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Chromatography

Chromatography Consumables

Reagents and Buffers

Buffers for Protein Methods

Chromatography Media

Specialized HPLC Reagents

Gas Chromatography Derivatization Reagents

Concentrated Buffers

Silylation Grade Solvents

Silylation Reagents

$0~$10(0)
$10~$25(3)
$25~$50(186)
$50~$75(53)
$75~$100(17)
$100~$200(89)
$200~$300(58)
$300~$400(28)
$400~$500(30)
$500~$750(47)
$750~$1000(31)
$1000~$5000(106)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Application

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

Additives

Without: Calcium, magnesium, phenol red

Without: Phenol red

Without: Calcium, magnesium

Application_5

Chromatography system|Manual

Bed Height

25 mm

600 mm

100 mm

30 mm

Bed Volume

1 mL

5 mL

320

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application_4

Chromatography system|Manual

Chromatography system

Cell isolation

Capacity

1.7 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

4.7 meq/g by dry weight

Binding Capacity/ml Chromatography Medium

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 10 mg histidine-tagged protein

CAS Registry Number 1

67-68-5

CAS_NO

1344-28-1

69011-19-4

9003-70-7

9041-35-4

9050-97-9

15461-27-5

39389-20-3

63181-98-6

65546-95-4

75-09-2

112945-52-5

7631-86-9

112926-00-8

1343-88-0

39339-85-0

11128-95-3

126850-14-4

9004-54-0

69011-20-7

104219-63-8

60177-39-1

62610-50-8

65099-79-8

69011-15-0

Assay

=99%

99.8%

BioProcess resin

yes

Boiling Point

189°C

2230 C

Autoignition Temp

800 °F

~800 °F

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Bead type

Mag Sepharose Magnetic Beads

Applications

ion chromatography: suitable

Average sample loading capacity

50 mg

400 mg

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

At least 10 mg histidine-tagged protein/ml sedimented medium.

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Bed Dimensions

7 x 25 mm

16 x 25 mm

26 x 600 mm

10 x 100 mm

6.4 x 100 mm

16 x 30 mm

16 x 100 mm

Assay Percent Range

99.8% min by GC

Chemical Stability

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Stable in all commonly used aqueous buffers

Commonly used aqueous buffers

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Chemical Substance ID

24852304

24854187

329756032

24854191

329752001

329752189

24857354

24867159

24852120

24854190

24854194

24857352

24863799

24865203

24852837

24854823

329759188

329763461

329759181

329759183

329759193

329768201

329759187

329768868

24854645

24857358

329763462

ChEBI

CHEBI:28262

Certificate of Analysis

Yes

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chem Alert Storage Symbol

Gray

Chem AlertStorageSymbol

Gray

Buffer

Running Buffers

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Stable in commonly used aqueous buffers, 1 M NaOH

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Chromatography technique

Hydrophobic Interaction Chromatography

Ion Exchange Chromatography

Histidine-tagged protein purification

Affinity coupling

Antibody affinity chromatography.

Group specific affinity

Antibody affinity chromatography

GST-tagged protein purification

Color

Gold/Brown

Composition

nitrogen content, =1.0%

Tris Base, Sodium Chloride

Disodium Hydrogen Phosphate, Sodium Chloride

COLOR

Blue

Chemical Name or Material

Dimethylsulfoxide

Density Max.

1.078 g/ml

1.135 g/ml|

1.2 g/ml

Column i.d.

6.4

26 mm

16 mm

Color

White

Colorless

Colorless to slightly yellow

Column volume

1 mL

8 mL

EC Number

215-691-6

200-838-9

215-681-1

231-545-4

238-878-4

215-710-8

231-717-9

Endotoxin Activity Max.

0.12 EU/mg

< 0.12 EU/ml

2 EU/ml

Flow Rate

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 mL/min

> 4 mL/min

2 ml/min - 10 ml/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 5 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 20 ml/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

Flow Velocity

< 450 cm/h

< 450 cm/h|< 450 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 500 cm/h

30-150cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 150 cm/h

<15 cm/h

Density

1.100g/cmÂ³

Form

fine powder

beads

fine crystals

Powder

powder or crystals

Liquid

Concentration

20 X

10 X

Formula

O2Si

Service & Support

ZrOCl₂·xH₂O

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

MgO₃Si

SiO₂

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Al2O3

CH2Cl2

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

Concentration

1 N in NaOH (Regenerant concentration)

Content And Storage

Store at room temperature.

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Estimated Shelf Life from Manufacture Date

5 years

3 years

8 years

Empirical Formula

MgO3Si

O2Si

Feature

ferrule type packed column

Flashpoint (°C)

> 240 C

Flash Point

87°C (188.6°F)

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

For all Biacore SPR systems.

