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Temperature

45°C

Absorbance

0.02 AU max. at 330nm

Amount per Unit

4 Pieces

Additives

Without: Calcium, magnesium, phenol red

Without: Calcium, magnesium

Additional Information

Vapor Pressure: 0.55mbar at 20°C

Application

For the purification of GST-tagged proteins from bacterial lysates and other glutathione S-tranferases or glutathione-dependent proteins and for preparation of cytoplasmic extract

HiTrap SP HP and HiTrap Q HP are prepacked, ready to use cation and anion exchange columns for method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

Can be operated with a syringe, peristaltic pump or liquid chromatography system such as AKTA design

For method scouting, group separations, sample concentration and sample clean-up of charged biomolecules

For the removal and/or purification of serine proteases e.g. thrombin

Purification of recombinant proteins tagged with Strep-tag® II and Twin-Strep-tag®

Cell isolation

For screening of selectivity, binding and elution conditions, as well as small scale purifications

Glutathione S-transferase (GST) tagged proteins produced using the pGEX series of expression vectors, Other glutathione S-transferases and glutathione-dependent proteins

Autoignition Temp

800 °F

Bed Volume

5 mL

320

120

1 mL

Applications

HPLC: suitable

solid phase extraction (SPE): suitable

gas chromatography (GC): suitable

LPLC: suitable

UHPLC: suitable

Application_4

Cell isolation

Chromatography system|Manual

Autoclavable

Not Autoclavable

Assay

=99%

Average sample loading capacity

50 mg

400 mg

Bed Height

600 mm

100 mm

25 mm

Bed Support

Net

Filter

Bed Dimensions

1.6 x 2.5 cm

7 x 25 mm

16 x 100 mm

16 x 600 mm

16 x 25 mm

26 x 600 mm

16 x 30 mm

3.2 x 300 mm

Binding Capacity/Column

Approx. 40 mg histidine-tagged protein/ml medium

up to 15mg histidine-tagged protein

Base Style

Flat Bottom

BioProcess resin

yes

Boiling Point

189°C

2230 C

Capacity

1.3 meq/mL by wetted bed volume

1.1 meq/mL by wetted bed volume

1.2 meq/mL by wetted bed volume

=1.1 meq/mL by wetted bed volume

200 µL

0.6 meq/mL by wetted bed volume

1.35 meq/mL by wetted bed volume

1.8 meq/mL by wetted bed volume

=25 % absorption capacity (water)

0.7 meq/mL by wetted bed volume

1.0 meq/mL by wetted bed volume

3.0 meq/g by dry weight

4.7 meq/g by dry weight

1.7 meq/mL by wetted bed volume

Bed Height With Two Adaptors

0 to 200 mm

80 mm-350 mm

0 mm-26 mm

158 mm-206 mm

96 mm-156 mm

Assay Percent Range

99.8% min by GC

Binding Capacity/ml Chromatography Medium

>10 mg/mL

>Approx. 10 mg GST-tagged protein/mL resin

At least 40 mg histidine-tagged protein/mL resin

At least 10 mg histidine-tagged protein

Avoid Using

Oxidizing agents, citrate buffers

Bed Height With One Adaptor

85 mm-109 mm

140-280 mm

230 mm-350 mm

810 mm-950 mm

Base Material

Anhydrous Na2SO4

CarbonLoad

11%

20%

15%

Buffer

Running Buffers

Brand

Axygen®

Application_5

Chromatography system|Manual

Bore Size (mm)

CAS_NO

112945-52-5

112926-00-8

1343-88-0

69011-20-7

9052-95-3

39389-20-3

7631-86-9

9003-70-7

104219-63-8

CAS Registry Number 1

7631-86-9

67-68-5

Certifications/Compliance

Manufactured in cGMP (21 CFR 820) compliant and ISO9001 certified facilities

Chem AlertStorageSymbol

Gray

Certificate of Analysis

Yes

Chem Alert Storage Symbol

Gray

ChEBI

CHEBI:28262

Chemical Stability (Column Hardware)

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents and chlorinated hydrocarbons.

Resistant to most solutions used in liquid chromatography except hydrocarbons, aromatic solvents, and chlorinated hydrocarbons.

Chemical Stability

Stable in aqueous buffers commonly used in protein A chromatography, 8 M urea, 6 M gua-HCl, 2% benzyl alcohol, 10 mM NaOH (pH 11), 0.1 M sodium citrate/HCl (pH 3), 20% ethanol

Commonly used aqueous buffers

Resistant to aqueous solutions and solvents used in liquid chromatography. Not resistant to Acetone, Ketones, Chlorinated Hydrocarbons, Aliphatic Esters and Phenol. Should be avoided: >10% NaOH, >10% HCl, >5% acetic acid, and strong mineral acids.

