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Absorbance

0.02 AU max. at 330nm

Temperature

45°C

Application

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Highly suitable for preparative purification of histidinetagged recombinant proteins

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Bed Volume

120

1 mL

5 mL

320

Application_5

Chromatography system|Manual

Bed Height

25 mm

600 mm

100 mm

30 mm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application_4

Chromatography system|Manual

Cell isolation

Applications

LPLC: suitable

solid phase extraction (SPE): suitable

HPLC: suitable

ion chromatography: suitable

gas chromatography (GC): suitable

Capacity

1.7 meq/mL by wetted bed volume

=25 % absorption capacity (water)

1.3 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

200 µL

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

4.7 meq/g by dry weight

CarbonLoad

15%

11%

Base Style

Flat Bottom

Bed Support

Filter

Net

CAS Registry Number 1

67-68-5

7631-86-9

Assay

=99%

99.8%

Bed Dimensions

1.6 x 2.5 cm

7 x 25 mm

16 x 25 mm

26 x 600 mm

16 x 100 mm

16 x 600 mm

BioProcess resin

yes

Boiling Point

189°C

2230 C

Autoignition Temp

800 °F

Average sample loading capacity

50 mg

400 mg

Binding Capacity/ml Chromatography Medium

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

~ 13 mg ovalbumin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 10 mg histidine-tagged protein

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Bed Height With One Adaptor

225 mm-350 mm

525 mm-650 mm

85 mm-109 mm

CAS_NO

9003-70-7

9041-35-4

9050-97-9

39389-20-3

7631-86-9

112926-00-8

1343-88-0

9052-95-3

69011-20-7

104219-63-8

Assay Percent Range

99.8% min by GC

Bed Height With Two Adaptors

80 mm-350 mm

0 to 200 mm

Chemical Stability

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Commonly used aqueous buffers

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chem Alert Storage Symbol

Gray

Autoclavable

Not Autoclavable

Chem AlertStorageSymbol

Gray

Chemical Name or Material

Silica Gel

Dimethylsulfoxide

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Bore Size (mm)

Chromatography technique

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography

Histidine-tagged protein purification

Affinity coupling

Group specific affinity

Antibody affinity chromatography

GST-tagged protein purification

Closure Type

Crimp Cap

Screw Cap

Screw

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Color

Gold/Brown

Natural

Closure Size

9 mm

11 mm

13 mm

20 mm

COLOR

Blue

Column i.d.

5 mm

26 mm

10 mm

16 mm

50 mm

Closure Color

Black

Silver

ClosureMaterial

Polypropylene

Column Type

capillary chiral

Reversed Phase

capillary extremely polar

capillary polar

Mixed Mode

capillary non-polar

packed GC

Composition

Discovery ® DSC-18, 400 mg

bed wt., 500 mg

magnesium sulfate, 150 mg

Tris Base, Sodium Chloride

Diameter Metric Opening

11 mm

Diameter Metric Outer

12 mm

15 mm

8 mm

Closure Material

Polypropylene

Aluminum

Polyethylene

Plastic

Color

White

Amber

Black

Clear

Red

Colorless

N/A

Brass

Colorless to slightly yellow

Grey

Yellow

Gold

Silver

Column Capacity

< 1 mg/mL

Column Format

Analytical Column, EASY-Spray

Analytical Column

Column volume

1 mL

8 mL

Chemical Substance ID

24852837

24870506

329763461

329759181

329750331

329759187

24857358

329763462

Endotoxin Activity Max.

