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Search results for : Liquid chromatography LC columns

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Chromatography

Chromatography Consumables

Chromatograph Columns

Liquid chromatography LC columns

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Application

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

Purification is done under physiological conditions and mild elution preserves the activity of the target protein

GSTrap 4B columns are used for the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins. Mild elution conditions preserve protein antigenicity and function

Highly suitable for preparative purification of histidinetagged recombinant proteins

Designed for use with a syringe, peristaltic pump, or chromatography system

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

They can be used for any DNA greater than 10 bases in length and are therefore highly suitable for the purification of oligonucleotides or very small DNA fragments following synthesis or a labeling reaction

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Application_4

Chromatography system|Manual

Applications

LPLC: suitable

Bed Height With Two Adaptors

85 mm-350 mm

80 mm-350 mm

96 mm-156 mm

0 to 200 mm

0-280 mm

0 mm-26 mm

158 mm-206 mm

680 mm-950 mm

380 mm-650 mm

546 mm-606 mm

650 mm-950 mm

58 mm - 106 mm

Bed Support

Filter

Net

Average sample loading capacity

50 mg

Bed Height

600 mm

100 mm

25 mm

Bed Volume

1 ml TALON® Superflow /column

320

120

5 mL

1 mL

Bed Dimensions

16 x 25 mm

26 x 600 mm

7 x 25 mm

16 x 100 mm

16 x 600 mm

0.7 x 2.5 cm

1.6 x 2.5 cm

Bed Height With One Adaptor

85 mm-109 mm

225 mm-350 mm

525 mm-650 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

579 mm-609 mm

129 mm-159 mm

185 mm-209 mm

825 mm-950 mm

0 mm-29 mm

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

125 mg human IgG/column

Binding Capacity/ml Chromatography Medium

~ 13 mg ovalbumin

Approx. 35 mg human lgG/mL at 2.4 min residence time

Approx. 40 mg histidine-tagged protein

At least 40 mg histidine-tagged protein/mL resin

Avoid Using

Chelating agents such as EDTA, EGTA , citrate

Oxidizing agents, citrate buffers

Application_5

Chromatography system|Manual

BioProcess resin

yes

Capacity

1.1 meq/mL by wetted bed volume

Chromatography technique

Hydrophobic Interaction Chromatography

Histidine-tagged protein purification

Antibody affinity chromatography

GST-tagged protein purification

Group specific affinity

Ion Exchange Chromatography

Chemical Stability

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Stable in commonly used aqueous buffers - 0.1 M HCl, 0.1 M NaOH, 8 M urea

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to acetone, ketones, chlorinated hydrocarbons, aliphatic esters, phenol, NaOH, >10% HCl, >5% acetic acid, or strong mineral acid|å¯èinå—æ¶²ç›¸å±‚æžä½¿ç”¨çš,æ°´æin

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

Commonly used aqueous buffers,1 M sodium hydroxide6, 6 M guanidine hydrochloride, 30% isopropanol, and 70% ethanol

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

For Ni²⁺-stripped medium 0.01 M HCl, 0.1 M NaOH. Tested for one week at 40°C 1 M NaOH, 70% HAc. Tested for 12 h 2% SDS. Tested for 1 h 30% 2-propanol. Tested for 30 min

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Stable in aqueous buffers commonly used in Protein A Chromatography

Certificate of Analysis

Yes

Bore Size (mm)

CAS_NO

69011-20-7

9052-95-3

COLOR

Blue

Column i.d.