The columns are designed to be used in aqueous solutions and most organic solvents commonly used in liquid chromatography of biomolecules, with the following few exceptions: chlorinated hydrocarbons, acetone and other ketones, aliphatic esters, and phenol

Stable to commonly used aqueous buffers:1.0 M NaOH, 8 M urea, 8 M guanidine hydrochloride, 70% ethanol

0.01 M HCl and 0.01 M NaOH (one week); 10 mM EDTA, 5 mM DTT, 1 M NaOH, 5 mM TCEP, 20 mM 1²-Mercaptoethanol, and 6 M guanidine-HCl (24 hours); 500 mM imidazole and 100 mM EDTA (2 hours); 30% 2-propanol (20 minutes).

Resistant to most solutions used in liquid chromatography except Hydrocarbons, Aromatic solvents and Chlorinated Hydrocarbons

Chemical Substance ID

24852837

329759181

24870506

329750331

329759187

24857358

329763462

329763461

Chemical Name or Material

Silica Gel

Dimethylsulfoxide

Chromatography technique

Affinity coupling

Ion Exchange Chromatography

Histidine-tagged protein purification

Group specific affinity

Antibody affinity chromatography

GST-tagged protein purification

Hydrophobic Interaction Chromatography

ClosureType

Screw Top

Screw

Snap Cap

Screw cap

Color

Amber

Black

Clear

Brass

White

Yellow

Natural

Red

Gold

Silver

Colorless

Gold/Brown

Chemical/Physical Resistance

Temp. Limits:. -10 C to 180 C isothermal and programmed

Swelling (dry to solvent saturated, %):hexane 10-15; toluene 10-15; ethylene dichloride 15-20; ethylene acetate 30-40; 95% ethylene alcohol 60-70; water 60-70.

Column i.d.

26 mm

10 mm

16 mm

50 mm

5 mm

Column Type

capillary extremely polar

capillary chiral

Mixed Mode

Reversed Phase

capillary polar

capillary non-polar

packed GC

Closure Color

Black

Silver

Closure Type

With Titeseal Open-bottom Plug Style Plastic Closures, Unattached

Crimp Cap

Screw cap

Screw

ClosureMaterial

Polypropylene

Composition

Discovery ® DSC-18, 400 mg

Tris Base, Sodium Chloride

bed wt., 500 mg

magnesium sulfate, 150 mg

Density Max.

1.2 g/ml

Closure Material

Aluminum

Polypropylene

Plastic

Polyethylene

Closure Size

11 mm

13 mm

20 mm

9 mm

Column Format

Analytical Column

Analytical Column, EASY-Spray

Column Capacity

< 1 mg/mL

Column volume

1 mL

8 mL

Color

Colorless to Slightly Yellow

Grey

N/A

Density

1.100g/cmÂ³

Compatibility

Compatible with most QuEChERS shakers.

ClosureSize

11 mm

9 mm

Concentration

20 X

Decomposition Information

>190°C

Content AndStorage

Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, between the following temperatures: 20 to 25°C.

COLOR

Blue

Diameter Metric

75 µm

2.1 mm

Diameter Metric Inner

12 mm

15 mm

6.6 mm

Diameter Metric

4.6 mm

Diameter Metric Outer

8 mm

15 mm

12 mm

Exclusion Limit Peptides

4 x 10Â³

Feature

closure type black phenolic solid top cap

without needle Luer tip

needle type N (cemented)

septum type Thermogreen ® LB-2

Endcapped

syringe type Sample Lock

Not Endcapped

closure type screw top vial

preconditioned

Flow Velocity

< 150 cm/h

< 300 cm/h|< 300 cm/h

< 60 cm/hour

10 cm/h - 50 cm/h

< 75 cm/h at 25 Â°C, HR 16/10 column, 5 cm bed height

15 cm/h

150 cm/h - 600 cm/h|150 cm/h~600 cm/h

<1800 cm/h

15 cm/h|15 cm/h

< 450 cm/h

< 300 cm/h

â‰¤ 600 cm/h

Filtrate Vessel Material

Polycarbonate (PC)