0.12 EU/mg

ClosureType

Screw Cap

Screw Top

Screw

Snap Cap

Diameter Metric Inner

15 mm

6.6 mm

12 mm

Flow Velocity

< 450 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

â‰¤ 600 cm/h

< 300 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

<1800 cm/h

15 cm/h|15 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 150 cm/h

<15 cm/h

Up to 40 cm/h

Density

1.100g/cmÂ³

Feature

needle type N (cemented)

Endcapped

syringe type Sample Lock

closure type screw top vial

preconditioned

Not Endcapped

septum type Thermogreen ® LB-2

3.6 mm orbit

KEL-F hub

closure type black phenolic solid top cap

septum replaceable Thermogreen LB-2

temp. limit 135 °C

without needle Luer Tip

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

Flow Rate

2 ml/min - 10 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

< 20 ml/min|< 20 ml/min

1 mL/min - 4 mL/min

1.6 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 20 ml/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

0.25 to 0.75 mL/min

0.8-3.9 ml/min

Form

fine powder

fine crystals

Powder

powder or crystals

Liquid

Compatibility

Compatible with most QuEChERS shakers.

Concentration

20 X

Formula

O2Si

Service & Support

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

MgO₃Si

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Diameter MetricOpening

9 mm

ClosureSize

11 mm

9 mm

0.12 µm

0.20 µm

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Temp. Limits:. -10 C to 180 C isothermal and programmed

EC Number

200-838-9

231-153-3

215-681-1

231-545-4

238-878-4

262-373-8

215-609-9

Diameter Metric

2.1 mm

4.6 mm

75 µm

Density Max.

1.2 g/ml

Estimated Shelf Life from Manufacture Date

5 years

FilmThickness

1 µm

Empirical Formula

MgO3Si

Flash Point

87°C (188.6°F)

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

Format

Liquid

Format_6

Kits

Prepacked column

Prepacked columns

Bulk resin

Endcapped

Proprietary

Yes

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Format

matrix material

Formula Weight

100.39

60.08

78.13

Decomposition Information

>190°C

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

H-Codes

H371

H317

H319

H351

H372

H315-H319-H335

Health Hazard 2

WARNING!

For Use With

ELSD, UV Detector

Grade

HPLC

certified reference material

Standard

high-purity grade (Davisil Grade 633)

Grade

High-purity Grade (Davisil Grade 923)

High-purity Grade

Technical Grade

High-purity Grade (7754)

Height Metric

45 mm

32 mm

43 mm

Hold-up Volume of Membrane

<10 Î¼l

<20 Î¼l

<5 Î¼l

Exclusion Limit Peptides

4 x 10Â³

Graduations

0.5, 1, 1.5 mL

Inlet Connector

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Stepped barb for 6 to 10 mm (1/4 to 3/8") tubing

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Insert Type

350µL Fused Insert

Fused Insert

250µL Fused Insert

Pre-Slit

IOD-Codes

For Use With Equipment

Environmental Express StepSaver

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Sulphopropyl

Porcine heparin

Cibacron Blue

Human IgG

Quaternary amine

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Diethylaminoethyl

Native Protein A

TALONÂ® Superflow Cobalt-IMAC

Alkali-stabilized Protein A-derived (E. coli)

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Carboxymethyl

Ligand Density

~ 5 mg/mL resin

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

6 mg rProtein A/ml medium

3 mg Protein A/ml medium

Approx. 13 Î¼mol/ml

3 mg GammaBind G, type 3/ml medium

2 mg Protein G/ml medium

MarkingSpot

Yes

Material

polypropylene body

clear glass vial

Glutathione Sepharose 4 B

Mixed

PTFE body

PTFE/silicone septum

(Multi-Layer Foil: (1) 0.002" Polyethylene (2) 0.0003" Aluminum Foil (3) Polyethylene (4) Aluminum (metalized) (5) 60 Gauge Nylon)

black polypropylene cap

clear glass insert

glass TD tube

Polycarbonate (PC)

SilTite

high-density polyethylene vial

PTFE/Silicone

Borosilicate Glass

Polypropylene

Polypropylene (PP)

PTFE/red rubber septum

stainless steel TD tube

Acetal plastic (POM)

blue polypropylene cap

cone tip, side port stainless steel needle (point style 5)

N/A

Amber Glass (Type 51A)

glass

glass body

PE frit (20 µm porosity)

PE frit (20/40 µm)

