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Accession Number

NP_005347

Auto Restart On Power Failure

Yes

Block Uniformity

Block at 55°C: ±0.3°C

Bottom Style

Conical

Block Description

Four sets of 96 x 0.2 mL tubes or four 96-well plates

Brand

Life Sciences

Axygen®

Twist

Binding Capacity

12 µg Based on specified 15 µL aliquot of supplied magnetic bead suspension = 1.5 mg beads @ 100 mg/mL

Cap Material

Polypropylene

CAS_NO

2304-81-6

149022-20-8

39450-01-6

16373-93-6

163795-75-3

9003-98-9

91079-46-8

9001-15-4

146986-50-7

74804-12-9

9001-12-1

856925-71-8

71519-72-7

50-07-7

9001-45-0

Certificate of Analysis

Yes

Chemical Substance ID

329751672

24894129

24890583

24891422

329798458

329773119

Concentration

10 X

100 mM

>=20?/mg

>160U/mg

10 mM

50 X

1 million copies/microliter

Content And Storage

Contains:
• 10 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 2 x 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 10 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (suffic

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion Green HF Buffer
• 5X Phusion Green GC Buffer
• DMSO
• 50 mM MgCl2 solution

Both Phusion Green HF and GC Buffers provide 1.5

Color

White

Colorless

Brown

Clear

Color of florescence

Far-Red

Orange

Content AndStorage

Contains:
• 1 mL Phusion Blood Direct PCR Master Mix (2X)
• 40 µL Universal Control Primer Mix
• 0.5 mL 50 mM EDTA
• 1.5 mL 50 mM MgCl2
• 0.5 mL DMSO
• 1.25 mL Water, nuclease-free
• 50 µg O'GeneRul

Contains:
• 4 x 1.25 mL DreamTaq Hot Start Green PCR Master Mix (2X)
• 4 x 1.25 mL Nuclease-free water

Store at -5 to -30°C.

Includes:

• 2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration)
• Water, nuclease-free
• DMSO

Store at -20°C.

Contains:
• 2 x 1.25 mL Phire Plant Direct PCR Master Mix (2X)
• 5 mL Dilution Buffer
• 40 µL Control Primer Mix (25 µm each)
• 2 x 1.25 mL Water, nuclease- free
• 50 µg O'GeneRuler Express DNA Ladder (sufficient f

The RevertAid RT Kit contains:
• RevertAid Reverse Transcriptase
• RiboLock RNase

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 ++M Forward GAPDH Primer, 10 ++M Reverse GAPDH

Contains:
• 4 x 1.25 mL DreamTaq Green PCR Master Mix (2X), which includes DreamTaq DNA Polymerase, 2X DreamTaq Green buffer, dNTPs, and 4 mM MgCl2
• 4 x 1.25 mL Nuclease-free water

Store at -20-°C.

• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
• 10 mM dNTP Mix

Store at -5 to -30°C.

Includes:
• Phusion U Hot Start DNA Polymerase, 2 U/µL
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1X concentration.

S

Contains:
• 2 x 1.25 mL Maxima SYBR Green/ROX qPCR Master Mix (2X) containing Maxima Hot Start Taq DNA Polymerase, dNTPs (also dUTP) and SYBR Green I in an optimized PCR buffer with ROX passive reference dye; sufficient for 200 x 25 µL reactions.

G?° 4 x 1.25 mL 2X Phire Hot Start II PCR Master Mix
G?° 1 x 0.5 mL DMSO
G?° 4 x 1.25 mL Water, nuclease free

Store at -20°C.

