Amersham ECL Western Blotting Detection Kit for detecting 1000 cm²
Detection of mouse and rabbit primary antibodies on western blots using HRPconjugated secondary antibodies and ECL reagents.
Optimized system provides rapid, sensitive results using simple protocols.
Designed for use with nitrocellulose and PVDF membranes.
High sensitivity: Up to 1NA times more sensitive than colorimetric methods.
Rapid: Results can be rapidly obtained on Xray film, providing hard copy data and avoiding fading associated with colorimetric detection methods.
Easy reprobing: Save time and materials by reprobing on membranes up to 1NA times.
Quantifiable: Linear response in the range NA.2 to 2 OD units using preflashed Hyperfilm ECL,
Nonradioactiveno specialized facilities neededProven performancethe most widely referenced chemiluminescent detection method with hundreds of publications worldwideECL Western Blotting System uses horseradish peroxidase (HRP) conjugated antimouse and antirabbit antibodies for luminolbased detection of Western blots. Blots probed with mouse or rabbit primary antibodies are incubated with HRPconjugated secondary antibodies. Addition of ECL Detection Reagents results in a chemiluminescent signal that can be captured on film or detected with a CCD camera.
ECL Western Blotting System is the world’s most widely used and referenced chemiluminescent immunodetection system.
Each system contains the following reagents, sufficient for detection of 1NANANA cm² membrane:
Antimouse HRP conjugate, 1NANA µl
Antirabbit HRP conjugate, 1NANA µl
ECL Detection Reagents 1 and 2, 62.5 ml each
Blocking agent, 5 g.