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Cytiva, now with the life sciences business from Pall Corporation, is a global biotechnology leader dedicated to helping customers discover and commercialize the next generation of therapeutics. Cytiva brings dedicated technical expertise and a broad portfolio of tools, technologies, and services that enable the development, manufacture, and delivery of transformative medicines to patients.

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Made in USA

Chromatography

Chromatography Consumables

Chromatograph Columns

Liquid chromatography LC columns

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Application

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Highly suitable for preparative purification of histidinetagged recombinant proteins

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Application_4

Centrifugation

Chromatography system|Manual

Avoid Using

Chelating agents such as EDTA, EGTA , citrate

Oxidizing agents, citrate buffers

Bed Height

300 mm

600 mm

100 mm

25 mm

Bed Height With One Adaptor

579 mm-609 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

225 mm-350 mm

525 mm-650 mm

0 mm-29 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

85 mm-109 mm

Bed Volume

5 mL

120

320

1 ml TALON® Superflow /column

212 mL

~ 2.4 mL

1 mL

Bed Height With Two Adaptors

96 mm-156 mm

85 mm-350 mm

58 mm - 106 mm

680 mm-950 mm

0 to 200 mm

0-280 mm

0 mm-26 mm

158 mm-206 mm

80 mm-350 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

Bed Dimensions

7 x 25 mm

26 mm x 400 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

16 x 100 mm

16 x 600 mm

3.2 x 300 mm

16 x 25 mm

26 x 600 mm

Binding Capacity/ml Chromatography Medium

At least 40 mg histidine-tagged protein/mL resin

~ 13 mg ovalbumin

Up to 20 mg histidine-tagged protein

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

Bed Support

Filter

Net

Frit

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

125 mg human IgG/column

up to 15mg histidine-tagged protein

BioProcess resin

yes

Average sample loading capacity

50 mg

Certificate of Analysis

Yes

Chemical Stability

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin

Stable in aqueous buffers commonly used in Protein A Chromatography

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Conditions where the resin may be operated without significant change in function:
in¢ Trifluoroacetic acid, up to 0.2%
in¢ Isopropanol, up to 5%
in¢ Dithiothreitol (DTT), up to 5 mM
in¢ Acetonitrile, up to 70%
in¢ Formic acid, up to 1

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chromatography technique

Ion Exchange Chromatography

Multimodal ion exchange chromatography

Histidine-tagged protein purification

Antibody affinity chromatography

GST-tagged protein purification

Hydrophobic Interaction Chromatography

Group specific affinity

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Column i.d.

26 mm

3.2

50 mm

10 mm

16 mm

5 mm

Column Capacity

< 1 mg/mL

Column volume

1 mL

Flow Rate

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 20 mL/min

4 mL/min (1 mL), 5 mL/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

> 20 ml/min|> 20 ml/min

1.6 mL/min

0.8-3.9 ml/min

< 15 ml/min

< 15 ml/min|< 15 ml/min

5 ml/min - 20 ml/min

< 20 ml/min|< 20 ml/min

1 mL/min - 4 mL/min

< 1 mL/min

< 2.6 mL/min

1.2 - 2.3

2 ml/min - 10 ml/min

For use with_31

AKTA go protein purification system

Format_6

Prepacked column

Kits

Bulk resin

Prepacked columns

Flow Velocity

â‰¤ 600 cm/h

15 cm/h

< 300 cm/h|< 300 cm/h

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

< 150 cm/h

Up to 40 cm/h

< 300 cm/h

< 500 cm/h|< 500 cm/h

< 450 cm/h

15 cm/h|15 cm/h

< 600 cm/h

For Use With

Connect a syringe to the top of a HiTrap columns.

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

C 10 columns

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

PD-10 Desalting Columns

AKTA lab protein purification system such as AKTA go, AKTA pure and AKTA avant

Hold-up Volume of Membrane

<10 Î¼l

<20 Î¼l

<5 Î¼l

Guidance

For AKTAprime

Application_5

Chromatography system|Manual

Includes

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Ionic Capacity

40-85 Âµmol/ml (Cl^-)

Dimensions

H x W x D: 660 x 860 x 710

Ligand

Recombinant protein A (E.coli)

Glutathione

Alkali-stabilized Protein A-derived (E. coli)

Octylamine

TALONÂ® Superflow Cobalt-IMAC

Ligand_7

Protein A, alkali stablilized

2-mercaptopyridine

Protein L

Protein G

Protein A

Protein A, alkali stabilized

Filtrate Vessel Material

Polycarbonate (PC)