Includes:
• Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL)
• 5X Phusion HF Buffer
• 5X Phusion GC Buffer
• DMSO
• 50 mM MgCl2 solution
Both Phusion HF and GC Buffers provide 1.5 mM MgCl2 in the final

Capacity

0.2 mL

Country of Origin

CompatibleBuffer

Unique Buffer (10x Buffer SacI)

10x FastDigest Buffer/FastDigest Green Buffer

Unique Buffer (10x Buffer AarI)

10x Buffer Tango

10x Buffer G

Components

20µg Mass spec grade lyophilized Trypsin/Lys-C Mix, 500µL Resuspension Buffer

Depth Cm

Depth In

EC Number

232-585-5

232-667-0

213-488-7

293-434-7

231-791-2

232-606-8

232-582-9

254-457-8

Enzyme

BpiI (BbsI)

Pvu I

Pvu II

AarI

BcuI (SpeI)

BglI

Dpn I

Bgl II

Esp3I (BsmBI)

Mph1103I

Pst I

Sca I

Kpn I

KspAI

LguI (SapI)

Nhe I

Not I

Pfl23II

SacI

Eco31I

EcoR I

Kpn2I

BpiI

Hind III

MssI

AatII

PacI

Psp1406I

Xba I

Xho I

AdeI

Eco147I

Eco81I

PagI

MreI (Sse232I)

Nde I

XmaJI

BshTI

Bst1107I

MunI

Concentration

FidelityvsTaq

2 X

52 X

25 X

52X

Extinction Coefficient in Methanol Max

150000 M-1cm-1

250000 M-1cm-1

Fluorescence Wavelength Max_17

670 nm [m]

570 nm [m]

For Use With Application

Used to lyse E. coli for preparation of plasmid DNA from transformed cell cultures

Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases

Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)

For the routine subcloning of PCR products

In-solution Protein digestion, in-solution digestion of proteolytically resistant Proteins

PCR product analysis

Used to breaks phosphodiester bonds, catalyze the hydrolysis of various Nucleotide polyphosphates

For tissue dissociation combined with other Enzymes such as Elastase, Trypsin/Papain

Format

Solid

Liquid

Formula

C14H21N3O · 2HCl · H2O

C₁₅H₁₈N₄O₅

Na₂O₃PNHC(=NH)N(CH₃)CH₂COOH · 4H₂O

C₃₆H₇₁NO₃

Formula Weight

949.11 Daltons

975.15 Daltons

GCRichPCRPerformance

Medium

Low

High

Gel Compatibility

Acrylamide Gels, Agarose Gels

Expression System

Baculovirus

For Use With Equipment

7000 System, 7300 System, 7700 System, 7900HT System, StepOnePlus System, StepOne System

Export control classification number

EAR99

Form

liquid

DetectionMethod

SYBR

GCRich PCRPerformance

High

Medium

Fidelityvs Taq

1 X

Format

Crystalline powder

Lyophilized powder

Electrical

Universal, 100 to 240V, 50 to 60 Hz

Gene Alias

LCK

Heated Lid Pressure

Adjustable

height inch

Functional Testing

Each batch of PuReTaq Ready-To-Go PCR Beads is tested in a real-time PCR assay to ensure its ability to generate a specific PCR product with Î» DNA; and with negative control samples (untemplated reactions) to ensure the lowest possible levels of either e

Hazardous

Yes

Heated Lid Temperature

35 to 115°C, or off

Hot Start

Built-In Hot Start

Height In

21.0625

GeneID Entrez

3932

Harmonized Code US

3822005090

Includes

2X Phire Hot Start II PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease free; DMSO

Phusion Hot Start II High-Fidelity DNA Polymerase (2 U/µL); 5X Phusion Green HF buffer (provides 1.5 mM MgCl2 in the final 1X concentration); 5X Phusion Green GC buffer (provides 1.5 mM MgCl2 in the final 1X concentration); DMSO; 50mM MgCl2 solution

1.2µg pGEM-T easy vector (50ng/µL), 12µL control insert DNA (4ng/µL), 200µL rapid ligation Buffer (2X), 100U T4 DNA Ligase

2 x 1250µL GoTaq Green MasterMix, 2X, 2 x 1250µL Nuclease free Water, blue dye and a yellow dye, GoTaq DNA Polymerase, dNTPs, MgCl2 and reaction Buffers in a single tube

2X Phusion Hot Start II High-Fidelity PCR Master Mix (provides 1.5 mM MgCl2 in the final 1X concentration); Water, nuclease-free; DMSO

Inhibitors

Gluctathione, Cysteine and Ascorbic Acid

Cysteine, EDTA or o-phenanthroline, alpha2-macroglobulin - a large plasma glycoprotein, alpha2-macroglobulin, alpha1-antitrypsin