Finish

Plain top

Flow Rate

2 ml/min - 10 ml/min

< 20 mL/min

< DarcyÂ´s Law

5 mL/min|5 mL/min

Recommended: 0.5 to 2.0 mL/min

2 ml/min - 10 ml/min|2 ml/min - 10 ml/min

1.6 mL/min

< 1.0 ml/min

< 10 ml/min|< 10 ml/min

< 31 ml/min

>10 ml/min

< 20 ml/min|< 20 ml/min

1 ml/min - 4 ml/min

4 ml/min (1 ml), 5 ml/min (5ml)|4 ml/min (1 ml), 5 ml/min (5ml)

< 4 ml/min

< 4 ml/min|< 4 ml/min

1 ml/min - 4 ml/min|1 mL/min - 4 mL/min

0.20 µm

0.12 µm

EC Number

215-609-9

200-838-9

231-153-3

215-681-1

238-878-4

231-545-4

262-373-8

Diameter MetricOpening

9 mm

Form

fine powder

Liquid

powder

powder or crystals

fine crystals

Formula

O2Si

(C₁₀H₁₂· C₁₀H₁₀· C₈H₈)_x

[CH₂CH(C₆H₅)]_x[CH₂CH[C₆H₄

Service & Support

MgO₃Si

Diameter Metric Opening

11 mm

For Use With

ELSD, UV Detector

Estimated Shelf Life from Manufacture Date

5 years

FilmThickness

1 µm

Endcapped

Proprietary

Yes

Exclusion Limit Small Organics

5 x 10Â³

Field14

50Â°-65Â°

Endotoxin Activity Max.

0.12 EU/mg

Empirical Formula

MgO3Si

Flow Direction

Supported bidirectionally. Certain applications may require orientation, i.e., vents. Reverse flow only for low pressure applications.

Format

Liquid

matrix material

Format_6

Kits

Bulk resin

Prepacked column

Prepacked columns

Flash Point

87°C (188.6°F)

For Use With

Biacore 4000, Biacore T200, Biacore T100, Biacore 8K, Biacore S200 and Biacore X100

Health Hazard 3

Emergency Overview
Combustible liquid. DMSO readily penetrates skin and may carry other dissolved chemicals into the body. Hygroscopic. Use personal protective equipment. Keep away from open flames, hot surfaces and sources of ignition. Ensure

Height English

1.25 in.

Hold-up Volume of Membrane

<5 Î¼l

<20 Î¼l

<10 Î¼l

Housing

Polypropylene (PP)

Formula Weight

100.39

78.13

60.08

H-Codes

H315-H319-H335

H372

H371

H317

H319

H373

H351

Height English

1.77 in.

1.57 in.

Identification

Pass Test

Health Hazard 2

WARNING!

Ion Exchanger Type

Strong cation exchanger

Weak anion exchanger

Weak and strong anion

Weak cation exchanger

Strong anion exchanger

Weak anion

Length Metric

50 mm

60 m

750 mm

250 mm

30 m

Ligand Density

2 mg Protein G/ml medium

~ 5 mg/mL resin

3 mg GammaBind G, type 3/ml medium

Approx. 13 Î¼mol/ml

6 mg rProtein A/ml medium

Ligand_7

Protein A

Protein A, alkali stabilized

Protein L

Protein G

Material

polypropylene hardware

polypropylene body

red PTFE/silicone septum

Stainless Steel Hardware (Column)

Tedlar ® PVDF film

white PTFE/silicone

white rubber liner

Brass

PEEK

Phenolic

Polyetheretherketone (PEEK) Polytetrafluoroethene (PTFE)

polypropylene tube

clear glass

Plastic/Stainless Steel

PTFE

silicone body

stainless steel TD tube (includes screens)

bevel tip stainless steel needle (point style 2)

Cellulose

polypropylene material (hardware)

PTFE-faced Rubber Liner

PTFE/silicone septa

Silica Gel

Stainless Steel

Stainless Steel Hardware

VG2, 60% Vespel/40% Graphite

Glass

glass body

PE frit (20 µm porosity)

clear glass vial

Glutathione Sepharose 4 B

Mixed

PTFE/silicone septum

glass TD tube

Polycarbonate (PC)

SilTite

N/A

Borosilicate glass

Polypropylene

Polypropylene (PP)

PTFE frit

Material Septa

PTFE/Silicone

PTFE/Silicone/PTFE

Matrix

Rigid, cross-linked agarose, spherical

Max Pressure

17400 psi (1200 bar)

900 psi

2000 psi

11600 psi (800 bar)

4500 psi (310 bar)

