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Search results for : PCR Supplies

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Volume

200µL

Capacity

0.2 mL

Auto Restart On Power Failure

Yes

Cap Material

Polypropylene

Block Uniformity

Block at 55°C: ±0.3°C

Bottom Style

Conical

Block Description

Four sets of 96 x 0.2 mL tubes or four 96-well plates

Binding Capacity

12 µg Based on specified 15 µL aliquot of supplied magnetic bead suspension = 1.5 mg beads @ 100 mg/mL

Brand

Axygen®

Life Sciences

Certificate of Analysis

Yes

CAS_NO

163795-75-3

Content AndStorage

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient f

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

S

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.

G?° 4 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
G?° 1 x 0.5 mL DMSO
G?° 4 x 1.25 mL Water, nuclease free

Store at -20°C.

• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
• 10 mM dNTP Mix

Store at -5 to -30°C.

The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 ++M Forward GAPDH Primer, 10 ++M Reverse GAPDH

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20-°C.

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRul

Contains:
• 4 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 4 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.

Depth Cm

Depth In

Concentration

100 mM

10 mM

Content And Storage

Contains:
• 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 2 x 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 10 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (suffic

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5

Color

White

Clear

Concentration

FidelityvsTaq

25 X

2 X

52X

52 X

GCRich PCRPerformance

High

Medium

DetectionMethod

SYBR

GCRichPCRPerformance

Low

High

Medium

For Use With Application

For PCR application

PCR product analysis

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

Functional Testing

Each batch of PuReTaq Ready-To-Go PCR Beads is tested in a real-time PCR assay to ensure its ability to generate a specific PCR product with Î» DNA; and with negative control samples (untemplated reactions) to ensure the lowest possible levels of either e

Fidelityvs Taq

1 X

EC Number

231-791-2

Format

0.2mL Tube with hinged cap

Hazardous

Yes

Hot Start

Built-In Hot Start

Height Cm

53.5

Heated Lid Pressure

Adjustable

Height In

21.0625

For Use With Equipment

7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus System, StepOne System

Heated Lid Temperature

35 to 115°C, or off

Includes

2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO

Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25µL final volume, the concentration of each dNTP

2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO

2 x 1250µL GoTaq Green MasterMix, 2X, 2 x 1250µL Nuclease free Water, blue dye and a yellow dye, GoTaq DNA Polymerase, dNTPs, MgCl2 and reaction Buffers in a single tube

Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution

Kit Contents

Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10

Bulk first-strand cDNA reaction mixes (5)DTT solutionpd(N)₆ primerNot I-d(T)18 bifunctional primermRNA standardRNase-free water

Ready-To-Go GenomiPhi V3 cakes, 1 Ã— 96-well plate; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Includes LabelorDye

Yes

Labelor Dye

Unconjugated

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Number of Tests

55 reactions

Pack size

55 reactions

96 reactions

Includes Labelor Dye

Yes

Memory

1000 programs max

Product Line

DreamTaq

Phusion

RevertAid

Phire

Maxima

MDL Number

MFCD00284715

Molecular Weight

0.0

Quantity

100 rxn

100U (2U/-µL)

200 rxns

100 Units

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

500U (2U/µL)

1 Kit

1mL (Total: 40-µmol)

100 x 50µL rxns

1250 x 50++L rxns

100 reactions

4 x 1mL (4 x 100 -µmol of 100mM solution)

200 x 50µL rxns

ReactionFormat

Standalone

Separate Components

SuperMix or Master Mix

ReactionSpeed

Fast

Standard

Max Heating Rate

3.4°C per second

Sample Type

DNA, dsDNA

RNA

Size

1,000 reactions

100u

20ml

1 system

10,000u

250 preps

200microl

25ml

500u

40 reactions

40µmol each

100 reactions

50 preps

5ml

1,000 preps

20microg

50 preps/25 extractors

500 reactions

10µmol each

12.5ml

1,000microl

200micromol each

5,000u

20 reactions

2,500u

250 preps/100 extractors

No ofReactions

200 reactions

100

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Label Type

Unlabeled

Output

USB port

Polymerase

Phire Hot Start II DNA polymerase

Phusion U Hot Start DNA Polymerase

Phusion Hot Start II High-Fidelity DNA Polymerase

DreamTaq DNA Polymerase

Taq DNA Polymerase

Phusion Blood II DNA Polymerase

Phusion Hot Start II DNA Polymerase

Overhang

Blunt

3'-A

Power Hz

50/60

Power Vac

100 to 240

Physical State

Solid

Product Family

Axygen® PCR Strip Tubes

Axygen® PCR Tubes and Caps

Product Size Avg

> 10 kb

Product Type Specs

KOD Hot Start Master Mix

Qty/CS

1250 / Cs

10000 / Cs

Qty/Pk

125 / Pk

1000 / Pk

Min Gradient C

Quantity

250 x 50Î¼L rxns

100 x 20Î¼L rxns

PCRMethod

qPCR

Size

1.5mL

200 reactions

Number Of Blocks

Select Product

Multiwell Plate, 5 x 96 Reactions

0.2 ml hinged tube with cap, 96 Reactions

Shipping Condition

Approved for shipment on Wet or Dry Ice

Dry Ice

SufficientFor

20 reactions

100 reactions

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

Storage Requirements

Ambient temperature in pouch with desiccant

-20°C

-30 to -10°C

Width in

27.5629

Storage Temperature

Room Temperature

Reaction Time

1.5-2 h

Reaction Format

SuperMix or Master Mix

Refrigerated/Frozen

Frozen

Reverse Transcriptase

RevertAid

Sterile

Storage Conditions

-15 to -30Â°C

Ambient temperature in pouch with desiccant

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

Tests Per Kit

Width cm

Min Temperature C

No of Reactions

Max Gradient C

Max Temperature C

100

Optimal ReactionTemperature

42° C

Reaction Speed

Fast

Temperature Accuracy

50°C: ±0.25°C

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

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Contact the GSS Team Today!
GSS-Sales@govsci.com | 800.248.8030 | www.govsci.com

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