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Chromatography

Chromatography Consumables

Reagents and Buffers

Specialized HPLC Reagents

Buffers for Protein Methods

Gas Chromatography Derivatization Reagents

Chromatography Media

Concentrated Buffers

Silylation Grade Solvents

Silylation Reagents

$0~$10(0)
$10~$25(3)
$25~$50(186)
$50~$75(53)
$75~$100(17)
$100~$200(89)
$200~$300(58)
$300~$400(28)
$400~$500(30)
$500~$750(47)
$750~$1000(31)
$1000~$5000(106)
$5000~$10000(8)
$10000~$50000(1)
$50000~$100000(0)
$100000~$3000000(0)

Absorbance

0.02 AU max. at 330nm

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Additives

Without: Phenol red

Without: Calcium, magnesium

Without: Calcium, magnesium, phenol red

Application

For purification of DNA from small molecules by gel filtration

Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.

For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

Partial purification of an IgE-stimulating factor from a human T-cell line
Removal of BSA from an HIV-2 virus lysate
Purification of anti-mouse Fc IgG from sheep anti-serum

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Intended for research use only, and shall not be used in any clinical or in vitro procedures for diagnostic purposes

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag II and Twin-Strep-tag

Average sample loading capacity

400 mg

50 mg

Bed Dimensions

16 x 100 mm

7 x 25 mm

10 x 100 mm

6.4 x 100 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

Application_4

Chromatography system

Cell isolation

Chromatography system|Manual

Avoid Using

Not stable below pH 2 nor to strong oxidising agents

Bed Height

30 mm

100 mm

600 mm

25 mm

Bed Volume

320

5 mL

1 mL

Autoignition Temp

~800 °F

800 °F

Applications

ion chromatography: suitable

Assay

99.8%

=99%

Capacity

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

0.6 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

0.8 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

1.3 meq/mL by wetted bed volume

4.7 meq/g by dry weight

1.7 meq/mL by wetted bed volume

Binding Capacity/ml Chromatography Medium

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

> 25 mg horse liver GST/mL resin

At least 10 mg histidine-tagged protein

Buffer

Running Buffers

Binding Capacity/Column

At least 10 mg histidine-tagged protein/ml sedimented medium.

Approx. 40 mg histidine-tagged protein/ml medium

Assay Percent Range

99.8% min by GC

CAS_NO

112945-52-5

9003-70-7

9041-35-4

9050-97-9

112926-00-8

1343-88-0

39339-85-0

15461-27-5

39389-20-3

63181-98-6

65546-95-4

75-09-2

11128-95-3

126850-14-4

9004-54-0

7631-86-9

69011-20-7

104219-63-8

60177-39-1

62610-50-8

65099-79-8

69011-15-0

1344-28-1

69011-19-4

ChEBI

CHEBI:28262

Bead type

Mag Sepharose Magnetic Beads

Chem AlertStorageSymbol

Gray

BioProcess resin

yes

Boiling Point

189°C

2230 C

Certificate of Analysis

Yes

Carbon Content in Dry Residue

0.04-5.5%|0.04-5.5%

Chem Alert Storage Symbol

Gray

Chemical Stability

Commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile, 0.5 M NaOH (only for cleaning-in-place), 2 M NaCl

Stable in all commonly used buffer systems

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 0.1 M acetic acid, 8 M urea, 6 M guanidine HCl, 24 % ethanol

Stable in commonly used aqueous buffers - 1.0 M NaOH, 3 M Ammonium sulphate, 30% isopropanol, 70% ethanol, 10% ethylene glycol, 0.5% SDS, 6 M guanidine-hydrochloride, 8 M Urea

Stable in all commonly used aqueous buffers

Commonly used aqueous buffers, e.g. 1 M acetate, pH 6.0, 0.1 M NaOH, 70% ethanol or 6 M guanidine-hydrochloride for 1 h at room temperature

Commonly used aqueous buffers

Stable in aqueous buffers commonly used in Protein G chromatography, 6 M guanidine hydrochloride pH 4.7, 20 mM sodium phosphate + 1% SDS, 70% Ethanol, 95% Ethanol, 20 mM Sodium phosphate, 100 mM Glycine- phosphoric acid, 6 M Urea, 20% ethanol + 2% hibitan

Stable in most aqueous and organic eluent systems.

