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Chromatography

Chromatography Consumables

Reagents and Buffers

Chromatography Media

$0~$10(0)
$10~$25(0)
$25~$50(182)
$50~$75(52)
$75~$100(17)
$100~$200(73)
$200~$300(53)
$300~$400(21)
$400~$500(27)
$500~$750(40)
$750~$1000(29)
$1000~$5000(99)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Application_4

Chromatography system|Manual

Cell isolation

Chromatography system

Applications

ion chromatography: suitable

Application_5

Chromatography system|Manual

Bed Dimensions

16 x 30 mm

16 x 25 mm

26 x 600 mm

7 x 25 mm

10 x 100 mm

6.4 x 100 mm

16 x 100 mm

Assay

99.8%

=99%

Bed Volume

1 mL

320

5 mL

Bed Height

25 mm

100 mm

30 mm

600 mm

Average sample loading capacity

50 mg

400 mg

Assay Percent Range

99.8% min by GC

Capacity

1.2 meq/mL by wetted bed volume

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.7 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.35 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Autoignition Temp

~800 °F

800 °F

Binding Capacity/ml Chromatography Medium

At least 10 mg histidine-tagged protein

> 25 mg horse liver GST/mL resin

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

Bead type

Mag Sepharose Magnetic Beads

Boiling Point

2230 C

189°C

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

CAS_NO

112945-52-5

39339-85-0

9003-70-7

9041-35-4

9050-97-9

112926-00-8

1343-88-0

9004-54-0

104219-63-8

60177-39-1

62610-50-8

65099-79-8

69011-15-0

69011-20-7

7631-86-9

1344-28-1

11128-95-3

126850-14-4

69011-19-4

39389-20-3

63181-98-6

65546-95-4

CAS Registry Number 1

67-68-5

Chem AlertStorageSymbol

Gray

Chemical Stability

Commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

Stable in all commonly used aqueous buffers

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Chromatography technique

Ion Exchange Chromatography

Hydrophobic Interaction Chromatography

Antibody affinity chromatography

GST-tagged protein purification

Affinity coupling

Antibody affinity chromatography.

Group specific affinity

Histidine-tagged protein purification

Column i.d.

6.4

16 mm

26 mm

ChEBI

CHEBI:28262

BioProcess resin

yes

EC Number

215-681-1

238-878-4

215-691-6

231-545-4

Endotoxin Activity Max.

2 EU/ml

0.12 EU/mg

< 0.12 EU/ml

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 mL/min

> 4 ml/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 20 mL/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

< 5 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

2 ml/min - 10 ml/min

Flow Velocity

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 150 cm/h

<15 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 500 cm/h

30-150cm/h

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Format_6

Prepacked column

Prepacked columns

Bulk resin

Chemical Name or Material

Dimethylsulfoxide

Chemical Substance ID

24863799

24865203

24852837

24854823

24854194

24857352

329759181

329759183

329759193

329752001

24852120

24854190

329763461

329759188

329768201

24854645

24857358

24854191

329763462

329752189

329759187

24854187

329768868

329756032

24857354

24867159

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

Color

Colorless to slightly yellow

Gold/Brown

Colorless

White

Column volume

8 mL

1 mL

Composition

nitrogen content, =1.0%

Concentration

1 N in NaOH (Regenerant concentration)

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Density Max.

1.2 g/ml

1.135 g/ml|

1.078 g/ml

Decomposition Information

>190°C

Density

1.100g/cmÂ³

Estimated Shelf Life from Manufacture Date

8 years

3 years

5 years

COLOR

Blue

Empirical Formula

O2Si

MgO3Si

Feature

ferrule type packed column

Flash Point

87°C (188.6°F)

Form

powder or crystals

powder

fine crystals

beads

fine powder

Exclusion Limit Peptides

4 x 10Â³

Formula Weight

78.13

100.39

60.08

Format

matrix material

Formula

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Al2O3

SiO₂

MgO₃Si

Service & Support

O2Si

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Flashpoint (°C)

> 240 C

Grade

high-purity grade (Davisil Grade 633)

for column chromatography

high-purity grade

high-purity grade (Davisil Grade 62)

Standard

high-purity grade (Davisil Grade 646)

high-purity grade (7754)

high-purity grade (Davisil Grade 923)

HPLC

certified reference material

high-purity grade (Davisil Grade 636)

nuclear grade

H-Codes

H371

H372

H319

H373

Health Hazard 2

WARNING!