MDL Number

MFCD00132726

MFCD00145831

MFCD00145842

MFCD00165824

MFCD00166483

MFCD00132722

MFCD00084428

MFCD00132707

MFCD00132771

MFCD00078249

MFCD00146442

MFCD00145841

MFCD00011232

Metal Ion Capacity

~15 Âµmol Ni²⁺/ml medium

54 to 70 Âµmol Ni²⁺/ml medium

approx. 15 Âµmol Ni²⁺/ml medium

Molecular Formula

O2Si

C2H6OS

Molecular Weight

84.93

78.129

60.08

178.13 (anhydrous basis)

0.0

100.39

Operating Flow Rate, Max

1.2 mL/min, water at room temperature;
0.60 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.60 mL/min water at low temperature;
0.30 mL/min 20% ethanol or 10% glycerol at low temperature

1.80 mL/min, water at room temperature;
0.90 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.90 mL/min water at low temperature;
0.45 mL/min 20% ethanol or 10% glycerol at low temperature

< 4 mL/min

0.75 mL/min, water at room temperature;
0.35 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.35 mL/min water at low temperature;
0.17 mL/min 20% ethanol or 10% glycerol at low temperature

1.60 mL/min, water at room temperature;
0.80 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.80 mL/min water at low temperature;
0.40 mL/min 20% ethanol or 10% glycerol at low temperature

0.15 mL/min, water at room temperature;
0.075 mL/min, 20% ethanol or 10% glycerol at room temperature;
0.075 mL/min water at low temperature;
0.035 mL/min 20% ethanol or 10% glycerol at low temperature

1.0 mL/min

2.6 mL/min

Operating Pressure Max

4.1 bar (60 psi)

Order Information

Columns included in HiTrap IEX Selection Kit are available individually as 5 x 1 mL and 5 x 5 mL packages.

The use of these products in Biacore systems requires an Amine Coupling Kit with Sensor Chip CM5, CM4, CM3, or C1.

HiLoad columns are prepacked XK columns. This product is based on customer order. Delivery time can be longer than for standard products.

For research use only.

Not for use with ultrasalt-sensitive automated DNA Sequencers (e.g. ALFexpress II and ABI 377 model.)

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented. RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instruction

HiLoad columns are prepacked XK columns.

Percoll is for research use only.

Tricorn have Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, RESOURCE columns are supplied with connectors for high- and low-pressure chromatography equipment and instructions

Use 20-40 mM imidazole in sample and binding buffers for highest purity.

Percoll PLUS is for research use only.

This product is produced based on a customer order. Delivery time may be longer than for standard products.

Osmolality

276-298 mOsm/kg

< 30 mOsm/kg

P-CODES

P305 + P351 + P338

P260 - P264 - P270 - P308 + P311 - P405 - P501

P280

P314

Pack size

1 kit

1.5 g

10 Ã— 2 mL

5 Ã— 1 mL

6 x 1 L

2 x 1 ml 10% slurry

50 mL

1 L

1 Ã— 5 mL

1 mL

150 mL

250 mL

50 Ã— 100 ÂµL

50 columns

6 mL

7 Ã— 1 mL

1000 mL

10 mL

200 mL

25 mL

500 g

6 x 500 mL

1 Ã— 1 mL

5 Ã— 5 mL

6 x 100 mL

75 mL

1 Ã— 4.7 mL

2 x 1 mL

500 mL

100 mL

1 piece

20 mL

300 mL

100 g

5 mL

Pack Size

PackingMaterial

Mxt-502.2

Partical Size Dry

50 Âµm-150 Âµm

18 Âµm-111 Âµm

20 Âµm-50 Âµm

40 Âµm-120 Âµm

Particle Size

40 to 60 µm

60 Î¼m-160 Î¼m

3 µm

24 Âµm-44 Âµm

60 Âµm-165 Âµm

45 Âµm-165 Âµm

45 Î¼m-165 Î¼m

24-44 Âµm

2 µm

Particle size, d_50V

~ 8.6 Âµm

~50 Âµm

~90 Âµm

34 Âµm

~100 Î¼m

~34 Âµm

~34 Î¼m

~85 Âµm

~ 9 Âµm

~75 Âµm

~90 Î¼m

7.5 pH

7.0 to 7.4

pH ligand fully charged

Below 9

Above 6

Entire pH range

pH stability, CIP

2â€“12

2â€“9

1â€“14

2â€“14

3â€“10

4â€“13

2â€“13

2â€“13.7

3â€“12

2â€“14Co²⁺-stripped medium

2â€“10

3â€“14

pH stability, operational

3 to 12

3â€“11

3â€“13

2â€“10

pH 6 to 10

3â€“10

2â€“9

4â€“13

4â€“9

2â€“12

3â€“12

3â€“9

2â€“13

4â€“12

Physical Form

Liquid

Colloidal solution of silica coated with polyvinylpyrrolidone (PVP)

Colloidal solution of silica particles coated with silane

Spray-dried powder

Polarity

Non-Polar

Pore Size

1000 Å

110 Å pore diameter

60 Å pore size

1 Âµm

100 Å

110 Å pore size

0.8 cm 3 /g pore volume

60 Å average pore size (52-73 Å)

0.75 cm 3 /g pore volume

180 Å

60 +?