PTFE Luer lock

StableFlex fiber

Brass

PEEK

Phenolic

bevel tip stainless steel needle (point style 2)

Fused silica

Silica Gel

Stainless steel

stainless steel hardware

VG2, 60% Vespel/40% Graphite

Plastic/Stainless Steel

PTFE

Tedlar ® PVDF film

polypropylene hardware

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

cross-linked, 6% agarose particles

cross-linked agarose, 4%, spherical

2% agarose

Cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Rigid, cross-linked agarose, spherical

Matrix

Highly crossed-linked agarose, 6%

Highly cross-linked 4% agarose

Highly Cross-Linked Agarose

4% Agarose

4% Cross-Linked Agarose

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

MDL Number

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00132712

MFCD00011232

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00149897

MFCD00211881

MFCD00145586

MFCD03791384

MFCD00145841

MFCD00146442

MFCD00078249

MFCD00132707

MFCD00132722

MFCD00132726

MFCD00145831

Includes

Connectors for high- and low-pressure chromatography equipment and instructions.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

3.5 in. Coil

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Ionic Capacity

0.29-0.35 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Includes

100 vials and 100 seals with septa

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

Vials and Closures

Intrinsic Viscosity

~ 17

Ion Exchanger Type

Strong anion exchanger

Weak anion

Weak cation exchanger

Weak and strong anion

Weak anion exchanger

Strong cation exchanger

Length English Needle

2 in.

Length Metric

250 mm

400 mm

100 mm

60 m

750 mm

50 mm

Ligand Concentration

4.5-8 mg gelatin/ml drained resin

~4mg heparin/mL resin

~45 Âµmol phenyl/mL resin

Ligand Coupling Method

Epoxy

Maleimide activation

Epoxy activation

Cyanogen bromide activation

Triazine

Reductive Amination

Ether linkages

Ligand_7

2-mercaptopyridine

Protein A, alkali stablilized

Butyl, octyl, phenyl

Protein G

Protein L

Protein A

Protein A, alkali stabilized

Length English

18 in.

Length Metric

20 mm

500 mm

30 m

457 mm

51 mm

150 mm

Ligand

Recombinant Protein A (E.coli)

Ligand Type

Monomeric C18

Molecular Weight

60.08

0.0

100.39

60.08

78.129

Linear Formula

SiO2

(C10H12 · C10H10 · C8H8)x

(C10H12 · C10H10 · C8H8 · C3H9N)X

(CH₃)₂SO

MgO3Si

Material Septa

PTFE/Silicone/PTFE

PTFE/Silicone

Max Pressure

11600 psi (800 bar)

5800 psi (400 bar)

4000 psi (275 bar)

4500 psi (310 bar)

14500 psi (1000 bar)

17400 psi (1200 bar)

Liner Material

Polypropylene

PTFE/Rubber

PTFE/Silicone

Kit Contents

Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Aldolase (50 mg): 158 000 M_r
Ferritin (15 mg): 440 000 M_r
Thyroglobulin (50 mg): 669 000 M_r
Blue Dextran 2000 (50 mg

RNAspin Lysis Solution (Red sticker): 125 ml, RNAspin Wash Buffer I (Yellow sticker): 80 ml, RNAspin Wash Buffer II (Grey sticker): 3 Ã— 25 ml Add 100 ml Ethanol to each bottle; RNAspin Desalting Buffer (Blue sticker); 125 ml, RNAspin Desalting Reaction B

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

Length English

2 in.

Material

PE Frit

Stainless Steel Frit

Metal

Rubber

Stainless Steel Column

Stainless Steel Holder

Borosilicate Glass, PTFE, Polyethylene, FKM/FPM

PTFE Frit

Polypropylene Tube

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

Operating Flow Rate, Max

< 4 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

Operating pH

0 - 9

2 - 8

0 - 14

1 - 9

2 - 9

2 - 7.5

3.8 - 6.8

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

6.5 (5% in slurry)

Order code

SH30031.01

SH30031.02

SH30268.01

SH30588.01

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

HiLoad columns are prepacked XK columns

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Percoll PLUS is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

For research use only.