Material

Polycarbonate (PC)

Acetal plastic (POM)

Polypropylene (PP)

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

Matrix

Highly cross-linked agarose

Composite of cross-linked agarose and dextran

6% Cross-Linked Agarose

Highly cross-linked agarose, spherical

cross-linked agarose, 4%, spherical

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Ligand Density

6 mg rProtein A/ml medium

Inlet id

1.0 mm (0.04 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

2 mm (0.08 in)

Order Information

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

HiLoad columns are prepacked XK columns

Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

Outlet id

1.55 mm (0.06 in)

1.0 mm (0.04 in)

0.75 mm (0.029 in)

Pack size

1 Ã— 320 mL

1 Ã— 120 mL

1 piece

20 mL

250 columns

53 mL

10 mL

200 mL

25 mL

4 x 53 mL

4.7 mL

5 Ã— 5 mL

20 columns

30 columns

5 mL

1 Ã— 5 mL

50 Ã— 100 ÂµL

50 columns

7 Ã— 1 mL

100 mL

50 mL

1 kit

10 Ã— 2 mL

5 Ã— 1 mL

pH stability, CIP

2â€“10.5

2â€“14Co²⁺-stripped medium

2â€“13.7

3â€“12

2â€“14

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

Porosity

10 or 80 Âµm

10 Î¼m

7 Î¼m

20 Î¼m

Ion exchanger

S (cation exchanger)

Resin Type

Sepharose High Performance

Capto

Sepharose Fast Flow

MabSelect

Superdex 200 Increase

Sample Volume

<= 1.5 ml

< 100 Âµl

<= 15 ml

<= 5 ml

Up to 8.5 mL

600 Âµl

2.5 ml

< 150 Âµl

100-180 Î¼l

<= 13 ml

4 to 50 ÂµL

< 0.5 mL

< 5 mL

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Operating Flow Rate, Recommended

0.45 mL/min

0.5 mL/min

0.075 mL/min

1.3 mL/min

Operating Temperature

4Â°C-60Â°C

4Â°C-35Â°C

Outer Diameter

10-21 mm

12 or 18 mm

Storage

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4Â°C to 30Â°C, 20% Ethanol

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

4 to 30Â°C, 20% Ethanol (Q, DEAE), 20% Ethanol, 0.2 M Sodium Acetate (S)

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, in 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

+4Â°C to +8Â°C

20 to 25Â°C, Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative

20 to 25Â°C, TE buffer (10 mM Tris/HCl, 1mM EDTA) containing 0.05% Kathon CG/ICP Biocide as preservative.

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

pH stability, operational

3â€“12

3â€“10

4â€“9

3 to 12

3â€“13

Metal Ion Capacity

approx. 15 Âµmol Ni²⁺/ml medium

~15 Âµmol Ni²⁺/ml medium

Pressure Limit

20 bar (2 MPa, 290 psi)

2 MPa

Particle size, d_50V

~85 Âµm

~34 Âµm

~34 Î¼m

~ 9 Âµm

~90 Âµm

pH Stability Working Range

3â€“12

Scale

Lab

Lab|Process development|Process

Lab|Process

Lab|Process development

Operating Flow Rate, Max

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

Pack Size

Particle Size

45 Âµm-165 Âµm

60 Âµm-165 Âµm

60 Î¼m-160 Î¼m

Pressure/Flow Specification

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Storage Conditions

Store at 2Â°C to 8Â°C

4 - 30Â°C

4ËšC to 30ËšC, 20% ethanol

Power Consumption

8 VA

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

pH Stability Cleaning-in-Place (CIP)

1 to 14

Recommended Flow Rate

1 ml/min

Relative Humidity

20%-95% (non condensing)

Typical Pressure Drop Over the Packed Bed

~ 2 MPa

~ 3 MPa

Wetted Materials

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Theoretical Plates

5000 m-1

> 38 000 m^-1

> 13000 m-1

Volume

5-20 ml

2-6 ml

400 Âµl - 2 ml

100-500 Âµl

Volume_28

100-500 Âµl

2-6 ml

400 Âµl - 2 ml

5-20 ml

Total binding capacity

~50 mg hIgG/mL resin

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Wetted materials1

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

Wetted Part

yes

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

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148 results for : Chromatograph Columns : "Cytiva"

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