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

Stable in commonly used aqueous buffers for size exclusion chromatography, 1.0 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 70 % ethanol, 30 % iso-propanol, 30 % acetonitrile

Commonly used buffers, 0.2 M NaOH, 0.2 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 24 % ethanol, 30 % porpanol, 30 % acetonitrile

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Stable in commonly used aqueous buffers, 1 M NaOH

Chemical Substance ID

329759188

329752001

24854191

24863799

24865203

329752189

24854194

24857352

329759181

24852120

24854190

329763461

329759187

329768868

24852837

24854823

329759183

329759193

329768201

24857354

24867159

24854645

24857358

329763462

24852304

24854187

329756032

Application_5

Chromatography system|Manual

Chemical Name or Material

Dimethylsulfoxide

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Column i.d.

16 mm

26 mm

6.4

Chromatography technique

Hydrophobic interaction chromatography

Affinity coupling

Antibody affinity chromatography.

Group specific affinity

Ion Exchange Chromatography

Histidine-tagged protein purification

Antibody affinity chromatography

GST-tagged protein purification

Concentration

20 X

10 X

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 2 to 8°C.

Color

Gold/Brown

Colorless to slightly yellow

Colorless

White

Chemical/Physical Resistance

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Composition

nitrogen content, =1.0%

Disodium Hydrogen Phosphate, Sodium Chloride

Tris Base, Sodium Chloride

Density

1.100g/cmÂ³

Density Max.

1.135 g/ml|

1.2 g/ml

1.078 g/ml

Column volume

8 mL

1 mL

Content And Storage

Store at room temperature.

Concentration

1 N in NaOH (Regenerant concentration)

CAS Registry Number 1

67-68-5

EC Number

231-545-4

215-691-6

215-710-8

215-681-1

238-878-4

231-717-9

200-838-9

Flow Rate

< 20 ml/min|< 20 ml/min

>60 ml/min|> 60 ml/min

< 1 ml/min|< 1 ml/min

< 2.6 ml/min|< 2.6 ml/min

> 20 ml/min

> 4 ml/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

< 4 mL/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

< 5 ml/min

< 10 ml/minThe pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.|< 10 ml/min å¡«å……åºŠä¸Šæ–¹çš

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

>10 ml/min

>60 ml/min

< 20 mL/min |< 20 mL/min

1-4ml/min

< 4 mL/min |< 4 mL/min

< 5 ml/min|< 5 ml/min

0.8â€“3.9 mL/min

< 20 mL/min

< DarcyÂ´s Law

Recommended: 0.5 to 2.0 mL/min

2 ml/min - 10 ml/min

Flow Velocity

< 130 cm/h|< 130 cm/h

< 300 cm/h

< 50 cm/h

< 60 cm/hour|< 60 cm/å°æ—¶

< 450 cm/h

< 450 cm/h|< 450 cm/h

< 150 cm/h at 25Â°C, HR 16/10 columns, 5 cm bed height

< 500 cm/h

30-150cm/h

< 300 cm/h, using XK 50/30 column with 15 cm bed height

<1800 cm/h

<1800 cm/h|< 1800 cm/h

15 cm/h|15 cm/h

< 130 cm/h

< 600 cm/h|< 600 cm/h

â‰¤ 600 cm/h

< 150 cm/h

<15 cm/h

10 cm/h - 50 cm/h|10 cm/h - 50 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

‰¤ 600 cm/h|‰¤ 600 cm/h

For Use With

GC/MS

Endotoxin Activity Max.