60 Å mean pore size

70 Å pore size

~0.55 mL/g pore volume

120 Å

90 Å

0.7-0.85 cm 3 /g pore volume (approx.)

0.7-0.9 cm 3 /g pore volume

30 Å

300 Å

90 Å pore size

100 Å

150 Å

90 Å mean pore size

160 Å

PoreSize

10 µm and 30 µm

120 Å

300 Å

Pressure/Flow Specification

>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.

150 cm/h (at < 3 bar, 25Â°C, 10 cm bed height)

500 cm/h, AxiChrom 300, bed height 20 cm, operating pressure < 2 bar

Base matrix: 250-400 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300 kPa at 600 cm/h, 1 m diameter column, 20 cm bed height

25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.

base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm

300-600 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

400-700 cm/h, 100 kPa, XK 50/30 column, bed height 15 cm

Product Line

nanoLC

Environmental Express

Surestop

Easy Spray

Pierce

ProductLine

Acclaim

TraceGOLD

Properties

Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been

Ficoll-Paque PLUS is a sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA. The density has been optimized for the isolation of human lymphocytes from peripheral blood.

Solution

Quality

dry

monodispersed

with moisture indicator free of heavy metals

Grade

high-purity grade (Davisil Grade 643)

HPLC

Standard

certified reference material

high-purity grade (Davisil Grade 636)

nuclear grade

reagent grade

high-purity grade (Davisil Grade 923)

technical grade

Quantity

1/Pk.

1000

1000/Cs.

100 g

1000/Pk.

100/Pk.

Quantity

500 mL

Each

144 Pack

100

200

100 Pack

R-CODES

48/20

36/37/38

Resin Type

Superdex 200 Increase

MabSelect

Capto

Sepharose Fast Flow

Sepharose High Performance

RTECS Number

VV7315000

S-Codes

26-36

Sample Volume

0.1 to 0.5 ml (Gravity), 0.2 to 0.5 ml (Spin)

25 to 500 ÂµL

<= 13 ml

4 to 50 ÂµL

< 0.5 mL

< 5 mL

600 Âµl

<= 15 ml

<= 5 ml

<= 1.5 ml

Height Metric

40 mm

Height Metric

45 mm

43 mm

32 mm

Sorbent

HyperSep Florisil

Standard Concentration

98 to 100%

Grade

High-purity Grade (Davisil Grade 633)

For column chromatography

High-purity Grade

High-purity Grade (Davisil Grade 62)

Graduations

0.5, 1, 1.5 mL

Group To be Coupled

Primary amines

-NHâ‚‚

Surface Area

120 m 2 /g

289 m 2 /g

450 m 2 /g

480 m 2 /g

~500 m 2 /g

100 m 2 /g

500 m 2 /g

500 m 2 /g (approx.)

800 m 2 /g

250-350 m 2 /g

530 m 2 /g

=480 m 2 /g (approx.)

200 m 2 /g

430-530 m 2 /g

475 m 2 /g

80 m 2 /g

450 m²/g

100 m²/g

22 m 2 /g

320 m 2 /g

450 m 2 /g

300 m 2 /g

470-530 m 2 /g

300 m²/g

170 m 2 /g

Graduations

0.5 mL

Graduated

Guidance

For AKTAprime

Synonyms

14-815-571

SUPELCOGEL(TM) 8% Crosslinked HPLC Column

QuEChERS; QuEChERS

Tedlar(R) Gas Sampling Bag

XAD16N

Activated magnesium silicate; Magnesium silicate

QuEChERS

Silica gel

Temperature Range

-60°C to +340/360°C

Titratable Acid

0.001mEq/g max.