Includes LabelorDye

Yes

Item Class Description

Reagents

Height Cm

53.5

Max Gradient C

Max Temperature C

100

MDL Number

MFCD00150192

MFCD00213058

MFCD00005754

MFCD00132129

MFCD00130629

MFCD01095679

MFCD00130876

MFCD08460907

MFCD00084767

MFCD00130830

MFCD02259139

MFCD00130918

MFCD00284715

Kinase Family

SRC Kinase Family

Kit Contents

Bulk first-strand cDNA reaction mixes (5)DTT solutionpd(N)₆ primerNot I-d(T)18 bifunctional primermRNA standardRNase-free water

Room-temperature stable beads containing ~ 2.5 units of recombinant PuReTaq DNA polymerase, dATP, dCTP, dGTP, dTTP, stabilizers, BSA and reaction buffer. When a bead is reconstituted to a 25 Î¼L final volume, the concentration of each dNTP is 200 Î¼M in10

Ready-To-Go GenomiPhi V3 cakes, 1 Ã— 96-well plate; 2x Denaturation buffer, 1 Ã— 1.2 ml; Control DNA (Lambda), 500 ng; 12 Strips of 8 domed caps

Mass

10 µg

Material

Multiple

LabelorDye

Unconjugated

Number of Tests

55 reactions

OptimalReactionTemperature

37° C

Pack size

100 nmol

25 nmol

55 reactions

96 reactions

Packaging

Poly Bottle

Product Cubic inch

401

Product Length (in.)

9.1

Product Line

DreamTaq

FastDigest

Maxima

RevertAid

Phire

Phusion

Product Type

Proteinase K

T4 DNA Polymerase, LIC-Qualified

Phosphodiesterase I

Trypsin/Lys-C Mix

Lysozyme

Collagenase Type 4

MethylationSensitivity

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, dcm methylation-sensitive, CpG methylation-sensitive

Quantity

100 rxn

1000U

200 rxns

100U

4 x 1mL (4 x 100 -µmol of 100mM solution)

1mL (Total: 40-µmol)

1250 x 50++L rxns

20µg

100U (2U/-µL)

100 Units

4 x 0.25mL (4 x 25 -µmol of 100mM solution)

500U (2U/µL)

100 x 50µL rxns

50 Reactions

100mg

200 x 50µL rxns

500U

Quantity

125U

300µL

800µL

25U

800μL (800 reactions)

100 reactions

100µL

100 x 20Î¼L rxns

200U

5 x 1,200U

5 x 1mL

250 x 50Î¼L rxns

50µL

200µL

250U

400µL (400 reactions)

20 reactions

300µL (300 reactions)

3kg

500mg

2,000U

ReactionFormat

Standalone

SuperMix or Master Mix

Separate Components

ReactionSpeed

Fast

Standard

Sample Type

DNA, dsDNA

RNA

Shipping Temperature

-80C

ShippingCondition

Dry Ice

Size

1,000 reactions

100u

20ml

200microl

25ml

500u

50 preps/25 extractors

500 reactions

20 reactions

40 reactions

40µmol each

10µmol each

12.5ml

1 system

1.5ml

10,000u

250 preps

25micromol

1,000 preps

20microg

40micromol

1,000microl

200micromol each

25micromol each

5,000u

100 reactions

200 reactions

50 preps

5ml

9 x 2microg

2,500u

250 preps/100 extractors

Output

USB port

Storage Requirements

−30 to −10°C

-30 to -10°C

4°C

-20°C

<0°C

Overhang

Blunt

3'-A

Physical State

solid

Product Family

Axygen® PCR Strip Tubes

Axygen® PCR Tubes and Caps

Product Size Avg

> 10 kb

Product Type Specs

KOD Hot Start Master Mix

Protein Form

Full Length, Recombinant, Cytoplasmic

Qty/Pk

125 / Pk

1000 / Pk

Quantum Yield in Methanol

> 0.15

> 0.28

Polymerase

Phusion Blood II DNA Polymerase

Phusion Hot Start II DNA Polymerase

Phire Hot Start II DNA polymerase

Phusion U Hot Start DNA Polymerase

Phusion Hot Start II High-Fidelity DNA Polymerase

DreamTaq DNA Polymerase

Taq DNA Polymerase

Source

Crotalus adamanteus venom

Clostridium histolyticum

Tritirachium album

TypeIISRe

Yes

Methylation Sensitivity

Not CpG methylation-sensitive, dcm methylation-sensitive, Not dcm methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, CpG methylation-sensitive

