Government Scientific Source

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Application

Clean screen, Binding level screen, Affinity screen

LMW screen
Antibody/general screen
LMW kinetics and affinity
Antibody/general kinetics

Content AndStorage

Store at room temperature.

Description

B-PER II Bacterial Protein Extraction Reagent (2X)

For Use With

Biacore 8K+
Biacore 8K
Biacore T200
Biacore S200

Biacore X100 and Biacore 3000 SPR systems.

Biacore 8K+
Biacore 8K
Biacore T200
Biacore S200|Biacore 8K+
Biacore 8K
Biacore T200
Biacore S200

Fraction collector F9-T

Flow cell Volume

40 nL

Form

liquid

Dissociation Rate Constant (k_d)

10^-6 to 0.5 s^-1

Flow Rate

1 to 100 Î¼L/min

Height

322 mm

875 mm

In-line Reference Subtraction

Automatic

Injection Volume

1 to 200 Î¼L

Includes

Biacore X100 Plus Package software; in-line degasser; handbook.

Biacore 8K instrument, roller table, waste container, 2 Ã— licenses for Biacore Insight Evaluation Software (including Biacore 8K Control Software), 2 Ã— licenses for Extended Screening and Characterization Extension

Mains Requirement

Processing unit: Autorange voltage 100 to 240 V~, frequency 50/60 Hz

Quantity

250mL

Storage Conditions

Store all products at +2 to 8Â°C except BIAdesorb solution 1, which should be stored at room temperature (SDS precipitates at low temperature).

Product Line

B-PER

Kit Contents

BIAtest solution, with HBS-EP, 65 mL
BIAnormalizing solution, (40% (w/w) glycerol), 30 mL.
BIAnormalizing solution, 30 mL (70% (w/w) glycerol) (to be used with Biacore 3000 SPR system).
BIAdesorb solution 1, 90 mL
BIAdesorb solution

Sample Concentration Min

â‰¥ 1 picomolar (pM)

Pack Size

Molecular Weight Detection

No lower limit for organic molecules

Pack size

1 piece

Sample Volume Min

Injection volume plus 20 to 50 Î¼L (application dependent)

Refractive Index

1.33 to 1.39

Reagent Type

Detergent Solution, Cell Lysis Buffer

Sample Type

Small-molecule drug candidates to high-molecular weight proteins (also DNA, RNA, polysaccharides, lipids, cells, and viruses) in various sample environments (e.g., in DMSO-containing buffers, plasma, and serum)

Power Consumption

Processing unit: max 350 VA

Width

550

902

Weight

127

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