5 mm

26 mm

10 mm

16 mm

50 mm

Flow Velocity

< 450 cm/h

â‰¤ 600 cm/h

15 cm/h

< 600 cm/h

< 300 cm/h

< 500 cm/h|< 500 cm/h

15 cm/h|15 cm/h

< 300 cm/h|< 300 cm/h

10 cm/h - 50 cm/h

100 cm/h - 300 cm/h 1

< 150 cm/h

Up to 40 cm/h

Chemical Substance ID

24870506

Flow Rate

0.5 ml/min - 2.5 ml/min

1 mL/min|1 mL/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 2.6 mL/min

1.2 - 2.3

2 ml/min - 10 ml/min

< 4 ml/min

< 4 ml/min|< 4 ml/min

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

< 1 mL/min

< 20 mL/min

4 mL/min (1 mL), 5 mL/min (5ml)

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

> 20 ml/min|> 20 ml/min

1.6 mL/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

0.8-3.9 ml/min

< 15 ml/min

5 ml/min - 20 ml/min

Diameter Metric Outer

32 mm

Column Capacity

< 1 mg/mL

66 mL

Column volume

1 mL

For Use With Equipment

Omnifit EZ and EZ SolventPlus Columns

420570 Series reservoir and 420575 series flow control adapters

Format_6

Kits

Prepacked columns

Bulk resin

Prepacked column

Diameter Metric Inner

26 mm

13 mm

6.6 mm

Filtrate Vessel Material

Polycarbonate (PC)

Description

Omnifit EZ Replacement Glass, 6.6 x 50mm; 1/ea

For Use With

PD-10 Desalting Columns

Connect a syringe to the top of a HiTrap columns.

HiTrap and HiPrep columns (connecting column to AKTAdesign systems).

The volumes are designed for Biacore 8K+, Biacore 8K but the kit can be used with all Biacore SPR systems

For Use With Application

High resolution size exclusion chromatography (SEC) in micro scale (4 to 50μL sample volume)

Hold-up Volume of Membrane

<5 Î¼l

<20 Î¼l

<10 Î¼l

H-Codes

H319

Formula

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

Ligand_7

2-mercaptopyridine

Protein A

Protein A, alkali stabilized

Protein A, alkali stablilized

Molecular Weight

0.0

Inlet id

2 mm (0.08 in)

0.75 mm (0.029 in)

1 mm (0.04 in)

1.0 mm (0.04 in)

1.55 mm (0.06 in)

Order Information

HiLoad columns are prepacked XK columns

Columns included in HiTrap HIC Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

Disposable columns for clean-up of proteins/oligosaccharides. The columns are prepacked with Sephadex G-25 resin and for sample volumes up to 2.5 mL by gravity flow and columns spin flow. PD- 10 Buffer reservoir has to be ordered separately For smaller s

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

HiLoad columns are prepacked XK columns.

Not suitable for use with AKTAprime plus system.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Allows safe, reliable storage and transport of self-packed Tricorn columns.

Pack size

200 mL

25 mL

4 x 53 mL

1 kit

5 Ã— 1 mL

53 mL

1 Ã— 320 mL

1 Ã— 5 mL

50 Ã— 100 ÂµL

50 columns

7 Ã— 1 mL

100 mL

20 columns

30 columns

5 mL

1 Ã— 120 mL

1 piece

20 mL

4.7 mL

5 Ã— 5 mL

Particle size, d_50V

~85 Âµm

~90 Âµm

~34 Âµm

~34 Î¼m

pH Stability Working Range

3â€“12

pH stability, CIP

2â€“14

2â€“13.7

3â€“12

2â€“10.5

Porosity

20 Î¼m

7 Î¼m

10 or 80 Âµm

10 Î¼m

IOD-Codes

Ligand

Octylamine

Recombinant protein A (E.coli)

Glutathione

Alkali-stabilized Protein A-derived (E. coli)

Sample Volume

600 Âµl

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

<= 1.5 ml

<= 13 ml

< 0.5 mL

< 5 mL

2.5 ml

100-180 Î¼l

<= 15 ml

<= 5 ml

Signal Word

Warning

Matrix

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

Highly cross-linked agarose

Highly cross-linked agarose, spherical

cross-linked agarose, 4%, spherical

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Length Metric

50 mm

457 mm

Length Metric

500 mm

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Ligand Coupling Method

Epoxy activation

MDL Number

MFCD00132726

MFCD00146442

Ionic Capacity

40-85 Âµmol/ml (Cl^-)

Length English

18 in.