Type

Crimp or Snap Closure

HPLC Column

Analytical Column

Liquid Chromatography (LC)

VialType

9 mm SureStop Vial (AVCS)

11 mm Crimp Top Vial

9 mm Target DP Vial

Volume

22 mL

250 µL

3 mL

50 mL

6 mL

1 mL

0.5 mL

2-6 ml

100-500 Âµl

5-20 ml

2 mL

25 mL

400 Âµl - 2 ml

Volume Metric Working

250 µL

1.8 mL

Volume MetricResidual

<6 µL

<170µL

Volume MetricUsable

4 mL

1.5 mL

Volume Range

0.5 - 200 µL

Volume_28

5-20 ml

400 Âµl - 2 ml

2-6 ml

100-500 Âµl

Water Flow Rate at 10 bar (145 psi)

Wetted Materials

Polyetheretherketone (PEEK)/Polytetrafluoroethylene (PTFE)

WGK

nwg

InChIKey

IAZDPXIOMUYVGZ-UHFFFAOYSA-N

Inlet Connector

Stepped barb for 10 to 12 mm (3/8 to 1/2") tubing

Inlet id

0.75 mm (0.029 in)

1 mm (0.04 in)

1.55 mm (0.06 in)

1.0 mm (0.04 in)

Insert Type

350µL Fused Insert

250µL Fused Insert

Pre-Slit

IOD-Codes

Ligand

Alkaline-stabilized protein A-derived (E.coli).

Octylamine

Carboxymethyl

Native protein A

TALONÂ® Superflow Cobalt-IMAC

Calmodulin

Recombinant GammaBind G type 3 lacking albumin-binding region

Strep-Tactin XT

Alkali-stabilized Protein A-derived (E. coli)

Diethylaminoethyl

Sulphopropyl

Recombinant protein A (E.coli)

Glutathione

Recombinant protein G lacking albumin-binding region, produced in E. coli

Cibacron Blue

Quaternary amine

Glutathione and 10-carbon linker arm

Phenyl

Includes

Two (0.5 m and 2 m L) 1/16 in. OD PTFE tubing assembly with 1/4-28 UNF PP fitting at one end, a frit kit (contains two each of 10 µm PE, 10 µm PTFE, and 30 µm PTFE frits), and a Click-N-Seal 10-32 PEEK conical port fitting.

Vials and Closures

Fritted disc, 2mm bore PTFE stopcock plug and 24/40 standard taper upper joint

100 vials and 100 seals with septa

MarkingSpot

Yes

Matrix

Cross-linked agarose, 6%

Highly cross-linked agarose, 6%

Highly crossed-linked agarose, 6%

agarose, 6%, spherical

6% cross-linked agarose

Composite of cross-linked agarose

Highly cross-linked agarose with dextran surface extender

Highly cross-linked agarose, spherical

Hydroxypropylated, cross-linked dextran

Cross-linked agarose, 4%

Agarose, 4%

cross-linked agarose, 6%, spherical

Composite of cross-linked agarose and dextran

Highly cross-linked 4% agarose

cross-linked, 6% agarose particles

Highly cross-linked agarose

Cross-linked dextran

cross-linked agarose, 4%, spherical

Ionic Capacity

0.18-0.25 mmol H⁺/ml medium

0.18-0.24 mmol Cl^-/ml medium

0.29-0.35 mmol Cl^-/ml medium

0.09-0.13 mmol H⁺/ml medium

0.14-0.20 mmol Cl^-/ml

0.16-0.22 mmol Cl^-/ml medium

0.15-0.20 mmol H⁺/mlÂ

40-85 Âµmol/ml (Cl^-)

0.11â€“0.16 mmol (Cl^-)/mL resin

Maximum pressure over packed bed

4.0 MPa (40 bar, 580 psi)

Includes

Dismantling tool, support screen, net ring (10 Î¼m), O-ring, stop plugs, and instructions.

Dismantling tool, support screen, net ring (10 Âµm), O-ring, stop plugs, and instructions.

Connectors for high- and low-pressure chromatography equipment and instructions.

Column valve V9-C
PEEK tubing i.d. 0.75 mm/ o.d. 1/16''
PEEK tubing i.d. 0.5 mm/o.d. 1/16''

Valco fittings for AKTAdesign systems and are supplied with M6 connectors for FPLC System.