Grade

Technical Grade

High-purity Grade (Davisil Grade 643)

Group To be Coupled

-NHâ‚‚

Primary amines

Identification

Pass Test

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

IOD-Codes

Ionic Capacity

0.18-0.25 mmol H⁺/ml medium

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

0.29-0.35 mmol Cl^-/ml medium

0.13-0.17 mmol/ml

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Intrinsic Viscosity

~ 17

Ion Exchanger Type

Strong anion exchanger

Weak anion

Weak and strong anion

Weak anion exchanger

Multimodal weak cation exchanger

Strong cation exchanger

Weak cation exchanger

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Alkali-stabilized Protein A-derived (E. coli)

Carboxymethyl

Cibacron Blue

Human IgG

Quaternary amine

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Diethylaminoethyl

Native protein A

Porcine heparin

Recombinant protein A (E.coli)

Sulphopropyl

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Ligand Density

~ 5 mg/mL resin

Approx. 13 Î¼mol/ml

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Connectors for high- and low-pressure chromatography equipment and instructions.

Linear Formula

(CH₃)₂SO

MgO3Si

(C10H12 · C10H10 · C8H8 · C3H9N)X

SiO2

(C10H12 · C10H10 · C8H8)x

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Cross-linked agarose, 4%

agarose, 6%, spherical

4% cross-linked agarose

cross-linked agarose, 4%, spherical

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Highly cross-linked agarose, spherical.

cross-linked, 6% agarose particles

Highly cross-linked agarose

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

2% agarose

Cross-linked dextran

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Ligand Concentration

~25 Âµmol phenyl/mL resin

~45 Âµmol phenyl/mL resin

4.5-8 mg gelatin/ml drained resin

~4mg heparin/mL resin

Ligand_7

Protein L

Butyl, octyl, phenyl

Protein G

Protein A

Protein A, alkali stabilized

MDL Number

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00132722

MFCD00011232

MFCD00145586

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00211881

MFCD03791384

MFCD00132707

MFCD00132771

MFCD00084428

MFCD00132712

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00145841

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

Cyanogen bromide activation

Triazine

Ether linkages and hydrophilic spacer arm

Ether linkages

Epoxy

Maleimide activation

Dextran surface extenders

Reductive Amination

Matrix

4% Agarose

6% Cross-Linked Agarose

Highly cross-linked 4% agarose

Rigid, cross-linked agarose, spherical

Material

Plastic/Stainless Steel

N/A

Melting Point

18.4°C

Molecular Formula

C2H6OS

Molecular Weight

60.08

101.96

0.0

78.129

100.39

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Operating Flow Rate, Max

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

1.0 mL/min

2.6 mL/min

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

Optical Activity

~7 (5% in H 2 O, suspension)

7.0 (5% in slurry)

6.5 (5% in slurry)

Order Information

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

For research use only.

HiLoad columns are prepacked XK columns.

Percoll PLUS is for research use only.