Material

Polycarbonate (PC)

Glass

Polypropylene (PP)

Stainless Steel

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

Theoretical Plates

5000 m-1

> 13000 m-1

Operating Flow Rate, Recommended

0.5 mL/min

1.3 mL/min

Outlet id

1.0 mm (0.04 in)

1.55 mm (0.06 in)

0.75 mm (0.029 in)

P-CODES

P305 + P351 + P338

Pack Size

pH stability, operational

3â€“13

3â€“10

4â€“9

3â€“12

Pressure Limit

20 bar (2 MPa, 290 psi)

Includes

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

1 Ã— Column stand 4 Ã— PD-10 spin adaptor 1 Ã— Buffer tray 30 Ã— Bottom sleeve Instructions are supplied with the columns

Instructions supplied with the concentractors

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

S-Codes

Storage

+4Â°C to +8Â°C

4 to 30Â°C

4 to 30Â°C, 20% Ethanol, 0.1 M Potassium Phosphate buffer, pH 8

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol (Q, DEAE, ANX,CM), 20% Ethanol, 0.2 M Sodium Acetate (SP)

4Â°C to 30Â°C, 20% Ethanol

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.05 M Sodium Acetate containing 20% Ethanol

4â€“30Â°C, 20% Ethanol

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

2Â°Câ€“8Â°C, 20% ethanol

4 to 30ËšC

4Â°C to 8Â°C

4 to 30Â°C, in 20% ethanol

4 to 30Â°C, 0,05% Kathon CG/ICP Biocide

4 to 30Â°C, 0.15% Kathon CG

4 to 30Â°C, 20% Ethanol or 70% Acetonitrile

Kit Contents

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Quantity

Resin Type

Sepharose High Performance

MabSelect

Sepharose Fast Flow

Ion exchanger

S (cation exchanger)

Size MetricStopcock

2 mm bore

Matrix

6% Cross-Linked Agarose

Particle Size

60 Âµm-165 Âµm

45 Âµm-165 Âµm

Storage Requirements

4° to 30°C

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

PoreSize

40 to 60 µm

Volume_28

400 Âµl - 2 ml

5-20 ml

2-6 ml

100-500 Âµl

Pore Size

40 to 60 µm

Ligand Density

6 mg rProtein A/ml medium

WGK

Type

Chromatography Column

Liquid Chromatography (LC)

Gravity Chromatography Column

RIDADR

NONH for all modes of transport

Volume

5-20 ml

100-500 Âµl

2-6 ml

400 Âµl - 2 ml

Pictogram

GHS07

Pressure/Flow Specification

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

Operating Flow Rate, Max

1.0 mL/min

2.6 mL/min

Operating Temperature

4Â°C-60Â°C

R-CODES

36/37/38

Scale

Lab|Process development

Lab

Lab|Process development|Process

Lab|Process

Storage Conditions

4 - 30Â°C

Store at 2Â°C to 8Â°C

Wetted Part

yes

Recommended Flow Rate

1 ml/min

Volume MetricBed

≈2.4mL

Total binding capacity

~50 mg hIgG/mL resin

Volume Metric Bed

≈2.4mL

If you are viewing this page as a nonregistered user, the price(s) displayed is list price. To view your customer-specific pricing, log in or become a registered user here.

HISTRAP HP 5 X 5 ML

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17524802

5183-4511 | Clear snap cap slit PTFE Si septa 100PK

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HILOAD 26/60 SUPERDEX 200 PG

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StrepTrap HP 5 x 1mL

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Vivaspin 500, 50 kDa MWCO PES

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$218.28 / EA

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HiScale 16 20BH

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$1,342.89 / EA

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XK 16/70 column

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$1,107.07 / EA

28988946

XK 50/30 column

Cytiva

$1,807.56 / EA

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XK 50/100 column

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$2,114.24 / EA

28988965

XK 16/100 column

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$1,203.49 / EA

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XK 16/40 column

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RPROTEIN A SEPHAROSE FF 5 ML

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$853.81 / EA

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HITRAP PHENYL HP, 5 X 1ML

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399 results

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