0.12 EU/mg

< 0.12 EU/ml

2 EU/ml

Format_6

Prepacked columns

Prepacked column

Bulk resin

Formula

(C₁₀H₁₂ · C₁₀H₁₀ · C₈H₈ · C₃

Al2O3

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

SiO₂

CH2Cl2

MgO₃Si

Service & Support

ZrOCl₂·xH₂O

O2Si

Decomposition Information

>190°C

Estimated Shelf Life from Manufacture Date

8 years

3 years

5 years

Flashpoint (°C)

> 240 C

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Empirical Formula

MgO3Si

O2Si

For Use With

For all Biacore SPR systems.

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Feature

ferrule type packed column

Flash Point

87°C (188.6°F)

Form

powder

powder or crystals

fine crystals

Liquid

beads

fine powder

Exclusion Limit Peptides

4 x 10Â³

Exclusion Limit

4 x 10³ (for Peptides) 5 x 10³ (small organics)

Format

matrix material

Liquid

Formula Weight

100.39

78.13

60.08

Gel Compatibility

Novex® Tris-Glycine Gels, Tris-Glycine Gels, Precise Tris-Glycine Gels

COLOR

Blue

Gel Type

Tris-Glycine Gel

Grade

technical grade

for column chromatography

high-purity grade

high-purity grade (Davisil Grade 62)

high-purity grade (Davisil Grade 633)

Standard

high-purity grade (7754)

high-purity grade (Davisil Grade 923)

Certified Reference Material

HPLC

Group To be Coupled

-NHâ‚‚

Primary amines

H-Codes

H371

H372

H319

H373

Hazard s

Flammable, Irritant

Health Hazard 2

WARNING!

Grade

High-purity Grade (Davisil Grade 646)

High-purity Grade (Davisil Grade 636)

Nuclear Grade

Reagent Grade

High-purity Grade (Davisil Grade 643)

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Identification

Pass Test

Ion Exchanger Type

Multimodal weak cation exchanger

Strong cation exchanger

Weak anion exchanger

Strong anion exchanger

Weak anion

Weak and strong anion

Weak cation exchanger

Ionic Capacity

0.13-0.17 mmol/ml

0.07-0.09 mmol H⁺/ml medium

0.10â€“0.14 mmol Cl^- / mL resin

0.18-0.24 mmol Cl^-/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

0.11â€“0.16 mmol (Cl^-)/mL resin

3-4 mmol/g

0.18-0.25 mmol H⁺/ml medium

Ligand Coupling Method

Dextran surface extenders

Cyanogen bromide activation

Triazine

Ether linkages

Ether linkages and hydrophilic spacer arm

Epoxy

Maleimide activation

Epoxy activation

Reductive Amination

Ligand Density

~ 7 Î¼mol Cibacron Blue/ml medium

2 mg Protein G/ml medium

Approx. 13 Î¼mol/ml

3 mg GammaBind G, type 3/ml medium

3 mg Protein A/ml medium

~ 5 mg/mL resin

~16-23 Âµmol NHS/ml drained medium

4.5-8 mg gelatin/ml drained resin

Ligand_7

Protein A

Protein A, alkali stabilized

Protein G

Butyl, octyl, phenyl

Protein L

Matrix

Rigid, cross-linked agarose, spherical

Cross-linked agarose, 4%

4% Agarose

4% cross-linked agarose

cross-linked agarose, 4%, spherical

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Composite of cross-linked agarose and dextran

Highly cross-linked 6% agarose

Agarose, 4%

cross-linked agarose, 6%, spherical

Cross-linked copolymer of allyl dextran and N,N'-methylene bisacrylamide.