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Pack size

2 x 1 ml 10% slurry

50 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

100 g

5 mL

500 mL

1 L

1.7 mL

100 mL

125 mL

25 g

15 g

1 Ã— 4.7 mL

10 g

2 x 1 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

1.5 g

5 Ã— 1 mL

6 x 1 L

20 mL

300 mL

Partical Size Dry

18 Âµm-111 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>100 Âµm

>20 Âµm

50 Âµm-150 Âµm

Particle size, d_50V

~90 Âµm

34 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~90 Î¼m

~100 Î¼m

~34 Âµm

~34 Î¼m

~75 Âµm

~85 Âµm

~ 8.6 Âµm

~50 Âµm

Osmolality

< 30 mOsm/kg

288-310 mOsm/kg

pH stability, operational

2â€“10

pH 6 to 10

3â€“10

2â€“13

4â€“12

2â€“12

2â€“9

4â€“13

4â€“9

3 to 12

3â€“11

3â€“13

3â€“12

3â€“9

Physical State

liquid

liquidSuspension

solid

Particle Size

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

60 Âµm

24-44 Âµm

60 Î¼m-200 Î¼m

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

Particle size distribution, dry

20 â€“ 80 Âµm

50 â€“ 150 Âµm

Particle size distribution, wet

55â€“165 Âµm

87â€“510 Âµm

15â€“88 Âµm

100â€“300 Âµm

20â€“80 Âµm

90â€“280 Âµm

pH Range

8.9â€“9.9

5.5â€“7.5

8.5â€“9.5

Operating Flow Rate, Recommended

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.5 mL/min

0.50 mL/min

0.75 mL/min

1.3 mL/min

0.30 mL/min

Pore Size

~0.8 cm 3 /g pore volume

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

0.7-0.85 cm 3 /g pore volume (approx.)

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

110 Å pore size

~0.55 mL/g pore volume

0.75 cm 3 /g pore volume

60 Å mean pore size

150 Å

90 Å mean pore size

Pack Size

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/Flow Specification

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Operating pH

0 - 14

0 - 7

Osmolality

322-344 mOsm/kg

< 25 mOsm/kg

276-298 mOsm/kg

Packaging

Amber Glass

Packaging Layout

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

500 g in poly drum

2.5 kg in poly drum

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

2.5, 5, 25 kg in fiber drum

100, 500 g in poly bottle

5, 25 kg in fiber drum

Percent Purity

≥99.9%

Physical Form

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Liquid

P-CODES

P260

P314

P305 + P351 + P338

Particle Size in Methanol Max

163

pH stability, CIP

2â€“10

3â€“14

2â€“13

2â€“13.7

3â€“12

3â€“13

3â€“10

4â€“13

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

1â€“14

2â€“14

2â€“12

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

Quality

monodispersed

wet

regenerated

dry

Quantity

100 g

500 g

50 g

Recommended Flow Rate

1 ml/min

5 ml/min

Resin Type

MabSelect

Sepharose CL-4B

Capto

Sepharose Fast Flow

Sepharose High Performance

pH ligand fully charged

Above 6

< 9

Entire pH range

Below 9

Pictogram

GHS07

GHS08

Refractive Index

1.4770 to 1.4800 (at 25°C)

RTECS Number

VV7310000

VV7315000

S-Codes

26-36

Sample Volume

<= 13 ml

4 to 50 ÂµL

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

400 to 1000 Î¼l

25 to 500 ÂµL

<= 5 ml

100 to 300 Î¼l

Scale

Lab|Process

Lab|Process development

Lab

Lab|Process development|Process

Recommended maximum operational flow velocity

300 cm/h

pH Stability Cleaning-in-Place (CIP)

1 to 14

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

PurityGrade Notes

Mobile phase for HPLC applications

R-CODES

36/37/38

48/20

Particle Size in Methanol Min

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Stability

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable

Residueafter Evaporation

5ppm max.

Storage

2 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2 to 8Â°C, 100% Isopropanol

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% ethanol

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2-8C

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

Surface Area

250-350 m 2 /g

530 m 2 /g

175-225 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

~500 m 2 /g

~30 m 2 /g

550 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

289 m 2 /g

480 m 2 /g

380 m 2 /g

500 m 2 /g

300 m 2 /g

470-530 m 2 /g

Signal Word

Danger

Warning

Properties

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Storage Conditions

20% ethanol at 4Â°C to 30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

4â€“30Â°C

Purity

>=99%

99.8%

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Stokes Radius

~ 10

Swelling factor

~1.7

SMILES

CS(=O)C

Synonym

DMSO

Synonyms

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Silica gel 110 NH2

Amberlite(R) IR120 hydrogen form

Activated magnesium silicate; Magnesium silicate

Silica gel

Theoretical Plates

> 13000 m-1

5000 m-1

> 43 000 m^-1

> 42 000 m^-1

> 48 000 m^-1

Vapor Density

>1 (vs air)

<1 (vs air)

Viscosity

1.98 mPa/s at 25Â°C

System

Sepax C-Pro|Sefia|Sepax 2

IUPAC Name

methylsulfinylmethane

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

WGK

nwg

>1600 C

Well Volume

8.5

Water

0.04% max.

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Titratable Acid

0.001mEq/g max.

Viscosity Max

Wetted Part

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

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