Pack size

2 x 1 ml 10% slurry

50 mL

10 mL

200 mL

25 mL

4 x 50 mL

4 x 53 mL

500 g

6 x 500 mL

1 Ã— 4.7 mL

2 x 1 mL

500 mL

100 g

5 mL

1 piece

20 mL

300 mL

100 mL

1000 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

5 Ã— 1 mL

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Solid Core, Ultrapure Silica

Type B Silica

Spherical, Nanopolymer Hybrid Silica

Partical Size Dry

18 Âµm-111 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

Particle Size

3 µm

2 µm

24-44 Âµm

24 Âµm-44 Âµm

60 Âµm-165 Âµm

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

60 Î¼m-160 Î¼m

Particle size, d_50V

~90 Âµm

34 Âµm

~85 Âµm

~75 Âµm

~90 Î¼m

~ 9 Âµm

~34 Âµm

~34 Î¼m

~ 8.6 Âµm

pH stability, operational

2â€“10

pH 6 to 10

2â€“13

4â€“12

3â€“12

3â€“9

2â€“12

2â€“9

4â€“13

4â€“9

3â€“10

3 to 12

3â€“13

IUPAC Name

methylsulfinylmethane

Physical State

Liquid

Solid

liquidSuspension

Material Coating

Polypropylene

Material Plunger

Stainless Steel

Max Temperature Metric

50°C

Pore Size

110 Å pore diameter

60 Å pore size

60 +?

60 Å mean pore size

70 Å pore size

~0.55 mL/g pore volume

120 Å

90 Å

0.7-0.85 cm 3 /g pore volume (approx.)

160 Å

100 Å

150 Å

90 Å mean pore size

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

0.75 cm 3 /g pore volume

180 Å

100 Å

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

1000 Å

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Product Line

Environmental Express

Pierce

Easy Spray

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Melting Point

18.4°C

ProductLine

TraceGOLD

Acclaim

Model

725

Quality

wet

monodispersed

with moisture indicator free of heavy metals

dry

Quantity

1 L

1000

1000/Cs.

Each

100

100 Pack

100/Pk.

1000/Pk.

Quantity

200/Pk.

200

144 Pack

100 g

500 mL

Recommended Flow Rate

5 ml/min

1 ml/min

Refractive Index

1.4770 to 1.4800 (at 25°C)

Sample Volume

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

600 Âµl

<= 1.5 ml

<= 15 ml

<= 5 ml

< 0.5 mL

< 5 mL

<= 13 ml

4 to 50 ÂµL

25 to 500 ÂµL

Septum

Aluminum/Silicone

None

No per Pack

100

No per Pack

1000

100

p H

2.5 to 7.5

8.5 pH

2 to 8

2.5 to 7

4 to 13

7.5 pH

Packaging Layout

100, 500 g in poly bottle

5, 25 kg in fiber drum

1 ft3 in fiber drum

25, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

1 kg in poly bottle

1, 6x1, 2.5 kg in poly bottle

Min Temperature English

50°F

Particle Shape

Spherical

Particle size distribution, wet

90â€“280 Âµm

Particle Size in Methanol Min

ParticleSize

2.2 µm

5 µm

3 µm

pH Stability Working Range

3â€“12

pH stability, CIP

1â€“14

2â€“14

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

2â€“10

3â€“14

2â€“14Co²⁺-stripped medium

2â€“9

Outlet id

1.55 mm (0.06 in)

1.0 mm (0.04 in)

0.75 mm (0.029 in)