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

cross-linked, 6% agarose particles

Highly cross-linked agarose

2% agarose

Cross-linked dextran

agarose, 6%, spherical

MDL Number

MFCD00132718

MFCD00132726

MFCD00145831

MFCD03096474

MFCD00145586

MFCD03791384

MFCD00078249

MFCD00132701

MFCD00135579

MFCD00084428

MFCD00132707

MFCD00132771

MFCD00132712

MFCD00011232

MFCD00132705

MFCD00132770

MFCD00148280

MFCD00149897

MFCD00211881

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00132722

MFCD00145841

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

Ion exchanger

S (cation exchanger)

Q (anion exchanger)

Molecular Weight

84.93

78.129

101.96

0.0

100.39

60.08

178.13 (anhydrous basis)

60.08

Operating Flow Rate, Max

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol at room temperature;
0.35 mL/min water at low temperature;
0.15 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.50 mL/min, water at room temperature;
0.75 mL/min, 20% ethanol at room temperature;
0.75 mL/min water at low temperature;
0.35 mL/min 20% ethanol at low temperature

0.15 mL/min, water at room temperature;
0.07 mL/min, 20% ethanol at room temperature;
0.07 mL/min water at low temperature;
0.03 mL/min 20% ethanol at low temperature

< 4 mL/min

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

Osmolality

276-298 mOsm/kg

322-344 mOsm/kg

< 30 mOsm/kg

< 25 mOsm/kg

288-310 mOsm/kg

P-CODES

P305 + P351 + P338

P260

P314

Pack size

1.5 g

5 Ã— 1 mL

6 x 1 L

100 g

5 mL

1000 mL

15 g

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 L

1.7 mL

100 mL

125 mL

25 g

500 mL

10 mL

200 mL

25 mL

4 x 50 mL

500 g

6 x 500 mL

1 piece

20 mL

300 mL

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 columns

6 mL

1 Ã— 4.7 mL

10 g

2 x 1 mL

2 x 1 ml 10% slurry

50 mL

Partical Size Dry

50 Âµm-150 Âµm

>20 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

>100 Âµm

18 Âµm-111 Âµm

Particle size, d_50V

~ 8.6 Âµm

~50 Âµm

~ 9 Âµm

ImpRes = ~ 40 Î¼m, otherwise = ~ 75 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~90 Î¼m

~85 Âµm

~75 Âµm

~90 Âµm

34 Âµm

7.5 pH

7.0 to 7.4

7.2 - 7.5

7.2 pH

pH ligand fully charged

Below 9

Above 6

< 9

Entire pH range

pH stability, CIP

2â€“12

2â€“10

3â€“14

2 (ligand dependent)â€“13 (ligand dependent)

2â€“9

4â€“9

3â€“13

2â€“13

2â€“13.7

3â€“12

1â€“14

2â€“14

3â€“10

4â€“13

pH stability, operational

3 to 12

3â€“11

3â€“13

2â€“13

4â€“12

2â€“9

4â€“13

4â€“9

2â€“12

3â€“12

3â€“9

3â€“10

2â€“10

pH 6 to 10

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Pressure/Flow Specification

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

>480 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

24-49 cm/h, pressure drop cm H2O/bed height = 2, bed height 30 cm, 2.6 cm i.d

Base matrix: 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 60 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, column 5 cm i.d.

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

min 150 cm/h, bed height 10 cm, column 5 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

60-120 cm/h, pressure drop cm H2O/bed height=15, bed height 10 cm, 5 cm i.d.

base matrix 70-140 cm/h, pressure drop cm Hâ‚‚O/bed height=15 cm, bed height 10 cm, 5 cm i.d.

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

>47 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 700 cm/h, 1 m diameter column, 20 cm bed height

min 90 cm/h, bed height 10 cm, column 5 cm i.d.

>11 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

0.3 MPa at 700 cm/h, 1 m diameter column, 20 cm bed height

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Connectors for high- and low-pressure chromatography equipment and instructions.

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

Quality

dry

regenerated

monodispersed

wet

Quantity

500 mL

10 x 1 mL ampules

50 g

100 g

500 g

1 L

Kit Contents

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

Ligand

Alkali-stabilized Protein A-derived (E. coli)

Bovine gelatin

Glutathione and 10-carbon linker arm

N-hydroxysuccinimide (NHS) activated

Phenyl

Native protein A

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Sulphopropyl

Cibacron Blue

Human IgG

Quaternary amine

Diethylaminoethyl

Alkaline-stabilized protein A-derived (E.coli).