Format

Liquid

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

For Use With

GC/MS

Format

matrix material

Gel Type

Tris-Glycine Gel

Formula Weight

100.39

60.08

78.13

Format_6

Prepacked column

Prepacked columns

Bulk resin

Decomposition Information

>190°C

Gel Compatibility

Novex® Tris-Glycine Gels, Tris-Glycine Gels, Precise Tris-Glycine Gels

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

H-Codes

H371

H319

H373

H372

Hazard s

Flammable, Irritant

Health Hazard 2

WARNING!

Grade

High-purity Grade (Davisil Grade 643)

High-purity Grade (Davisil Grade 923)

High-purity Grade

High-purity Grade (Davisil Grade 62)

Technical Grade

High-purity Grade (7754)

High-purity Grade (Davisil Grade 646)

Grade

HPLC

For column chromatography

certified reference material

high-purity grade (Davisil Grade 636)

nuclear grade

reagent grade

Standard

high-purity grade (Davisil Grade 633)

Group To be Coupled

-NHâ‚‚

Primary amines

Exclusion Limit Peptides

4 x 10Â³

IOD-Codes

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Sulphopropyl

Porcine heparin

Cibacron Blue

Human IgG

Quaternary amine

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Diethylaminoethyl

Native Protein A

Alkali-stabilized Protein A-derived (E. coli)

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Carboxymethyl

Ligand Density

~ 5 mg/mL resin

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

3 mg Protein A/ml medium

Approx. 13 Î¼mol/ml

3 mg GammaBind G, type 3/ml medium

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

cross-linked, 6% agarose particles

cross-linked agarose, 4%, spherical

2% agarose

Cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

Cross-linked agarose, 4%

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

agarose, 6%, spherical

Rigid, cross-linked agarose, spherical

Matrix

Highly crossed-linked agarose, 6%

Highly cross-linked 4% agarose

Highly Cross-Linked Agarose

4% Agarose

4% Cross-Linked Agarose

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

MDL Number

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00132712

MFCD00011232

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00149897

MFCD00211881

MFCD00145586

MFCD03791384

MFCD00145841

MFCD00084428

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00132707

MFCD00132771

MFCD00132722

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

Includes

Connectors for high- and low-pressure chromatography equipment and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Ionic Capacity

0.29-0.35 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.18-0.25 mmol H⁺/ml medium

0.13-0.17 mmol/ml

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Intrinsic Viscosity

~ 17

Ion Exchanger Type

Strong anion exchanger

Weak anion

Weak cation exchanger

Weak and strong anion

Weak anion exchanger

Multimodal weak cation exchanger

Strong cation exchanger

Ligand Concentration

4.5-8 mg gelatin/ml drained resin

~4mg heparin/mL resin

~45 Âµmol phenyl/mL resin

~25 Âµmol phenyl/mL resin

Ligand Coupling Method

Epoxy

Maleimide activation

Epoxy activation

Cyanogen bromide activation

Triazine

Reductive Amination

Ether linkages

Ether linkages and hydrophilic spacer arm

Dextran surface extenders

Ligand

Recombinant Protein A (E.coli)

Molecular Weight

60.08

0.0

100.39

101.96

60.08

178.13 (anhydrous basis)

84.93

78.129

Linear Formula

SiO2

(C10H12 · C10H10 · C8H8)x

(C10H12 · C10H10 · C8H8 · C3H9N)X

(CH₃)₂SO

MgO3Si

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

Ligand_7

Butyl, octyl, phenyl

Protein G

Protein L

Protein A

Protein A, alkali stabilized

Operating Flow Rate, Max

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

6.5 (5% in slurry)

Order code

SH30031.01

SH30031.02

SH30268.01

SH30588.01

SH30237.01

SH30588.02

SH30031.FS

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Percoll PLUS is for research use only.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

For research use only.

Pack size

2 x 1 ml 10% slurry

50 mL

10 mL

200 mL

25 mL

4 x 50 mL

500 g

6 x 500 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

500 mL

100 g

5 mL

1 piece

20 mL

300 mL

1.7 mL

100 mL

125 mL

25 g

1000 mL

15 g

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

1.5 g

5 Ã— 1 mL

6 x 1 L

Partical Size Dry

18 Âµm-111 Âµm

>100 Âµm

>20 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

50 Âµm-150 Âµm

Particle size, d_50V

~90 Âµm

34 Âµm

~85 Âµm

~75 Âµm

~90 Î¼m

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~ 8.6 Âµm

~50 Âµm

pH stability, operational

2â€“10

pH 6 to 10

2â€“13

4â€“12

3â€“12

3â€“9

2â€“12

2â€“9

4â€“13

4â€“9

3â€“10

3 to 12

3â€“11

3â€“13

IUPAC Name

methylsulfinylmethane

Physical State

Liquid

Solid

liquidSuspension

Metal Ion Capacity

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Melting Point

18.4°C

Material

N/A

PEEK

Plastic/Stainless Steel

Quality

wet

regenerated

monodispersed

dry

Quantity

1 L

500 g

Recommended Flow Rate

5 ml/min

1 ml/min

Refractive Index

1.4770 to 1.4800 (at 25°C)

