GSTrap 4B Columns, 5 x 5 ml
High binding capacity.
Mild elution conditions preserving protein antigenicity and function.
Easy onestep purifications of Glutathione STransferase (GST) tagged proteins resulting in high purity.
GSTrap 4B are designed to be used with a syringe, pump, or chromatography system, such as ÄKTA design.
GSTtagged proteins can be purified directly from pretreated bacterial lysates using GSTrap 4B. GSTtagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves protein antigenicity and function. If desired, cleavage of the protein from GST can be achieved using a sitespecific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GSTtagged proteins can be detected using colorimetric or immunological methods. The medium, Glutathione Sepharose 4B, is also available as lab packages and is a good choice for scaleup. The columns can be operated with a syringe, peristaltic pump, or liquid chromatography system such as ÄKTA design or FPLC System.