2 Ã— 100 ml phosphate 8x stock solution (pH 7.4), and 100 ml highly pure 2 M imidazole (pH 7.4)

Instructions supplied with the concentractors

Kit Contents

Aprotinin (10 mg): 6500 M_r
Ribonuclease A (50 mg): 13 700 M_r
Carbonic anhydrase (15 mg): 29 000 M_r
Ovalbumin (50 mg): 43 000 M_r
Conalbumin (50 mg): 75 000 M_r
Blue Dextran 2000

BIAdesorb solution 1, 500 mL
BIAdesorb solution 2, 500 mL

RNAspin Lysis Solution (Red sticker): 25 ml, RNAspin Wash Buffer I (Yellow sticker): 15 ml, RNAspin Wash Buffer II (Grey sticker): 12.5 ml Add 50 ml Ethanol; RNAspin Desalting Buffer (Blue sticker); 25 ml, RNAspin Desalting Reaction Buffer (Green sticker)

Anti-histidine antibody: 1 mg/ml in 0.15 M NaCl, 90 Î¼l
Immobilization buffer: 10 mM sodium acetate pH 4.5, 2.6 ml
Regeneration solution: 10 mM glycine-HCl, pH 1.5, 2 Ã— 120 ml

Length Metric

150 mm

100 mm

51 mm

Ligand Concentration

~25 Âµmol phenyl/mL resin

~45 Âµmol phenyl/mL resin

Liner Material

PTFE/Silicone

None

Marking Spot

Ligand Coupling Method

Ether linkages

Ether linkages and hydrophilic spacer arm

Maleimide activation

Epoxy activation

Cyanogen bromide activation

Linear Formula

(C10H12 · C10H10 · C8H8 · C3H9N)X

MgO3Si

(CH₃)₂SO

(C10H12 · C10H10 · C8H8)x

Max Temperature

330/350°C for Standard Columns; 430°C for Metal Columns

340/360°C

40°C

Max Temperature English

122°F

Licensing

For licensing information, see the Licensing Statements page in the About Us section. https://www.gelifesciences.com/legal/licensing-statements

For licensing information, see the Licensing Statements page in the About Us section.

Max Temperature

445°C

Material

100% Dimethyl Polysiloxane

Fused Silica

Stainless Steel Column

Operating pH

0 - 9

0 - 7

2 - 7.5

3.5 - 7.0

3.8 - 6.8

1 - 9

2 - 9

2 - 8

0 - 14

Optical Activity

~7 (5% in H 2 O, suspension)

6.5 (5% in slurry)

7.0 (5% in slurry)

Order code

SH30031.01

Packing Material

Spherical, Fully Porous, Ultrapure Silica

Spherical, Nanopolymer Hybrid Silica

Physical State

liquid

liquidSuspension

solid

Pressure/flow characteristics

150-250 cm/h at <0.1MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20Â°C using buffers with the same viscosity as water)

250-400 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

Pressure/flow characteristics: 150-250 cm/h at <0.1 MPa in a XK 50/60 column with 5 cm diameter and 25 cm bed height (at 20â°C using buffers with the same viscosity as water).

No per Pack

100

Operating Flow Rate, Recommended

0.50 mL/min

0.075 mL/min

0.80 mL/min

1.3 mL/min

0.5 mL/min

Operating Temperature

4Â°C-35Â°C

Osmolality

< 25 mOsm/kg

288-310 mOsm/kg

Packaging Layout

1 kg in poly bottle

10, 50, 100, 500 g in poly bottle

1, 6x1, 2.5 kg in poly bottle

2.5, 5, 25 kg in fiber drum

100, 500 g in poly bottle

1 ft3 in fiber drum

25, 500 g in poly bottle

Pictogram

GHS07

GHS08

Preslit

Yes

Ion exchanger

Q (anion exchanger)

S (cation exchanger)

Length English Needle

2 in.

Intrinsic Viscosity

~ 17

IUPAC Name

methylsulfinylmethane

Needle Point Style

Blunt

p H

4 to 13

7.5 pH

2 to 8

2.5 to 7

Refractive Index

1.4770 to 1.4800 (at 25°C)

Packaging

Amber Glass

Percent Purity

≥99.9%

pH Range

5.5â€“7.5

8.9â€“9.9

8.5â€“9.5

Septum

Red PTFE/White Silicone

ParticleSize

3 µm

5 µm

pH Stability Cleaning-in-Place (CIP)

1 to 14

Purity

>=99%

Solubility Information

Solubility aq. soln.: insoluble.

Sterilization

May be autoclaved at 121Â°C for 20 minutes. Multiple autoclave cycles are possible. However, the responsibility for reuse is with the operator. The device should be protected from cross contamination. An integrity test should be performed after autoclavin

0.1 Âµm sterile filtered

Storage

4 to 30Â°C

4 to 8Â°C, 100% Isopropanol

4 to 8Â°C, 20% Ethanol

2-8C

4Â°C to 8Â°C

4 to 30Â°C, 0.15% Kathon CG/ICP Biocide

4 to 30Â°C, dry resins. Used resins 4 to 8 Â°C in 20% Ethanol or 0.01 M NaOH

2 to 8Â°C, 20% Ethanol

4 to 30Â°C, 0.2 M Sodium Acetate in 20% Ethanol

4 to 30Â°C, 20% Ethanol

2Â°Câ€“8Â°C, 20% ethanol

2 to 8Â°C, 0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol

2Â°C to 8Â°C in 20% ethanol

15Â° to 30Â° C

2 to 8Â°C, 20% Ethanol (alternative storage solution 2% benzyl alcohol)