Porosity

20 Î¼m

10 or 80 Âµm

7 Î¼m

10 Î¼m

Pressure/Flow Specification

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

pH ligand fully charged

Entire pH range

Above 6

Below 9

Operating Flow Rate, Recommended

0.5 mL/min

1.3 mL/min

0.075 mL/min

0.80 mL/min

Sterilization

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

0.1 Âµm sterile filtered

Storage

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

2-8C

4Â°C to 8Â°C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol

Osmolality

288-310 mOsm/kg

< 30 mOsm/kg

< 25 mOsm/kg

P-CODES

P280

P314

P305 + P351 + P338

Surface Area

~500 m 2 /g

300 m²/g

170 m 2 /g

300 m 2 /g

470-530 m 2 /g

100 m²/g

22 m 2 /g

320 m 2 /g

450 m 2 /g

100 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

250-350 m 2 /g

530 m 2 /g

289 m 2 /g

450 m 2 /g

480 m 2 /g

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Particle Size in Methanol Max

163

Phase

Reversed Phase

Reversed Phase and Ion Exchange

>1600 C

Pressure Limit

20 bar (2 MPa, 290 psi)

Preslit

Noper Pack

100

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

S-Codes

26-36

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Resin Type

Sepharose High Performance

Capto

Sepharose Fast Flow

MabSelect

Signal Word

Warning

Danger

Molecular Formula

C2H6OS

O2Si

PurityGrade Notes

Mobile phase for HPLC applications

pH Range

8.5â€“9.5

8.9â€“9.9

5.5â€“7.5

Residueafter Evaporation

5ppm max.

R-CODES

36/37/38

48/20

Shelf Life

24 months

Scale

Lab

Lab|Process development|Process

Lab|Process development

Lab|Process

Swelling factor

~1.7

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Storage Conditions

At room temperature. Precipitation can occur at low temperature.

4 - 30Â°C

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

pH Stability Cleaning-in-Place (CIP)

1 to 14

Pictogram

GHS08

GHS07

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Recommended maximum operational flow velocity

300 cm/h

PoreSize

300 Å

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Stationary Phase

Reversed Phase/Anion Exchange/Cation Exchange

C18

Synonyms

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Stokes Radius

~ 10

System

Sepax C-Pro|Sefia|Sepax 2

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

Vent

On inlet

Volume

1 mL

5-20 ml

400 Âµl - 2 ml

2 mL

25 mL

0.5 mL

2-6 ml

100-500 Âµl

22 mL

3 mL

50 mL

6 mL

Volume MetricTotal

2 mL

Volume_28

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

5-20 ml

Height English

1.77 in.

Identification

Pass Test

Preslit

Yes

Theoretical Plates

> 43 000 m^-1

5000 m-1

> 38 000 m^-1

> 48 000 m^-1

> 13000 m-1

Synonym

DMSO

Purity

>=99%

Volume Metric

2 mL

Volume Metric

2.0 mL

4 mL

Water

0.04% max.

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Height English

1.25 in.

Housing

Polypropylene (PP)

Septum

Red PTFE/White Silicone

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

SMILES

CS(=O)C

Solubility Information

Solubility aq. soln.: insoluble.

Thread Size

10-425

20 mm

9 mm

Volume Metric Working

1.8 mL

Total binding capacity

~50 mg hIgG/mL resin

VialType

11 mm Crimp Top Vial

9 mm Target DP Vial

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Volume Metric Residual

<170µL

Volume MetricUsable

1.5 mL

4 mL

Support System

Polypropylene (PP)

Total Time of Preparation

< 30 min/6 preps

Temperature

60°C

Type

Liquid Chromatography (LC)

HPLC Column

Analytical Column

U SPType

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Volume MetricResidual

<170µL

WGK

nwg

Wetted Part

yes

Vapor Density

<1 (vs air)

Viscosity Max

Volume Metric Usable

1.5 mL

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Volume English

1 dr.

Needle Point Style

Blunt

Pack Size

Packaging

Amber Glass

Percent Purity

≥99.9%

7.0 to 7.4

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

Sealing

Heat-fused

USP Type

Viscosity

1.98 mPa/s at 25Â°C

Volume Metric Total

2 mL

Operating Pressure Max

4.1 bar (60 psi)

RTECS Number

VV7315000

Titratable Acid

0.001mEq/g max.

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