Not dam methylation-sensitive, Not dcm methylation-sensitive, Not CpG methylation-sensitive

Not CpG methylation-sensitive, dam methylation-sensitive, Not dcm methylation-sensitive

Labelor Dye

Unconjugated

Width in

27.5629

Licensing

For licensing information, see the Licensing Statements page in the About Us section.

Min Temperature C

Includes Labelor Dye

Yes

Reaction Format

SuperMix or Master Mix

Refrigerated/Frozen

Frozen

Molecular Weight

269.26

334.33

115000

347.22 (anhydrous free acid basis)

60.6 kDa

0.0

292.33

327.14

507.18 (free acid basis)

565.95

Min Gradient C

Memory

1000 programs max

Kinase Group

Tyrosine Kinases

Label Type

Unlabeled

Max Heating Rate

3.4°C per second

Sterile

One Unit Contains

1 each

OptimalReaction Temperature

37° C

Optimal Reaction Temperature

37° C

PCRMethod

qPCR

Protein Tag

His Tag

Product cubic cm

6564

No ofReactions

200 reactions

100

Order Information

Custom options of the product, including larger pack sizes, are available. Please contact your local sales representative or Technical Support.

Additional pack size available on request, please inquire.

Protein Family

Kinases & Inhibitors

Radioactive

FALSE

PAC code

6814

Power Hz

50/60

Power Vac

100 to 240

SensitivetoHeatInactivation

Yes

System Compatibility

LanthaScreen® Antibody, LanthaScreen® Substrate, Z'-LYTE™, Omnia® Bulk Substrate

Reaction Time

1.5-2 h

Reagent type

Fluorescent Labeling Reagents

Restriction Site

BstZI, NotI and EcoRI

Reverse Transcriptase

RevertAid

Select Product

0.2 ml hinged tube with cap, 96 Reactions

Multiwell Plate, 5 x 96 Reactions

Ship Length (in.)

Shipping Condition

Dry Ice

Approved for shipment on Wet or Dry Ice

Material

Synthetic RNA

Qty/CS

1250 / Cs

10000 / Cs

Number Of Blocks

PAC Code Description

Chemagic Kits MSM I

Research Category

Signal Transduction, Immunology

Other Research

Product Gross weight

14.5

Shipping Weight(lbs)

Ships by Itself

YES

Storage Temperature

-80C

Room Temperature

Storage Buffer

50mM Acetic Acid (pH <3) Resuspension Buffer

Storage Conditions

-15 to -30Â°C

Ambient temperature in pouch with desiccant

Store the kit at ambient temperature. Once opened, completely reseal the pouch containing the Ready-To-Go GenomiPhi V3 DNA amplification kit cakes. To ensure maximum shelf life and optimal performance, storage in a desiccator is recommended. Store recons

Promoter

Sp6 and T7

Product Height (in.)

6.2

SufficientFor

100 reactions

20 reactions

Product Width (in.)

7.1

Protein Subtype

Cytoplasmic Tyrosine Kinases

Species

Human

Purityor QualityGrade

Molecular Biology Grade

Sample Weight

10 ng of genomic DNA; or non-purified cell lysates

Ship Width (in.)

Tag Position

C-Terminal

Product Gross Weight Desc

Source

Egg White

TargetGene

lymphocyte-specific protein tyrosine kinase

Type IISRe

Tests Per Kit

Units Each

1 unit

Width cm

Volume

100 uL

Validated Application

Kinase Assay

UoM Description

kit

Vector

pGEM-T easy vector I

UoM

KIT

Warranty

2 years

Optimal ReactionTemperature

42° C

p H

9.8 to 10.4.5

Primer

Random Primers

Product Weight (lbs)

Reaction Speed

Fast

TargetEntry

LCK

Temperature Accuracy

50°C: ±0.25°C

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