4 to 30Â°C, 20% Ethanol + 0.2 M Sodium Acetate

4Â°C to 30Â°C, 20% Ethanol

Melting Point

18.4°C

Storage Conditions

4â€“30Â°C

4ËšC to 30ËšC, 20% ethanol

At room temperature. Precipitation can occur at low temperature.

4 - 30Â°C

Sealing

Heat-fused

Septum

None

No per Pack

100

SMILES

CS(=O)C

Particle Shape

Spherical

Particle Size in Methanol Min

Outlet id

1.55 mm (0.06 in)

1.0 mm (0.04 in)

0.75 mm (0.029 in)

Phase

Reversed Phase and Ion Exchange

Reversed Phase

Porosity

20 Î¼m

10 or 80 Âµm

7 Î¼m

10 Î¼m

Stability

Percoll PLUS is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Ficoll-Paque PLUS is provided as sterile solution which is stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PLUS product is indicated by the appearance of a yellow color or particulate mat

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4Â°C and 30Â°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color

Percoll is provided as solution which is stable for at least 5 yr when stored between 4Â°C and 30Â°C

Stationary Phase

C18

Reversed Phase/Anion Exchange/Cation Exchange

Particle Size in Methanol Max

163

Pressure Limit

20 bar (2 MPa, 290 psi)

Type of ion exchanger

Strong cation exchanger (methyl sulfonate)

Strong anion exchanger (quaternary ammonium)

Vent

On inlet

Volume MetricTotal

2 mL

Pub Chem CID

679

Purity GradeNotes

Mobile phase for HPLC applications

Recommended Flow Rate

1 ml/min

>1600 C

Reactivity and Stability

The reactivity and stability of Ficoll PM400 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevate

Relative Viscosity

5 Î·r at 20Â°C: 10% (w/v) 20 Î·r at 20Â°C: 20% (w/v) 60 Î·r at 20Â°C : 30% (w/v 180 Î·r at 20Â°C : 40% (w/v) 600 Î·r at 20Â°C : 50% (w/v)

Scale

Lab|Process

Lab

Lab|Process development|Process

Lab|Process development

Sample Loading Volume

Determined by desired degree of resolution [Adsorption Mode], ~2% total bed volume [Molecular Sizing], ~1% total bed volume [Partition Mode]

Theoretical Plates

> 48 000 m^-1

> 13000 m-1

> 43 000 m^-1

5000 m-1

> 38 000 m^-1

Signal Word

Warning

Danger

Typical Pressure Drop Over the Packed Bed

~ 3 MPa

~ 2 MPa

Noper Pack

100

Temperature

60°C

Residueafter Evaporation

5ppm max.

Shelf Life

24 months

Recommended maximum operational flow velocity

300 cm/h

Residue After Evaporation

5ppm max.

RIDADR

NONH for all modes of transport

Vapor Density

<1 (vs air)

Viscosity Max

Support System

Polypropylene (PP)

Volume Metric Usable

1.5 mL

Wetted Part

yes

Swelling factor

~1.7

Stokes Radius

~ 10

Suitability

Autosampler compatibility:Agilent Agilent 1050/1090A Agilent 1100/1200 Agilent 7673A, 7683 Agilent 5890/7985A/6890Hitachi AS-1000, AS-2000, AS-4000 L7200/L7250LEAP Technologies CTC LC PAL, GC PAL CombiPAL HTC PAL/HTS PALPerkinElmer Autosystem/AS-2000/XL

USP Type

Viscosity

1.98 mPa/s at 25Â°C

Volume Metric

4 mL

Volume Metric Total

2 mL

PurityGrade Notes

Mobile phase for HPLC applications

Synonym

DMSO

System

Sepax C-Pro|Sefia|Sepax 2

Volume Metric

2 mL

2.0 mL

Water

0.04% max.

Thread Size

10-425

20 mm

9 mm

Solubility

It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be

Vapor Pressure

17 mmHg ( 20 °C)

0.55mbar at 20Â°C

Volume English

1 dr.

Volume Metric Residual

<170µL

Total Time of Preparation

< 30 min/6 preps

U SPType

Total binding capacity

~50 mg hIgG/mL resin

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