Porcine heparin

Recombinant protein A (E.coli)

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Carboxymethyl

R-CODES

48/20

36/37/38

Linear Formula

(CH₃)₂SO

(C10H12 · C10H10 · C8H8 · C3H9N)X

MgO3Si

SiO2

(C10H12 · C10H10 · C8H8)x

Intrinsic Viscosity

~ 17

IOD-Codes

RTECS Number

VV7315000

VV7310000

S-Codes

26-36

Sample Volume

25 to 500 ÂµL

400 to 1000 Î¼l

<= 5 ml

100 to 300 Î¼l

<= 13 ml

4 to 50 ÂµL

0.5 to 1 ml (Gravity), 0.75 to 1.0 ml (Spin)

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Material

PEEK

Plastic/Stainless Steel

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Ligand Concentration

4.5-8 mg gelatin/ml drained resin

~45 Âµmol phenyl/mL resin

~4mg heparin/mL resin

~25 Âµmol phenyl/mL resin

Operating pH

0 - 14

0 - 7

Order code

SH30237.01

SH30588.02

SH30031.FS

SH30031.02

SH30268.01

SH30588.01

SH30031.01

Matrix

Highly cross-linked 4% agarose

Highly cross-linked agarose, spherical.

Standard Concentration

98 to 100%

Surface Area

120 m 2 /g

289 m 2 /g

480 m 2 /g

380 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

175-225 m 2 /g

=480 m 2 /g (approx.)

430-530 m 2 /g

250-350 m 2 /g

530 m 2 /g

550 m 2 /g

~30 m 2 /g

300 m 2 /g

470-530 m 2 /g

~500 m 2 /g

Molecular Formula

C2H6OS

Operating Flow Rate, Recommended

1.3 mL/min

0.50 mL/min

0.5 mL/min

0.30 mL/min

0.04 mL/min

0.075 mL/min

0.80 mL/min

0.75 mL/min

Optical Activity

6.5 (5% in slurry)

7.0 (5% in slurry)

~7 (5% in H 2 O, suspension)

Order Information

This product is part of our Custom Designed media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative. This product is produced based on a customer order. Delivery

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

For research use only.

Percoll PLUS is for research use only.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Tricorn high-performance columns have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Column not suitable for use with AKTAprime plus system.

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

HiLoad columns are prepacked XK columns.

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

IUPAC Name

methylsulfinylmethane

Packaging Layout

2.5, 5, 25 kg in fiber drum

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

2.5 kg in poly drum

500 g in poly drum

100, 500 g in poly bottle

5, 25 kg in fiber drum

10kg packaged in open-head fiber drums; 50g, 250g, 500 g, 1kg packaged in poly bottles

500 g in poly bottle

1 ft3 in fiber drum

25 kg in fiber drum

25, 500 g in poly bottle

Particle Size

24 Âµm-44 Âµm

25 Âµm-75 Âµm

30 Âµm-40 Âµm

45 Âµm-165 Âµm

45 Âµm-165 Î¼m

45 Î¼m-165 Î¼m

24-44 Âµm

60 Âµm

60 Î¼m-200 Î¼m

Particle size distribution, wet

100â€“300 Âµm

20â€“80 Âµm

55â€“165 Âµm

87â€“510 Âµm

15â€“88 Âµm

90â€“280 Âµm

pH Stability Cleaning-in-Place (CIP)

1 to 14

Pictogram

GHS08

GHS07

Pore Size

0.75 cm 3 /g pore volume

~0.8 cm 3 /g pore volume

0.5 mL/g pore volume

0.7-0.9 cm 3 /g pore volume

30 Å

110 Å pore size

0.7-0.85 cm 3 /g pore volume (approx.)