Sample Volume

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

<= 5 ml

100 to 300 Î¼l

400 to 1000 Î¼l

<= 13 ml

4 to 50 ÂµL

25 to 500 ÂµL

Kit Contents

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

p H

8.5 pH

7.5 pH

Packaging Layout

100, 500 g in poly bottle

5, 25 kg in fiber drum

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

500 g in poly drum

2.5 kg in poly drum

2.5, 5, 25 kg in fiber drum

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

Particle size distribution, wet

90â€“280 Âµm

15â€“88 Âµm

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

Particle Size in Methanol Min

pH stability, CIP

1â€“14

2â€“14

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

2â€“10

3â€“14

3â€“13

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

2â€“12

Operating Flow Rate, Recommended

0.75 mL/min

0.5 mL/min

0.30 mL/min

1.3 mL/min

0.50 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

Pore Size

60 Å mean pore size

~0.55 mL/g pore volume

0.7-0.85 cm 3 /g pore volume (approx.)

150 Å

90 Å mean pore size

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

0.75 cm 3 /g pore volume

~0.8 cm 3 /g pore volume

110 Å pore size

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

Pressure/Flow Specification

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

Particle Size

60 Î¼m-200 Î¼m

60 Âµm

24-44 Âµm

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

pH ligand fully charged

< 9

Entire pH range

Above 6

Below 9

Pressure/flow characteristics

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Product Line

Pierce, Tween

Pierce™

Pierce™, Tween™

Pierce

Storage

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2-8C

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 100% Isopropanol

2 to 8Â°C, 20% Ethanol

Osmolality

288-310 mOsm/kg

< 30 mOsm/kg

< 25 mOsm/kg

276-298 mOsm/kg

Storage Spec

2°C to 8°C

P-CODES

P260

P314

P305 + P351 + P338

Surface Area

~500 m 2 /g

~30 m 2 /g

550 m 2 /g

300 m 2 /g

470-530 m 2 /g

175-225 m 2 /g

380 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

250-350 m 2 /g

530 m 2 /g

120 m 2 /g

289 m 2 /g

480 m 2 /g

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Particle size distribution, dry

50 â€“ 150 Âµm

20 â€“ 80 Âµm

Particle Size in Methanol Max

163

Operating pH

0 - 14

0 - 7

Osmolality

322-344 mOsm/kg

>1600 C

Purity

99.8%

Quantity

50 g

10 x 1 mL ampules

100 g

500 mL

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

S-Codes

26-36

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Resin Type

Sepharose High Performance

Capto

Sepharose Fast Flow

MabSelect

Sepharose CL-4B

Signal Word

Warning

Danger

Molecular Formula

C2H6OS

PurityGrade Notes

Mobile phase for HPLC applications

Synonyms

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Silica gel 110 NH2

Activated magnesium silicate; Magnesium silicate

Silica gel

Amberlite(R) IR120 hydrogen form

pH Range

8.5â€“9.5

8.9â€“9.9

5.5â€“7.5

Residueafter Evaporation

5ppm max.

R-CODES

36/37/38

48/20

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Shelf Life

24 months

12 Months

Scale

Lab

Lab|Process development|Process

Lab|Process development

Lab|Process

Swelling factor

~1.7

Stability

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

StandardComponents

MSTFA (N-Methyl-N-trimethylsilyltrifluoroacetamide)

Storage Conditions

At room temperature. Precipitation can occur at low temperature.

20% ethanol at 4Â°C to 30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

Store at room temperature.

pH Stability Cleaning-in-Place (CIP)

1 to 14

Pictogram

GHS08

GHS07

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Stokes Radius

~ 10

System

Sepax C-Pro|Sefia|Sepax 2

Identification

Pass Test

Theoretical Plates

> 43 000 m^-1

5000 m-1

> 42 000 m^-1

> 48 000 m^-1

> 13000 m-1

Synonym

DMSO

Sterilization

0.1 Âµm sterile filtered

Purity

>=99%

Water

0.04% max.

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

SMILES

CS(=O)C

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

WGK

nwg

Vapor Density

<1 (vs air)

>1 (vs air)

Viscosity Max

Wetted Part

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Well Volume

8.5

Pack Size

Packaging

Amber Glass

Percent Purity

≥99.9%

7.0 to 7.4

7.2 - 7.5

7.2 pH

7.5 pH

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

RTECS Number

VV7310000

VV7315000

Viscosity

1.98 mPa/s at 25Â°C

Standard Concentration

98 to 100%

Titratable Acid

0.001mEq/g max.

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