

20 mm

9 mm

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Volume MetricResidual

<170µL

WGK

nwg

Recommended maximum operational flow velocity

300 cm/h

Pub Chem CID

679

Swelling factor

~1.7

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Purity GradeNotes

Mobile phase for HPLC applications

Stokes Radius

~ 10

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

Synonyms

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Temperature

60°C

Synonym

DMSO

Preslit

Type

Analytical Column

HPLC Column

Liquid Chromatography (LC)

Support System

Polypropylene (PP)

USP Type

Viscosity

1.98 mPa/s at 25Â°C

PurityGrade Notes

Mobile phase for HPLC applications

Volume Metric

4 mL

2 mL

Volume Metric Total

2 mL

Volume Metric Residual

<170µL

Volume MetricUsable

1.5 mL

4 mL

Vapor Density

<1 (vs air)

Viscosity Max

Volume Metric Usable

1.5 mL

Wetted Part

yes

Volume Metric

2.0 mL

Water

0.04% max.

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Volume Metric Working

1.8 mL

Total Time of Preparation

< 30 min/6 preps

U SPType

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Volume English

1 dr.

Total binding capacity

~50 mg hIgG/mL resin

VialType

11 mm Crimp Top Vial

9 mm Target DP Vial

9 mm SureStop Vial (AVCS)

Operating Pressure Max

4.1 bar (60 psi)

RTECS Number

VV7315000

Titratable Acid

0.001mEq/g max.

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