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Search results for : PCR Supplies

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Volume

200µL

Auto Restart On Power Failure

Yes

Block Uniformity

Block at 55°C: ±0.3°C

Binding Capacity

12 µg Based on specified 15 µL aliquot of supplied magnetic bead suspension = 1.5 mg beads @ 100 mg/mL

Capacity

0.2 mL

Bottom Style

Conical

CAS_NO

163795-75-3

Block Description

Four sets of 96 x 0.2 mL tubes or four 96-well plates

Brand

Life Sciences

Axygen®

Cap Material

Polypropylene

Content AndStorage

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 ++M Forward GAPDH Primer, 10 ++M Reverse GAPDH

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20-°C.

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient f

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRul

Contains:
• 4 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 4 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

S

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.

G?° 4 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
G?° 1 x 0.5 mL DMSO
G?° 4 x 1.25 mL Water, nuclease free

Store at -20°C.

• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
• 10 mM dNTP Mix

Store at -5 to -30°C.

Certificate of Analysis

Yes

Concentration

100 mM

10 mM

Content And Storage

Contains:
• 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 2 x 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 10 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (suffic

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5

Color

White

Color

Clear

DetectionMethod

SYBR

Depth Cm

Depth In

EC Number

231-791-2

FidelityvsTaq

52X

2 X

52 X

25 X

For Use With Application

For PCR application

PCR product analysis

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

For Use With Equipment

7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus System, StepOne System

Format

0.2mL Tube with hinged cap

GCRichPCRPerformance

Medium

High

Low

Fidelityvs Taq

1 X

Functional Testing

Each batch of PuReTaq Ready-To-Go PCR Beads is tested in a real-time PCR assay to ensure its ability to generate a specific PCR product with Î» DNA; and with negative control samples (untemplated reactions) to ensure the lowest possible levels of either e

GCRich PCRPerformance

Medium

High

Heated Lid Pressure

Adjustable

Hazardous

Yes

Height Cm

53.5

Hot Start

Built-In Hot Start

Height In

21.0625

Heated Lid Temperature

35 to 115°C, or off

Includes LabelorDye

Yes

Kit Contents

Bulk first-strand cDNA reaction mixes (5)DTT solutionpd(N)₆ primerNot I-d(T)18 bifunctional primermRNA standardRNase-free water

Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10

Ready-To-Go GenomiPhi V3 cakes, 1 Ã— 96-well plate; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Labelor Dye

Unconjugated

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Includes

2 x 1250µL GoTaq Green MasterMix, 2X, 2 x 1250µL Nuclease free Water, blue dye and a yellow dye, GoTaq DNA Polymerase, dNTPs, MgCl2 and reaction Buffers in a single tube

2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO

Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution

2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO

Room temperature stable beads containing ∼ 2.5 units of recombinant PuReTaq DNA Polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction Buffer, When a bead is reconstituted to a 25µL final volume, the concentration of each dNTP

PCRMethod

qPCR

Product Line

RevertAid

Maxima

DreamTaq

Phusion

Phire

Quantity

1mL (Total: 40-µmol)

4 x 1mL (4 x 100 -µmol of 100mM solution)

100 Units

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

500U (2U/µL)

200 x 50µL rxns

100 x 50µL rxns

100 Reactions

100U (2U/-µL)

1250 x 50++L rxns

100 rxn

200 rxns

1 Kit

Quantity

250 x 50Î¼L rxns

100 x 20Î¼L rxns

ReactionFormat

SuperMix or Master Mix

Separate Components

Standalone

ReactionSpeed

Standard

Fast

Select Product

0.2 ml hinged tube with cap, 96 Reactions

Multiwell Plate, 5 x 96 Reactions

Size

40 reactions

40µmol each

20 reactions

2,500u

250 preps/100 extractors

1,000 preps

20microg

50 preps/25 extractors

500 reactions

1 system

1.5ml

10,000u

250 preps

10µmol each

12.5ml

1,000 reactions

100u

20ml

200microl

25ml

500u

1,000microl

200micromol each

5,000u

100 reactions

200 reactions

50 preps

5ml

Min Temperature C

Includes Labelor Dye

Yes

Label Type

Unlabeled

No of Reactions

Polymerase

Phusion Hot Start II DNA Polymerase

Phusion Hot Start II High-Fidelity DNA Polymerase

Phusion Blood II DNA Polymerase

Phire Hot Start II DNA polymerase

Phusion U Hot Start DNA Polymerase

DreamTaq DNA Polymerase

Taq DNA Polymerase

Memory

1000 programs max

Max Heating Rate

3.4°C per second

MDL Number

MFCD00284715

Max Gradient C

Max Temperature C

100

Overhang

3'-A

Blunt

Molecular Weight

0.0

Min Gradient C

Number Of Blocks

Storage Requirements

-30 to -10°C

Ambient temperature in pouch with desiccant

-20°C

Shipping Condition

Approved for shipment on Wet or Dry Ice

Dry Ice

No ofReactions

200 reactions

100

Optimal ReactionTemperature

42° C

Pack size

96 reactions

55 reactions

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Tests Per Kit

Reaction Speed

Fast

Sample Type

RNA

DNA, dsDNA

Power Hz

50/60

Power Vac

100 to 240

Temperature Accuracy

50°C: ±0.25°C

Number of Tests

55 reactions

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

Output

USB port

Physical State

solid

Product Family

Axygen® PCR Strip Tubes

Axygen® PCR Tubes and Caps

Product Size Avg

> 10 kb

Reaction Format

SuperMix or Master Mix

Refrigerated/Frozen

Frozen

Product Type Specs

KOD Hot Start Master Mix

Qty/CS

1250 / Cs

10000 / Cs

Storage Temperature

Room Temperature

Qty/Pk

1000 / Pk

125 / Pk

Reaction Time

1.5-2 h

Reverse Transcriptase

RevertAid

Sterile

SufficientFor

20 reactions

100 reactions

Storage Conditions

-15 to -30Â°C

Ambient temperature in pouch with desiccant

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

Width cm

Width in

27.5629

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

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Contact the GSS Team Today!
GSS-Sales@govsci.com | 800.248.8030 | www.govsci.com

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