60 Å mean pore size

150 Å

90 Å mean pore size

0.30 mL/g pore volume

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

~0.55 mL/g pore volume

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Melting Point

18.4°C

Material

N/A

PurityGrade Notes

Mobile phase for HPLC applications

Particle size distribution, dry

20 â€“ 80 Âµm

50 â€“ 150 Âµm

pH Range

8.9â€“9.9

8.5â€“9.5

5.5â€“7.5

Pressure/flow characteristics

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20oC using buffers with the same viscosity as water)

Pressure/flow characteristics: â‰¥150 cm/h at <0.1 MPa in a XK 50/30 column with 5 cm diameter and 15 cm bed height (at 20Â°C using buffers with a viscosity similar to water).

Scale

Lab|Process development

Lab|Process

Lab

Lab|Process development|Process

Purity

>=99%

99.8%

Pub Chem CID

679

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Titratable Acid

0.001mEq/g max.

Particle Size in Methanol Max

163

Purity GradeNotes

Mobile phase for HPLC applications

Sanitization Conditions

2% Hibitane/20% Ethanol or with 70% Ethanol

Recommended Flow Rate

1 ml/min

5 ml/min

WGK

nwg

p H

8.5 pH

7.5 pH

Product Line

Pierce™

Pierce™, Tween™

Pierce

Pierce, Tween

>1600 C

Particle Size in Methanol Min

Storage Conditions

20% ethanol at 4Â°C to 30Â°C

4â€“30Â°C

4 - 8Â°C, 20% Ethanol, 10% medium slurry

4ËšC to 30ËšC, 20% ethanol

Store at room temperature.

At room temperature. Precipitation can occur at low temperature.

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

Resin Type

Capto

Sepharose Fast Flow

MabSelect

Sepharose CL-4B

Sepharose High Performance

RIDADR

NONH for all modes of transport

SMILES

CS(=O)C

Pack Size

Packaging

Amber Glass

Storage

2-8C

2 to 8Â°C, Solution of 0.1 M KH2PO4 and 20% Ethanol; 0.1 M Potassium Phosphate containing 20% Ethanol

2Â° to 8Â° C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, 20% Ethanol, 0.05 M Sodium Acetate

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8.0

2Â°Câ€“8Â°C, 20% ethanol

2 to 8Â°C, 100% Isopropanol

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

2ËšC to 8ËšC, 20% ethanol

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

2 to 8Â°C

2 to 8ÂºC, 20% Ethanol, 50 mM Kâ‚‚HPOâ‚„, 1mM MgClâ‚‚ pH 7.2 in 20% Ethanol

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

Percent Purity

≥99.9%

Physical State

liquidSuspension

Solid

liquid

Sterilization

0.1 Âµm sterile filtered

Stability

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Recommended Cleaning-in-Place Reagent

2 M NaCl solution

Residueafter Evaporation

5ppm max.

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Shelf Life

24 months

12 Months

Signal Word

Danger

Warning

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Synonyms

Amberlite(R) IR120 hydrogen form

Dowex(R) 1X8-200 Ion Exchange Resin

XAD16N

Silica gel 110 NH2

Activated magnesium silicate; Magnesium silicate

Silica gel

Silica, fumed; Silica; Silicic anhydride; Silicon dioxide; Silicon dioxide amorphous

Theoretical Plates

> 48 000 m^-1

> 13000 m-1

> 43 000 m^-1

5000 m-1

> 42 000 m^-1

Stokes Radius

~ 10

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

StandardComponents

MSTFA (N-Methyl-N-trimethylsilyltrifluoroacetamide)

Refractive Index

1.4770 to 1.4800 (at 25°C)

Swelling factor

~1.7

Stability

Stable

Vapor Pressure

0.55mbar at 20Â°C

17 mmHg ( 20 °C)

Synonym

DMSO

Storage Spec

2°C to 8°C

System

Sepax C-Pro|Sefia|Sepax 2

Vapor Density

>1 (vs air)

<1 (vs air)

Viscosity

1.98 mPa/s at 25Â°C

Viscosity Max

Wetted Part

Well Volume

8.5

Water

0.04% max.

Type of ion exchanger

Strong anion exchanger (quaternary ammonium)

Strong cation exchanger (methyl sulfonate)

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